公开(公告)号：US20140273277A1

公开(公告)日：2014-09-18

申请号：US14215861

申请日：2014-03-17

申请人： Hycor Biomedical, Inc.

发明人： Ronald Norman Diamond ,  Steve Michael Gann ,  Eric Darnell Hall ,  Tae Ho Hwang ,  John Lewis Morton ,  Anatoly Moskalev ,  Bruce Alan Sargeant ,  Dennis Edwin Rieger ,  Marinela Gombosev ,  Mark David Van Cleve

IPC分类号： G01N35/10 ,  G01N21/64

CPC分类号： G01N21/645 ,  G01N21/6428 ,  G01N21/76 ,  G01N33/5306 ,  G01N33/54326 ,  G01N33/5434 ,  G01N33/54393 ,  G01N33/564 ,  G01N33/569 ,  G01N33/5695 ,  G01N33/56983 ,  G01N33/582 ,  G01N33/6854 ,  G01N33/6893 ,  G01N35/0098 ,  G01N35/1011 ,  G01N2021/6484 ,  G01N2035/0453 ,  G01N2035/1062 ,  G01N2201/062 ,  G01N2201/08 ,  G01N2333/4703 ,  G01N2333/62 ,  G01N2333/78 ,  G01N2800/24 ,  Y10T436/119163

摘要： An apparatus for measuring the luminescence and the fluorescence of a sample, comprising: a light tight optics box capable of receiving a pipette tip containing a sample; an optical sensor located within the optics box and capable of being disposed in both a luminescence reading position and a fluorescence reading position; an excitation light fiber optic bundle and a sample transmission fiber optic bundle; an excitation light assembly that projects excitation light onto a first terminus end of the excitation light fiber optic bundle; and an in-line filter located along the sample transmission fiber optic bundle.

摘要翻译： 一种用于测量样品的发光和荧光的装置，包括：能够接收含有样品的移液管尖端的不透光光学盒; 光学传感器位于光学盒内并且能够被布置在发光读取位置和荧光读取位置; 激发光纤光束和样品传输光纤束; 激发光组件，其将激发光投射到激发光纤光束的第一末端; 以及沿着样品传输光纤束定位的串联滤波器。

公开(公告)号：US09766233B2

公开(公告)日：2017-09-19

申请号：US14634061

申请日：2015-02-27

申请人： Hycor Biomedical, Inc.

发明人： Ronald Norman Diamond ,  Steve Michael Gann ,  Eric Darnell Hall ,  Tae Ho Hwang ,  John Lewis Morton ,  Anatoly Moskalev ,  Bruce Alan Sargeant ,  Dennis Edwin Rieger ,  Marinela Gombosev ,  Mark David Van Cleve

IPC分类号： G01N21/64 ,  G01N33/564 ,  G01N35/10 ,  G01N33/543 ,  G01N33/68 ,  G01N33/53 ,  G01N33/58 ,  G01N35/00 ,  G01N21/76 ,  G01N33/569 ,  G01N35/04

CPC分类号： G01N21/645 ,  G01N21/6428 ,  G01N21/76 ,  G01N33/5306 ,  G01N33/54326 ,  G01N33/5434 ,  G01N33/54393 ,  G01N33/564 ,  G01N33/569 ,  G01N33/5695 ,  G01N33/56983 ,  G01N33/582 ,  G01N33/6854 ,  G01N33/6893 ,  G01N35/0098 ,  G01N35/1011 ,  G01N2021/6484 ,  G01N2035/0453 ,  G01N2035/1062 ,  G01N2201/062 ,  G01N2201/08 ,  G01N2333/4703 ,  G01N2333/62 ,  G01N2333/78 ,  G01N2800/24 ,  Y10T436/119163

摘要： Apparatuses and methods of optically analyzing fluid within a pipette are described herein. In an embodiment, an optical reader subassembly includes a housing including an internal area, a container configured to hold a fluid sample at a sample position in a light tight manner within the internal area of the housing, a light source configured to project light onto the fluid sample within the container, and an optical sensor configured to move between different sensor positions while the fluid sample remains stationary at the sample position, the different sensor positions including at least two of: (i) a first sensor position for taking a luminescence reading of the fluid sample; (ii) a second sensor position for taking a dark current or other background measurement; and (iii) a third sensor position for taking a fluorescence reading of the fluid sample.

