公开(公告)号：US10544425B2

公开(公告)日：2020-01-28

申请号：US13976526

申请日：2011-12-26

申请人： Hiroshi Kato ,  Hideo Maeda ,  Yoshihiro Sunohara ,  Ikuo Ando ,  Masahiro Oshima ,  Motoshige Kawata ,  Hitoshi Yoshida ,  Sakiko Hirose ,  Makiko Kawagishi ,  Yojiro Taniguchi ,  Kazumasa Murata ,  Hiroaki Maeda ,  Yuji Yamada ,  Keisuke Sekino ,  Akihiko Yamazaki

发明人： Hiroshi Kato ,  Hideo Maeda ,  Yoshihiro Sunohara ,  Ikuo Ando ,  Masahiro Oshima ,  Motoshige Kawata ,  Hitoshi Yoshida ,  Sakiko Hirose ,  Makiko Kawagishi ,  Yojiro Taniguchi ,  Kazumasa Murata ,  Hiroaki Maeda ,  Yuji Yamada ,  Keisuke Sekino ,  Akihiko Yamazaki

IPC分类号： C12N15/82 ,  C12Q1/6895

摘要： By a QTL analysis and so forth using 4-HPPD inhibitor-susceptible rice and 4-HPPD inhibitor-resistant rice, a hypothetical gene (HIS1 gene) of an iron/ascorbate-dependent oxidoreductase gene located on a short arm of chromosome 2 of rice has been identified as a 4-HPPD inhibitor-resistance gene. Further, it has also been revealed that a homologous gene (HSL1 gene) of the HIS1 gene is located on chromosome 6 of rice. Furthermore, it has been found out that utilizations of these genes make it possible to efficiently produce a plant having increased resistance or susceptibility to a 4-HPPD inhibitor and to efficiently determine whether a plant has resistance or susceptibility to a 4-HPPD inhibitor.

公开(公告)号：US20150047066A1

公开(公告)日：2015-02-12

申请号：US13976526

申请日：2011-12-26

申请人： Hiroshi Kato ,  Hideo Maeda ,  Yoshihiro Sunohara ,  Ikuo Ando ,  Masahiro Oshima ,  Motoshige Kawata ,  Hitoshi Yoshida ,  Sakiko Hirose ,  Makiko Kawagishi ,  Yojiro Taniguchi ,  Kazumasa Murata ,  Hiroaki Maeda ,  Yuji Yamada ,  Keisuke Sekino ,  Akihiko Yamazaki

发明人： Hiroshi Kato ,  Hideo Maeda ,  Yoshihiro Sunohara ,  Ikuo Ando ,  Masahiro Oshima ,  Motoshige Kawata ,  Hitoshi Yoshida ,  Sakiko Hirose ,  Makiko Kawagishi ,  Yojiro Taniguchi ,  Kazumasa Murata ,  Hiroaki Maeda ,  Yuji Yamada ,  Keisuke Sekino ,  Akihiko Yamazaki

IPC分类号： C12N15/82 ,  C12Q1/68

CPC分类号： C12N15/8274 ,  C12N9/0004 ,  C12Q1/6895

摘要： By a QTL analysis and so forth using 4-HPPD inhibitor-susceptible rice and 4-HPPD inhibitor-resistant rice, a hypothetical gene (HIS1 gene) of an iron/ascorbate-dependent oxidoreductase gene located on a short arm of chromosome 2 of rice has been identified as a 4-HPPD inhibitor-resistance gene. Further, it has also been revealed that a homologous gene (HSL1 gene) of the HIS1 gene is located on chromosome 6 of rice. Furthermore, it has been found out that utilizations of these genes make it possible to efficiently produce a plant having increased resistance or susceptibility to a 4-HPPD inhibitor and to efficiently determine whether a plant has resistance or susceptibility to a 4-HPPD inhibitor.

摘要翻译： 通过使用4-HPPD抑制剂敏感水稻和4-HPPD抑制剂抗性水稻的QTL分析等，位于水稻2号染色体短臂上的铁/抗坏血酸依赖性氧化还原酶基因的假设基因（HIS1基因） 已被鉴定为4-HPPD抑制剂抗性基因。 此外，还揭示了HIS1基因的同源基因（HSL1基因）位于水稻的6号染色体上。 此外，已经发现，这些基因的利用使得有可能有​​效地生产对4-HPPD抑制剂具有增加的抗性或易感性的植物，并有效地确定植物是否具有对4-HPPD抑制剂的抗性或易感性。

