公开(公告)号：US12117440B2

公开(公告)日：2024-10-15

申请号：US16640753

申请日：2018-07-25

Applicant: Hiroshima University

Inventor： Akio Kuroda ,  Takenori Ishida

IPC: G01N33/543 ,  C07K7/06

CPC classification number: G01N33/54326 ,  C07K7/06

Abstract: The present invention provides a method for simply isolating exosomes from a sample containing exosomes. The present invention includes a complex forming step of forming a complex by binding an exosome in a sample to a particular peptide that contains lysines and is supported on a carrier and a dissociating step of dissociating the exosome from the complex by bringing the complex into contact with a dissociation buffer containing metal cations.

公开(公告)号：US09499822B2

公开(公告)日：2016-11-22

申请号：US14413035

申请日：2013-08-08

Applicant: Hiroshima University

Inventor： Akio Kuroda ,  Ryuichi Hirota ,  Takenori Ishida

IPC: C12N15/52 ,  C12N9/02 ,  C07K14/195 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20

CPC classification number: C12N15/52 ,  C07K14/195 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N9/0004 ,  C12Y120/01001

Abstract: A simple and inexpensive method for selectively culturing a microorganism which method makes it possible to selectively culture a microorganism of interest even without using a sterilization operation or an antibiotic substance is provided. The method according to the present invention selectively culturing a microorganism includes the step of culturing, in a culture medium containing phosphorous acid as a sole phosphorous source, a recombinant microorganism into which a phosphite dehydrogenase gene has been introduced.

Abstract translation: 提供了一种用于选择性培养微生物的简单和便宜的方法，该方法使得即使不使用灭菌操作或抗生素也可选择性培养感兴趣的微生物。 根据本发明的选择性培养微生物的方法包括在含有磷酸作为唯一磷源的培养基中培养引入了亚磷酸脱氢酶基因的重组微生物的步骤。