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    Electrochemical assay for cis-diols
    1.
    发明授权
    Electrochemical assay for cis-diols 失效
    顺式二醇的电化学测定

    公开(公告)号：US4820636A

    公开(公告)日：1989-04-11

    申请号：US832452

    申请日：1986-02-21

    申请人： Hugh A. O. Hill ,  Monika J. Green

    发明人： Hugh A. O. Hill ,  Monika J. Green

    IPC分类号： C12Q1/00 ,  G01N33/487 ,  G01N33/66 ,  G01N33/72 ,  C12Q1/54 ,  C07F9/02 ,  C12Q1/48

    CPC分类号： G01N33/723 ,  C12Q1/004 ,  G01N33/66 ,  Y10S435/817

    摘要： The invention disclosed relates to an electrochemical assay which is particularly concerned with an assay for the presence of, or amount of, glycosylated haemoglobin in a blood sample, but which extends to a general assay for detecting the presence of, monitoring the level of or determining the concentration of compounds containing cis-diol groups, such as glycols, nucleic acid components, sugars, polyols, catechols and glycosylated proteins.When ferrocene boronic acid or a derivative thereof is present free in solution, it can act as a mediator for a wide range of oxido-reductases. However in the presence of, for example, sugars containing the cis-diol structure, the metallocene (such as ferrocene boronic acid) covalently binds to the sugar. When the ferrocene boronic acid is thus bound, it is essentially immobilized and its properties as a mediator and ability to diffuse will be markedly affected.

    摘要翻译： 所公开的本发明涉及一种电化学测定法，其特别涉及血液样品中糖基化血红蛋白的存在或量的测定，其扩展到用于检测存在或监测血液样品的水平或确定 含有顺式二醇基团的化合物如二醇，核酸组分，糖，多元醇，邻苯二酚和糖基化蛋白质的浓度。 当二茂铁硼酸或其衍生物在溶液中游离时，可以作为广泛范围氧化还原酶的介质。 然而，在例如含有顺式二醇结构的糖的存在下，金属茂（如二茂铁硼酸）与糖共价结合。 当二茂铁硼酸结合时，其基本上是固定的，其作为介体的性质和扩散能力将受到显着影响。

    Assay for salicylate
    3.
    发明授权
    Assay for salicylate 失效
    测定水杨酸盐

    公开(公告)号：US4777132A

    公开(公告)日：1988-10-11

    申请号：US847955

    申请日：1986-04-03

    申请人： Monika J. Green ,  Philip N. B. Gibbs

    发明人： Monika J. Green ,  Philip N. B. Gibbs

    IPC分类号： G01N27/327 ,  C12N9/02 ,  C12Q1/26 ,  G01N27/27 ,  G01N27/30 ,  G01N27/416 ,  G01N27/48

    CPC分类号： C12N9/0073 ,  C12Q1/26 ,  C12Y114/13001 ,  Y10S435/817

    摘要： The specification discloses a method and apparatus for the assay of salicylate (or a derivative thereof) comprising a conducting body having at a surface thereof an enzyme capable of catalyzing the conversion of salicylate (or a derivative thereof) to a catechol whereby said catechol is subject to direct electrochemical measurement as it oxidizes at said surface to generate a current as a measure of the reaction taking place and thereby of the concentration of salicylate at said surface. The method typically comprises the steps of;(a) treating a liquid sample suspected of containing salicylate or a derivative thereof with an enzyme capable of catalyzing the conversion of salicylate or a derivative thereof into a catechol, and,(b) measuring the concentration of catechol in the treated sample by direct electrochemistry.

    摘要翻译： 本说明书公开了一种用于测定水杨酸（或其衍生物）的方法和装置，其包含导电体，其表面具有能够催化水杨酸酯（或其衍生物）转化为邻苯二酚的酶，由此所述儿茶酚是受试者 直接电化学测量，因为它在所述表面处氧化以产生电流，作为所发生的反应的测量，从而在所述表面处产生水杨酸盐的浓度。 该方法通常包括以下步骤： （a）用能够催化水杨酸酯或其衍生物转化成儿茶酚的酶处理怀疑含有水杨酸酯或其衍生物的液体样品，和（b）通过直接电化学测量处理的样品中的儿茶酚浓度 。

    Electrochemical assay for haemoglobin
    4.
    发明授权
    Electrochemical assay for haemoglobin 失效
    血红蛋白电化学测定

    公开(公告)号：US4876205A

    公开(公告)日：1989-10-24

    申请号：US84983

    申请日：1987-08-12

    申请人： Monika J. Green ,  Roger J. Lias

    发明人： Monika J. Green ,  Roger J. Lias

    IPC分类号： G01N27/416 ,  G01N27/49 ,  G01N33/487 ,  G01N33/72

    CPC分类号： G01N33/721

    摘要： A method for the assay of haemoglobin in blood is provided in which the blood is contacted with a sufficient amount of a ferricyanide and the haemoglobin is electrochemically assayed by monitoring the current changes produced on reduction of the ferricyanide by the haemoglobin.

    摘要翻译： 提供血液中血红蛋白测定方法，其中血液与足量的铁氰化物接触，血红蛋白通过监测由血红蛋白还原铁氰化物产生的当前变化进行电化学测定。