公开(公告)号：US08124406B2

公开(公告)日：2012-02-28

申请号：US12979823

申请日：2010-12-28

申请人： Kazuma Tomizuka ,  Hitoshi Yoshida ,  Kazunori Hanaoka ,  Mitsuo Oshimura ,  Isao Ishida ,  Yoshimi Kuroiwa

发明人： Kazuma Tomizuka ,  Hitoshi Yoshida ,  Kazunori Hanaoka ,  Mitsuo Oshimura ,  Isao Ishida ,  Yoshimi Kuroiwa

IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N15/01 ,  C12N15/64 ,  C07H21/04

CPC分类号： A01K67/0275 ,  A01K67/0271 ,  A01K67/0276 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/01 ,  A01K2267/03 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/873 ,  C12N15/90 ,  C12N15/907 ,  C12N2800/30

摘要： The present invention relates to a method for producing a modified foreign chromosome(s) or a fragment(s) thereof, which comprises the steps of: (a) preparing a microcell comprising a foreign chromosome(s) or a fragment(s) thereof, and transferring said foreign chromosome(s) or a fragment(s) into a cell with high homologous recombination efficiency through its fusion with said microcell; (b) in said cell with high homologous recombination efficiency, inserting a targeting vector by homologous recombination into a desired site of said foreign chromosome(s) or a fragment(s) thereof, and/or a desired site of a chromosome(s) derived from said cell with high homologous recombination efficiency, thereby marking said desired site; and (c) in said cell with high homologous recombination efficiency, causing deletion and/or translocation to occur at the marked site of said foreign chromosome(s) or a fragment(s) thereof.

摘要翻译： 本发明涉及一种生产修饰的外源染色体或其片段的方法，其包括以下步骤：（a）制备包含外来染色体或其片段的微细胞 并通过与所述微细胞融合将所述外源染色体或片段转移到具有高同源重组效率的细胞中; （b）在具有高同源重组效率的所述细胞中，通过同源重组将靶向载体插入所述外源染色体或其片段的所需位点和/或染色体的所需位点， 衍生自具有高同源重组效率的所述细胞，从而标记所述期望的位点; 和（c）在具有高同源重组效率的所述细胞中，导致在所述外源染色体或其片段的标记位点发生缺失和/或易位。

公开(公告)号：US08110671B2

公开(公告)日：2012-02-07

申请号：US11797904

申请日：2007-05-08

申请人： Kazuma Tomizuka ,  Hitoshi Yoshida ,  Kazunori Hanaoka ,  Mitsuo Oshimura ,  Isao Ishida

发明人： Kazuma Tomizuka ,  Hitoshi Yoshida ,  Kazunori Hanaoka ,  Mitsuo Oshimura ,  Isao Ishida

IPC分类号： C07H21/04

CPC分类号： A01K67/0275 ,  A01K67/0271 ,  A01K67/0276 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/01 ,  A01K2267/03 ,  C07K16/00 ,  C07K16/18 ,  C07K2317/21 ,  C12N15/8509 ,  C12N15/90 ,  C12N2517/02 ,  C12N2800/30

摘要： The specification relates to a method for producing a chimeric non-human animal, which comprises preparing a microcell containing a foreign chromosome(s) or a fragment(s) thereof and transferring the foreign chromosome(s) or fragment(s) thereof into a pluripotent cell by fusion with the microcell; a chimeric non-human animal which can be produced by the above method and its progeny; tissues and cells derived therefrom; and a method for using the same. Further, a pluripotent cell containing a foreign chromosome(s) or a fragment(s) thereof, a method for producing the same, and a method for using the same are also provided. Moreover, a pluripotent cell in which at least two endogenous genes are disrupted, and a method for producing the same by homologous recombination are provided.

摘要翻译： 本说明书涉及嵌合非人动物的制造方法，其包括制备含有外来染色体或其片段的微细胞，并将其外来的染色体或片段转移到 多细胞与微细胞融合; 可以通过上述方法及其后代产生的嵌合非人动物; 从其衍生的组织和细胞; 及其使用方法。 此外，还提供了含有外来染色体或其片段的多能细胞，其制备方法及其使用方法。 此外，提供其中至少两个内源基因被破坏的多能细胞，以及通过同源重组产生其的方法。

公开(公告)号：US07476536B2

公开(公告)日：2009-01-13

申请号：US10477471

申请日：2002-05-10

申请人： Yoshimi Kuroiwa ,  Kazuma Tomizuka ,  Hitoshi Yoshida ,  Isao Ishida

发明人： Yoshimi Kuroiwa ,  Kazuma Tomizuka ,  Hitoshi Yoshida ,  Isao Ishida

IPC分类号： C12N15/63 ,  C12N21/02 ,  C12N15/00

CPC分类号： A01K67/0278 ,  A01K67/0275 ,  A01K67/0276 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/052 ,  A01K2217/054 ,  A01K2217/15 ,  A01K2227/105 ,  A01K2267/01 ,  A01K2267/0381 ,  A61K48/00 ,  A61K2039/505 ,  C07K16/00 ,  C07K16/243 ,  C07K2317/21 ,  C12N15/8509 ,  C12N15/87 ,  C12N15/90 ,  C12N2800/30

