公开(公告)号：US09139860B2

公开(公告)日：2015-09-22

申请号：US13084148

申请日：2011-04-11

申请人： Hong Gu Chun

发明人： Hong Gu Chun

IPC分类号： C07H21/00 ,  C07H21/04 ,  C12Q1/00 ,  C12Q1/68 ,  C12Q1/70

CPC分类号： C12Q1/00 ,  C12Q1/682 ,  C12Q1/703 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2565/1015

摘要： The present invention relates to a self-amplifying folded oligonucleotide structure for sensitive oligonucleotide sensing without polymerase chain reaction (PCR). A self-amplifying folded oligonucleotide structure comprise a target sensing sequence having stem loop structure, a signaling molecule and signal modifying molecule labeled two stems wherein the two stems include oligonucleotide sequence that is complementary to a target sensing sequence of another self-amplifying folded oligonucleotide structure.

摘要翻译： 本发明涉及用于无聚合酶链反应（PCR）的敏感寡核苷酸检测的自扩增折叠寡核苷酸结构。 自扩增折叠的寡核苷酸结构包含具有茎环结构的目标感测序列，标记为两个茎的信号分子和信号修饰分子，其中两个茎包括与另一个自扩增折叠寡核苷酸结构的靶感测序列互补的寡核苷酸序列 。

公开(公告)号：US20120259104A1

公开(公告)日：2012-10-11

申请号：US13084148

申请日：2011-04-11

申请人： Hong Gu Chun

发明人： Hong Gu Chun

IPC分类号： C07H21/00

CPC分类号： C12Q1/00 ,  C12Q1/682 ,  C12Q1/703 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2565/1015

摘要： The present invention relates to a self-amplifying folded oligonucleotide structure for sensitive oligonucleotide sensing without polymerase chain reaction (PCR). A self-amplifying folded oligonucleotide structure comprise a target sensing sequence having stem loop structure, a signaling molecule and signal modifying molecule labeled two stems wherein the two stems include oligonucleotide sequence that is complementary to a target sensing sequence of another self-amplifying folded oligonucleotide structure.

摘要翻译： 本发明涉及用于无聚合酶链反应（PCR）的敏感寡核苷酸检测的自扩增折叠寡核苷酸结构。 自扩增折叠的寡核苷酸结构包含具有茎环结构的目标感测序列，标记为两个茎的信号分子和信号修饰分子，其中两个茎包括与另一个自扩增折叠寡核苷酸结构的靶感测序列互补的寡核苷酸序列 。

公开(公告)号：US20090104689A1

公开(公告)日：2009-04-23

申请号：US11791648

申请日：2005-11-25

申请人： Hee-Chan Kim ,  Taek Dong Chung ,  Hong Gu Chun

发明人： Hee-Chan Kim ,  Taek Dong Chung ,  Hong Gu Chun

IPC分类号： C12M1/00 ,  G01N27/00

CPC分类号： G01N15/1031 ,  B01L3/5027 ,  G01N15/1056

摘要： The present invention relates to a microchip using polyelectrolyte salt bridge for cytometry, velocimetry, and cell sorting. The microchip comprises; a) an inlet for solution to be analyzed, b) a microchannel which provides a moving passage for solution to be analyzed, c) at least one outlet for solution to be analyzed which has passed through the moving passage, d) at least one electrode system comprising a first and a second salt bridges connected to the microchannel (the two salt bridges face each other), and a first and a second reservoirs connected to said each salt bridge (the reservoir comprises electrode and standard electrolyte solution). The microchip detects analytes in the solution to be analyzed (for example, a cell) by detecting change of impedance. In detail, anion in the standard electrolyte solution, which is comprised in the first reservoir, moves from the first salt bridge to the second salt bridge across the microchannel. Impedance change occurs by interference of anion moving across the microchannel and the change can be detected by impedance analyzer connected to electrodes in the first and the second reservoirs.

摘要翻译： 本发明涉及使用聚电解质盐桥用于细胞计数，速度测定和细胞分选的微芯片。 微芯片包括： a）待分析溶液的入口，b）提供待分析溶液的移动通道的微通道，c）至少一个已经通过移动通道的待分析溶液出口，d）至少一个电极 系统，包括连接到微通道（两个盐桥彼此面对）的第一和第二盐桥，以及连接到所述每个盐桥（所述储存器包括电极和标准电解质溶液）的第一和第二储存器。 微芯片通过检测阻抗的变化来检测要分析的解决方案中的分析物（例如，单元）。 详细地，包含在第一储存器中的标准电解质溶液中的阴离子通过微通道从第一盐桥移动到第二盐桥。 阻抗变化通过阴离子移动穿过微通道的干扰而发生，并且可以通过与第一和第二储存器中的电极连接的阻抗分析仪来检测变化。