公开(公告)号：US09753033B2

公开(公告)日：2017-09-05

申请号：US14634011

申请日：2015-02-27

申请人： Hycor Biomedical, Inc.

发明人： Ronald Norman Diamond ,  Steve Michael Gann ,  Eric Darnell Hall ,  Tae Ho Hwang ,  John Lewis Morton ,  Anatoly Moskalev ,  Bruce Alan Sargeant ,  Dennis Edwin Rieger ,  Marinela Gombosev ,  Mark David Van Cleve

IPC分类号： G01N21/64 ,  G01N33/564 ,  G01N35/10 ,  G01N33/543 ,  G01N33/68 ,  G01N33/53 ,  G01N33/58 ,  G01N35/00 ,  G01N21/76 ,  G01N33/569 ,  G01N35/04

CPC分类号： G01N21/645 ,  G01N21/6428 ,  G01N21/76 ,  G01N33/5306 ,  G01N33/54326 ,  G01N33/5434 ,  G01N33/54393 ,  G01N33/564 ,  G01N33/569 ,  G01N33/5695 ,  G01N33/56983 ,  G01N33/582 ,  G01N33/6854 ,  G01N33/6893 ,  G01N35/0098 ,  G01N35/1011 ,  G01N2021/6484 ,  G01N2035/0453 ,  G01N2035/1062 ,  G01N2201/062 ,  G01N2201/08 ,  G01N2333/4703 ,  G01N2333/62 ,  G01N2333/78 ,  G01N2800/24 ,  Y10T436/119163

摘要： Apparatuses and methods of optically analyzing fluid within a pipette are described herein. In an embodiment, an optical reader subassembly includes a pipette having a tip, the pipette configured to aspirate a fluid sample and hold the fluid sample within the tip, a housing including a reentrant seal and an internal area, the housing configured to receive at least the tip of the pipette through the reentrant seal so that the tip of the pipette is located in a light tight manner within the internal area, a light source positioned to be in proximity to the tip of the pipette when the tip of the pipette is received by the housing, the light source configured to project light through the tip of the pipette and onto the fluid sample held within the tip, and an optical sensor configured to take a reading of the fluid sample held within the tip of the pipette without any of the fluid sample being injected from the pipette.

公开(公告)号：US09075055B2

公开(公告)日：2015-07-07

申请号：US14215861

申请日：2014-03-17

申请人： Hycor Biomedical, Inc.

发明人： Ronald Norman Diamond ,  Steve Michael Gann ,  Eric Darnell Hall ,  Tae Ho Hwang ,  John Lewis Morton ,  Anatoly Moskalev ,  Bruce Alan Sargeant ,  Dennis Edwin Rieger ,  Marinela Gombosev ,  Mark David Van Cleve

IPC分类号： G01N35/00 ,  G01N33/564 ,  G01N35/10 ,  G01N21/64 ,  G01N33/543 ,  G01N33/68 ,  G01N33/53 ,  G01N33/58 ,  G01N35/04

CPC分类号： G01N21/645 ,  G01N21/6428 ,  G01N21/76 ,  G01N33/5306 ,  G01N33/54326 ,  G01N33/5434 ,  G01N33/54393 ,  G01N33/564 ,  G01N33/569 ,  G01N33/5695 ,  G01N33/56983 ,  G01N33/582 ,  G01N33/6854 ,  G01N33/6893 ,  G01N35/0098 ,  G01N35/1011 ,  G01N2021/6484 ,  G01N2035/0453 ,  G01N2035/1062 ,  G01N2201/062 ,  G01N2201/08 ,  G01N2333/4703 ,  G01N2333/62 ,  G01N2333/78 ,  G01N2800/24 ,  Y10T436/119163

摘要： An apparatus for measuring the luminescence and the fluorescence of a sample, comprising: a light tight optics box capable of receiving a pipette tip containing a sample; an optical sensor located within the optics box and capable of being disposed in both a luminescence reading position and a fluorescence reading position; an excitation light fiber optic bundle and a sample transmission fiber optic bundle; an excitation light assembly that projects excitation light onto a first terminus end of the excitation light fiber optic bundle; and an in-line filter located along the sample transmission fiber optic bundle.