摘要： The present invention relates to a human artificial chromosome which is genetically transmissible to the next generation with high efficiency and the method for using the same. More specifically, the present invention relates to: a human artificial chromosome in which an about 3.5 Mb to about 1 Mb region containing an antibody λ light chain gene derived from human chromosome 22 is bound to a chromosome fragment which is transmissible to a progeny through a germ line of a non-human animal, said chromosome fragment is derived from another human chromosome; a non-human animal carrying the human artificial chromosome and an offspring thereof; a method for producing the non-human animal; a method for producing a human antibody using the non-human animal or an offspring thereof; and a human antibody-producing mouse carrying the human artificial chromosome.

摘要翻译： 本发明涉及一种以高效率遗传传播到下一代的人类人造染色体及其使用方法。 更具体地，本发明涉及：人类人造染色体，其中含有源自人染色体22的抗体λ轻链基因的约3.5Mb至约1Mb区域与染色体片段结合，所述染色体片段可通过 生殖系的非人类动物，所述染色体片段衍生自另一个人染色体; 携带人类人造染色体的非人类动物及其后代; 一种生产非人动物的方法; 使用非人动物或其后代生产人抗体的方法; 和携带人类人造染色体的人抗体生产小鼠。

公开(公告)号：US20090007282A1

公开(公告)日：2009-01-01

申请号：US12116294

申请日：2008-05-07

申请人： Kazuma TOMIZUKA ,  Hitoshi Yoshida ,  Kazunori Hanaoka ,  Mitsuo Oshimura ,  Isao Ishida ,  Yoshimi Kuroiwa

发明人： Kazuma TOMIZUKA ,  Hitoshi Yoshida ,  Kazunori Hanaoka ,  Mitsuo Oshimura ,  Isao Ishida ,  Yoshimi Kuroiwa

IPC分类号： A01K67/027

CPC分类号： A01K67/0275 ,  A01K67/0271 ,  A01K67/0276 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/01 ,  A01K2267/03 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/873 ,  C12N15/90 ,  C12N15/907 ,  C12N2800/30

摘要： The present invention relates to a method for producing a modified foreign chromosome(s) or a fragment(s) thereof, which comprises the steps of: (a) preparing a microcell comprising a foreign chromosome(s) or a fragment(s) thereof, and transferring said foreign chromosome(s) or a fragment(s) into a cell with high homologous recombination efficiency through its fusion with said microcell; (b) in said cell with high homologous recombination efficiency, inserting a targeting vector by homologous recombination into a desired site of said foreign chromosome(s) or a fragment(s) thereof, and/or a desired site of a chromosome(s) derived from said cell with high homologous recombination efficiency, thereby marking said desired site; and (c) in said cell with high homologous recombination efficiency, causing deletion and/or translocation to occur at the marked site of said foreign chromosome(s) or a fragment(s) thereof.

摘要翻译： 本发明涉及一种生产修饰的外源染色体或其片段的方法，其包括以下步骤：（a）制备包含外来染色体或其片段的微细胞 并通过与所述微细胞融合将所述外源染色体或片段转移到具有高同源重组效率的细胞中; （b）在具有高同源重组效率的所述细胞中，通过同源重组将靶向载体插入所述外源染色体或其片段的所需位点和/或染色体的所需位点， 衍生自具有高同源重组效率的所述细胞，从而标记所述期望的位点; 和（c）在具有高同源重组效率的所述细胞中，导致在所述外源染色体或其片段的标记位点发生缺失和/或易位。

公开(公告)号：US20080317743A1

公开(公告)日：2008-12-25

申请号：US12140009

申请日：2008-06-16

申请人： Yoshimi Kuroiwa ,  Kazuma Tomizuka ,  Hitoshi Yoshida ,  Isao Ishida

发明人： Yoshimi Kuroiwa ,  Kazuma Tomizuka ,  Hitoshi Yoshida ,  Isao Ishida

IPC分类号： A61K39/395 ,  A01K67/027 ,  C12P21/08

CPC分类号： A01K67/0278 ,  A01K67/0275 ,  A01K67/0276 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/052 ,  A01K2217/054 ,  A01K2217/15 ,  A01K2227/105 ,  A01K2267/01 ,  A01K2267/0381 ,  A61K48/00 ,  A61K2039/505 ,  C07K16/00 ,  C07K16/243 ,  C07K2317/21 ,  C12N15/8509 ,  C12N15/87 ,  C12N15/90 ,  C12N2800/30

