公开(公告)号：US20110053292A1

公开(公告)日：2011-03-03

申请号：US12937139

申请日：2009-04-10

申请人： Hyung-Soon Park ,  Sang-Wan So ,  Woon-Seok Yeo ,  Soo-Jae Lee ,  Jung-Rok Lee ,  Ju-Hee Lee ,  Kwang-Pyo Kim

发明人： Hyung-Soon Park ,  Sang-Wan So ,  Woon-Seok Yeo ,  Soo-Jae Lee ,  Jung-Rok Lee ,  Ju-Hee Lee ,  Kwang-Pyo Kim

IPC分类号： G01N33/543 ,  C12N9/10 ,  C07K14/435 ,  C07K16/00

CPC分类号： G01N33/553 ,  G01N33/585 ,  G01N33/6848 ,  G01N2458/15 ,  H01J49/00 ,  Y10T436/24

摘要： There is provided a novel method for amplifying mass spectrometric signals. More particularly, a novel method for detecting signals of a target molecule includes: i) allowing a test sample, in which it is required to determine whether or not a target molecule is present, to be contact with a gold particle whose surface is modified to selectively bind to the target molecule, ii) allowing a low molecular molecule engrafted to the gold particle to generate mass spectrometric signals after the interaction, such as binding, between the gold particle and the target molecule, and iii) amplifying the mass spectrometric signals by generating a great deal of mass spectrometric signals of the low molecular molecule even in the presence of a trace of the target molecule. Also, the assay system using the method and the gold particle prepared in the method are provided. The method may be useful to specifically amplify signals of the target molecule without any pretreatment of a test sample, which makes it possible to measure the target molecule simply and precisely.

摘要翻译： 提供了一种扩增质谱信号的新方法。 更具体地，用于检测靶分子的信号的新方法包括：i）允许测试样品，其中需要确定靶分子是否存在，以将其表面修饰的金颗粒与 选择性地结合靶分子，ii）允许移植到金颗粒上的低分子分子在金颗粒和靶分子之间的相互作用之后产生质谱信号，例如结合，以及iii）通过以下步骤扩增质谱信号： 甚至在痕量的靶分子的存在下也产生大量低分子分子的质谱信号。 此外，提供了使用该方法的测定系统和该方法中制备的金颗粒。 该方法可用于特异性扩增靶分子的信号而无需对测试样品进行任何预处理，这使得可以简单且精确地测量目标分子。

公开(公告)号：US08673653B2

公开(公告)日：2014-03-18

申请号：US12937139

申请日：2009-04-10

申请人： Hyung-Soon Park ,  Sang-Wan So ,  Woon-Seok Yeo ,  Soo-Jae Lee ,  Jung-Rok Lee ,  Ju-Hee o Lee ,  Kwang-Pyo Kim

发明人： Hyung-Soon Park ,  Sang-Wan So ,  Woon-Seok Yeo ,  Soo-Jae Lee ,  Jung-Rok Lee ,  Ju-Hee o Lee ,  Kwang-Pyo Kim

IPC分类号： G01N33/553

CPC分类号： G01N33/553 ,  G01N33/585 ,  G01N33/6848 ,  G01N2458/15 ,  H01J49/00 ,  Y10T436/24

摘要： Provided is a novel method for amplifying mass spectrometric signals. A novel method for detecting signals of a target molecule includes: allowing a sample, which comprises a target molecule, to contact a gold particle having a surface modified to selectively bind the target molecule, allowing a low molecular weight molecule engrafted to the gold particle generate mass spectrometric signals after the interaction, e.g., binding, between the gold particle and the target molecule, and amplifying the mass spectrometric signals to generate much mass spectrometric signals of the low molecular weight molecule even when trace amounts of the target molecule are present. An assay system using the method and the gold particle prepared in the method are provided. The method amplifies signals of the target molecule without pretreatment of a sample, making it possible to measure the target molecule simply and precisely.

摘要翻译： 提供了一种扩增质谱信号的新方法。 用于检测靶分子信号的新方法包括：允许包含目标分子的样品接触具有修饰以选择性结合靶分子的表面的金颗粒，允许移植到金颗粒的低分子量分子产生 即使存在痕量的目标分子，即使在金颗粒与靶分子之间的相互作用之后的质谱信号，也可以扩增质谱信号以产生低分子量分子的大量质谱信号。 提供了使用该方法的测定系统和该方法中制备的金颗粒。 该方法在不预处理样品的情况下放大靶分子的信号，使得可以简单且准确地测量目标分子。