摘要翻译： 一种用于测量样品的发光和荧光的装置，包括：能够接收含有样品的移液管尖端的不透光光学盒; 光学传感器位于光学盒内并且能够被配置在发光读取位置和荧光读取位置; 激发光纤光束和样品传输光纤束; 激发光组件，其将激发光投射到激发光纤光束的第一末端; 以及沿着样品传输光纤束定位的串联滤波器。

公开(公告)号：US20150177146A1

公开(公告)日：2015-06-25

申请号：US14634061

申请日：2015-02-27

申请人： Hycor Biomedical, Inc.

发明人： Ronald Norman Diamond ,  Steve Michael Gann ,  Eric Darnell Hall ,  Tae Ho Hwang ,  John Lewis Morton ,  Anatoly Moskalev ,  Bruce Alan Sargeant ,  Dennis Edwin Rieger ,  Marinela Gombosev ,  Mark David Van Cleve

IPC分类号： G01N21/64

CPC分类号： G01N21/645 ,  G01N21/6428 ,  G01N21/76 ,  G01N33/5306 ,  G01N33/54326 ,  G01N33/5434 ,  G01N33/54393 ,  G01N33/564 ,  G01N33/569 ,  G01N33/5695 ,  G01N33/56983 ,  G01N33/582 ,  G01N33/6854 ,  G01N33/6893 ,  G01N35/0098 ,  G01N35/1011 ,  G01N2021/6484 ,  G01N2035/0453 ,  G01N2035/1062 ,  G01N2201/062 ,  G01N2201/08 ,  G01N2333/4703 ,  G01N2333/62 ,  G01N2333/78 ,  G01N2800/24 ,  Y10T436/119163

摘要： Apparatuses and methods of optically analyzing fluid within a pipette are described herein. In an embodiment, an optical reader subassembly includes a housing including an internal area, a container configured to hold a fluid sample at a sample position in a light tight manner within the internal area of the housing, a light source configured to project light onto the fluid sample within the container, and an optical sensor configured to move between different sensor positions while the fluid sample remains stationary at the sample position, the different sensor positions including at least two of: (i) a first sensor position for taking a luminescence reading of the fluid sample; (ii) a second sensor position for taking a dark current or other background measurement; and (iii) a third sensor position for taking a fluorescence reading of the fluid sample.

摘要翻译： 本文描述了在移液管内光学分析流体的装置和方法。 在一个实施例中，光学读取器子组件包括壳体，其包括内部区域，被配置为将样品位置处的样品位置以不透光的方式保持在壳体的内部区域内的容器，配置成将光投射到 容器内的流体样品和配置成在不同传感器位置之间移动的光学传感器，同时流体样品保持静止在样品位置，不同的传感器位置包括以下中的至少两个：（i）用于获取发光读数的第一传感器位置 的流体样品; （ii）用于进行暗电流或其他背景测量的第二传感器位置; 和（iii）用于获取流体样品的荧光读数的第三传感器位置。

公开(公告)号：US20150177145A1

公开(公告)日：2015-06-25

申请号：US14634011

申请日：2015-02-27

申请人： Hycor Biomedical, Inc.

发明人： Ronald Norman Diamond ,  Steve Michael Gann ,  Eric Darnell Hall ,  Tae Ho Hwang ,  John Lewis Morton ,  Anatoly Moskalev ,  Bruce Alan Sargeant ,  Dennis Edwin Rieger ,  Marinela Gombosev ,  Mark David Van Cleve

IPC分类号： G01N21/64

CPC分类号： G01N21/645 ,  G01N21/6428 ,  G01N21/76 ,  G01N33/5306 ,  G01N33/54326 ,  G01N33/5434 ,  G01N33/54393 ,  G01N33/564 ,  G01N33/569 ,  G01N33/5695 ,  G01N33/56983 ,  G01N33/582 ,  G01N33/6854 ,  G01N33/6893 ,  G01N35/0098 ,  G01N35/1011 ,  G01N2021/6484 ,  G01N2035/0453 ,  G01N2035/1062 ,  G01N2201/062 ,  G01N2201/08 ,  G01N2333/4703 ,  G01N2333/62 ,  G01N2333/78 ,  G01N2800/24 ,  Y10T436/119163

摘要： Apparatuses and methods of optically analyzing fluid within a pipette are described herein. In an embodiment, an optical reader subassembly includes a pipette having a tip, the pipette configured to aspirate a fluid sample and hold the fluid sample within the tip, a housing including a reentrant seal and an internal area, the housing configured to receive at least the tip of the pipette through the reentrant seal so that the tip of the pipette is located in a light tight manner within the internal area, a light source positioned to be in proximity to the tip of the pipette when the tip of the pipette is received by the housing, the light source configured to project light through the tip of the pipette and onto the fluid sample held within the tip, and an optical sensor configured to take a reading of the fluid sample held within the tip of the pipette without any of the fluid sample being injected from the pipette.