摘要： The present invention relates to a human artificial chromosome which is genetically transmissible to the next generation with high efficiency and the method for using the same. More specifically, the present invention relates to: a human artificial chromosome in which an about 3.5 Mb to about 1 Mb region containing an antibody λ light chain gene derived from human chromosome 22 is bound to a chromosome fragment which is transmissible to a progeny through a germ line of a non-human animal, said chromosome fragment is derived from another human chromosome; a non-human animal carrying the human artificial chromosome and an offspring thereof; a method for producing the non-human animal; a method for producing a human antibody using the nonhuman animal or an offspring thereof; and a human antibody-producing mouse carrying the human artificial chromosome.

摘要翻译： 本发明涉及一种以高效率遗传传播到下一代的人类人造染色体及其使用方法。 更具体地，本发明涉及：人类人造染色体，其中含有源自人染色体22的抗体λ轻链基因的约3.5Mb至约1Mb区域与染色体片段结合，所述染色体片段可通过 生殖系的非人类动物，所述染色体片段衍生自另一个人染色体; 携带人类人造染色体的非人类动物及其后代; 一种生产非人动物的方法; 使用非人动物或其后代生产人抗体的方法; 和携带人类人造染色体的人抗体生产小鼠。

公开(公告)号：USD577855S1

公开(公告)日：2008-09-30

申请号：US29260896

申请日：2006-06-05

申请人： Hitoshi Yoshida

设计人： Hitoshi Yoshida

公开(公告)号：US20060126208A1

公开(公告)日：2006-06-15

申请号：US11294996

申请日：2005-12-05

申请人： Nobuhito Kitamura ,  Hitoshi Yoshida

发明人： Nobuhito Kitamura ,  Hitoshi Yoshida

IPC分类号： G11B5/09 ,  G11B15/12 ,  G11B5/596

CPC分类号： G11B20/1217 ,  G11B5/3967 ,  G11B5/59655 ,  G11B2005/0002 ,  G11B2020/1277 ,  G11B2020/1287 ,  G11B2020/1292 ,  G11B2220/2516

摘要： Embodiments of the invention provide a magnetic disk drive and its control method capable of effectively using the unrecordable area which is caused due to the composite magnetic head's gap between the read head and the write head. In one embodiment, a disk drive comprises a composite magnetic head structured in such a manner that during write the write head WH reaches each servo area earlier than the read head RH, and a read write control unit to control the write and read done by the composite magnetic head. On the disk, common servo data SP to be used both during read and during write, write servo data SW to be used during write and read servo data SR to be used during read are recorded in this order in each servo area. During write, the read write control unit starts the write head recording data at the timing when reading write servo data SW from the servo area is completed.

公开(公告)号：US06632976B1

公开(公告)日：2003-10-14

申请号：US09033936

申请日：1998-03-02

申请人： Kazuma Tomizuka ,  Hitoshi Yoshida ,  Kazunori Hanaoka ,  Mitsuo Oshimura ,  Isao Ishida

发明人： Kazuma Tomizuka ,  Hitoshi Yoshida ,  Kazunori Hanaoka ,  Mitsuo Oshimura ,  Isao Ishida

IPC分类号： A01K67027

CPC分类号： A01K67/0275 ,  A01K67/0271 ,  A01K67/0276 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/01 ,  A01K2267/03 ,  C07K16/00 ,  C07K16/18 ,  C07K2317/21 ,  C12N15/8509 ,  C12N15/90 ,  C12N2517/02 ,  C12N2800/30

摘要： A chimeric, non-human animal can be produced by a method that entails providing a microcell that contains one or more foreign chromosomes or fragment(s) thereof and then fusing the microcell with a pluripotent cell, thereby introducing the foreign chromosome(s) or fragment(s) into the latter. The pluripotent cell thus obtained can be used to generate a chimeric, non-human animal, the cells, tissues, and/or progeny of which can be the source of a product, such as an antibody, that is associated with one or more genes on the foreign chromosome(s) or fragment(s).

摘要翻译： 嵌合的非人动物可以通过一种方法产生，该方法需要提供含有一个或多个外来染色体或其片段的微细胞，然后将微细胞与多能细胞融合，由此引入外源染色体或 片段进入后者。 由此获得的多能细胞可以用于产生嵌合的非人动物，其细胞，组织和/或后代可以是与一个或多个基因相关的产物例如抗体的来源 在外来染色体或片段上。