公开(公告)号：US20140274784A1

公开(公告)日：2014-09-18

申请号：US14215720

申请日：2014-03-17

申请人： Hycor Biomedical, Inc.

发明人： Mark David Van Cleve ,  Taylor Addison Reid ,  Linda Marie Trondle ,  Victoria Hung ,  Yi Luo ,  Dennis Edwin Rieger ,  Evan Phillip McMenamy ,  Nanditha Raghavan ,  Morkoah Blay Reliford ,  Douglas John Canfield ,  Yiling Nie ,  Edsel Lawrence Noche Sinson ,  Scott William Vande Wetering ,  Teri Taylor ,  Travis Knox ,  Fran Zylo Cuaresma Jacalne ,  James Weston ,  Jennifer Bao-Guey Chan ,  Stephanie TuViVo ,  Rachel Sarah Schell ,  Ronald Norman Diamond ,  Steve Michael Gann ,  Eric Darnell Hall ,  Tae Ho Hwang ,  John Lewis Morton ,  Anatoly Moskalev ,  Marinela Gombosev Stack ,  Bruce Alan Sargeant

IPC分类号： G01N33/564 ,  G01N33/543 ,  G01N33/68

CPC分类号： G01N21/645 ,  G01N21/6428 ,  G01N21/76 ,  G01N33/5306 ,  G01N33/54326 ,  G01N33/5434 ,  G01N33/54393 ,  G01N33/564 ,  G01N33/569 ,  G01N33/5695 ,  G01N33/56983 ,  G01N33/582 ,  G01N33/6854 ,  G01N33/6893 ,  G01N35/0098 ,  G01N35/1011 ,  G01N2021/6484 ,  G01N2035/0453 ,  G01N2035/1062 ,  G01N2201/062 ,  G01N2201/08 ,  G01N2333/4703 ,  G01N2333/62 ,  G01N2333/78 ,  G01N2800/24 ,  Y10T436/119163

摘要： A quantitative method for performing an automated diagnostic assay, comprising: incubating a capture reagent with a streptavidin-coated medium to form a solid phase complex; washing the solid phase complex to remove excess capture reagent; incubating the solid phase complex with a serum sample to form an immune complex; washing the immune complex to remove any unbound sample; incubating the immune complex with a conjugate to create an immune-conjugate complex; washing the immune-conjugate complex to remove any unbound conjugate; introducing a substrate capable of generating a quantifiable response; and calibrating the response generated from introducing the substrate.

摘要翻译： 一种用于进行自动诊断测定的定量方法，包括：将捕获试剂与链霉抗生物素蛋白包被的培养基孵育以形成固相络合物; 洗涤固相络合物以除去多余的捕获试剂; 将固相络合物与血清样品孵育以形成免疫复合物; 洗涤免疫复合物以去除任何未结合的样品; 将免疫复合物与缀合物一起孵育以产生免疫共轭复合物; 洗涤免疫共轭复合物以除去任何未结合的结合物; 引入能够产生可量化反应的基底; 并校准由引入衬底产生的响应。