摘要翻译： 本文描述了在移液管内光学分析流体的装置和方法。 在一个实施例中，光学读取器子组件包括具有尖端的移液管，移液管被配置为吸入流体样品并将流体样本保持在尖端内，壳体包括可折入密封件和内部区域，壳体被配置为至少接收 移液管的尖端通过折入密封，使得移液管的尖端在内部区域内以不透光的方式定位，当接收到移液管的尖端时，定位成接近移液管的尖端的光源 通过所述壳体，所述光源被配置为将光投射通过所述移液管的尖端并且保持在所述尖端内的所述流体样本上;以及光学传感器，被配置为读取保持在所述移液管的尖端内的所述流体样品，而没有任何 流体样品从移液管注射。

公开(公告)号：US20140274784A1

公开(公告)日：2014-09-18

申请号：US14215720

申请日：2014-03-17

申请人： Hycor Biomedical, Inc.

发明人： Mark David Van Cleve ,  Taylor Addison Reid ,  Linda Marie Trondle ,  Victoria Hung ,  Yi Luo ,  Dennis Edwin Rieger ,  Evan Phillip McMenamy ,  Nanditha Raghavan ,  Morkoah Blay Reliford ,  Douglas John Canfield ,  Yiling Nie ,  Edsel Lawrence Noche Sinson ,  Scott William Vande Wetering ,  Teri Taylor ,  Travis Knox ,  Fran Zylo Cuaresma Jacalne ,  James Weston ,  Jennifer Bao-Guey Chan ,  Stephanie TuViVo ,  Rachel Sarah Schell ,  Ronald Norman Diamond ,  Steve Michael Gann ,  Eric Darnell Hall ,  Tae Ho Hwang ,  John Lewis Morton ,  Anatoly Moskalev ,  Marinela Gombosev Stack ,  Bruce Alan Sargeant

IPC分类号： G01N33/564 ,  G01N33/543 ,  G01N33/68

CPC分类号： G01N21/645 ,  G01N21/6428 ,  G01N21/76 ,  G01N33/5306 ,  G01N33/54326 ,  G01N33/5434 ,  G01N33/54393 ,  G01N33/564 ,  G01N33/569 ,  G01N33/5695 ,  G01N33/56983 ,  G01N33/582 ,  G01N33/6854 ,  G01N33/6893 ,  G01N35/0098 ,  G01N35/1011 ,  G01N2021/6484 ,  G01N2035/0453 ,  G01N2035/1062 ,  G01N2201/062 ,  G01N2201/08 ,  G01N2333/4703 ,  G01N2333/62 ,  G01N2333/78 ,  G01N2800/24 ,  Y10T436/119163

摘要： A quantitative method for performing an automated diagnostic assay, comprising: incubating a capture reagent with a streptavidin-coated medium to form a solid phase complex; washing the solid phase complex to remove excess capture reagent; incubating the solid phase complex with a serum sample to form an immune complex; washing the immune complex to remove any unbound sample; incubating the immune complex with a conjugate to create an immune-conjugate complex; washing the immune-conjugate complex to remove any unbound conjugate; introducing a substrate capable of generating a quantifiable response; and calibrating the response generated from introducing the substrate.

摘要翻译： 一种用于进行自动诊断测定的定量方法，包括：将捕获试剂与链霉抗生物素蛋白包被的培养基孵育以形成固相络合物; 洗涤固相络合物以除去多余的捕获试剂; 将固相络合物与血清样品孵育以形成免疫复合物; 洗涤免疫复合物以去除任何未结合的样品; 将免疫复合物与缀合物一起孵育以产生免疫共轭复合物; 洗涤免疫共轭复合物以除去任何未结合的结合物; 引入能够产生可量化反应的基底; 并校准由引入衬底产生的响应。