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公开(公告)号:US20090158469A1
公开(公告)日:2009-06-18
申请号:US11719509
申请日:2005-11-08
CPC分类号: C12N15/8277 , C12N9/1029 , C12N15/8274
摘要: To provide a novel herbicide-resistance gene and a plant imparted a resistance to herbicides by using this gene, a plant having a resistance to herbicides is constructed by transforming a plant cell by a DNA encoding the following protein (A) or (B) and then regenerating the thus transformed plant cell in a plant. (A) A protein having the amino acid sequence represented by SEQ ID NO:2. (B) A protein having an amino acid sequence of SEQ ID NO:2 including substitution, deletion, insertion or addition of one or plural amino acids and having a herbicide-resistant activity.
摘要翻译: 为了提供新型除草剂抗性基因和通过使用该基因赋予除草剂抗性的植物,通过用编码以下蛋白质(A)或(B)的DNA转化植物细胞和(B)和 然后在植物中再生这样转化的植物细胞。 (A)具有SEQ ID NO:2所示的氨基酸序列的蛋白质。 (B)具有SEQ ID NO:2的氨基酸序列的蛋白质,包括取代,缺失,插入或添加一个或多个氨基酸并具有除草剂抗性活性。
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公开(公告)号:US07795505B2
公开(公告)日:2010-09-14
申请号:US11719509
申请日:2005-11-08
CPC分类号: C12N15/8277 , C12N9/1029 , C12N15/8274
摘要: A DNA encoding a phosphinothricin acetyltransferase protein obtained from Streptomyces sp. AB3534 strain is described which imparts a resistance to herbicides, particularly the herbicide phosphinothricin (PPT). Plants having a herbicide resistance are constructed by transforming a plant cell with DNA encoding the phosphinothricin acetyltransferase protein and then regenerating the transformed plant cell into a plant. A recombinant protein having the herbicide-resistant activity is also described.
摘要翻译: 编码从链霉菌属(Streptomyces sp。)获得的膦丝菌素乙酰转移酶蛋白质的DNA 描述了对除草剂,特别是除草剂膦丝菌素(PPT)具有抗性的AB3534菌株。 通过用编码膦丝菌素乙酰转移酶蛋白的DNA转化植物细胞,然后将转化的植物细胞再生成植物来构建具有除草剂抗性的植物。 还描述了具有除草剂抗性活性的重组蛋白。
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公开(公告)号:US5714643A
公开(公告)日:1998-02-03
申请号:US596131
申请日:1996-02-09
申请人: Kiyoshi Sato , Makoto Yoshida , Kenji Kanbe , Kiyoshi Hirazawa , Tomio Takeuchi
发明人: Kiyoshi Sato , Makoto Yoshida , Kenji Kanbe , Kiyoshi Hirazawa , Tomio Takeuchi
CPC分类号: C07C35/16 , C07C29/10 , C07B2200/07 , C07C2101/14
摘要: An aqueous solution of kasugamycin or its salt is either mixed with a strongly acidic ion-exchange resin of the H.sup.+ -form in a reaction vessel, or is brought into contact with a strongly acidic ion-exchange resin of the H.sup.+ -form in a column containing said resin, followed by heating said aqueous solution and the resin under an atmospheric pressure or an elevated pressure, thereby to effect hydrolytic reaction of kasugamycin. By this reaction, an acidic reaction solution containing D-chiro-inositol is produced. This acidic reaction solution containing D-chiro-inositol is separated from the strongly acidic ion-exchange resin. Subsequently, D-chiro-inositol is recovered from the reaction solution. For this recovery, the acidic reaction solution is passed successively through a column of a strongly acidic ion-exchange resin of the H.sup.+ -form and through a column of a strongly basic ion exchange resin of the OH.sup.- -form, so that a neutral aqueous eluate containing D-chiro-inositol is obtained. This neutral aqueous eluate is then concentrated and crystals of D-chiro-inositol are made to deposit, whereby D-chiro-inositol of a high purity can be harvested with high efficiency.
摘要翻译: PCT No.PCT / JP94 / 01304第 371日期1996年2月9日 102(e)日期1996年2月9日PCT 1994年8月8日PCT PCT。 公开号WO95 / 04711 日期1995年2月16日将春日霉素或其盐的水溶液与H +型的强酸性离子交换树脂在反应容器中混合,或与H +型强酸离子交换树脂接触, - 在含有所述树脂的柱中,然后在大气压或高压下加热所述水溶液和树脂,从而进行春日霉素的水解反应。 通过该反应,生成含有D-手性肌醇的酸性反应液。 从强酸性离子交换树脂中分离含有D-手性肌醇的酸性反应溶液。 随后,从反应溶液中回收D-手性肌醇。 对于这种回收,酸性反应溶液依次通过H +型强酸性离子交换树脂柱,并通过OH-形式的强碱性离子交换树脂柱,使得中性水性 得到含有D-手性肌醇的洗脱液。 然后将该中性水性洗脱液浓缩,并使D-手性肌醇晶体沉积,从而高效率地收获高纯度的D-手性肌醇。
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