公开(公告)号：US20230408439A1

公开(公告)日：2023-12-21

申请号：US18208834

申请日：2023-06-12

Applicant: ILLUMINA, INC.

Inventor： Xu Liu ,  Lisa Savagian ,  Arvin Emadi

IPC: G01N27/327 ,  G01N33/487 ,  G01N27/419

CPC classification number: G01N27/3278 ,  G01N27/419 ,  G01N33/48721

Abstract: An example of a nanopore sensing system includes an application specific integrated circuit (ASIC) sensor mounted on a printed circuit board having an electrical interface with the ASIC sensor; and a nanopore sequencer formed on the ASIC sensor. The nanopore sequencer includes a redox mediator chamber having a cis electrode positioned therein; a cis well; a membrane positioned between the cis well and the redox mediator chamber, the membrane to confine a redox mediator species in the redox mediator chamber and to allow an ionic species to pass between the redox mediator chamber and the cis well; a plurality of trans wells, each including a trans electrode positioned therein; and a plurality of nanopores respectively fluidically connecting the cis well to each of the plurality of trans wells.

公开(公告)号：US20250109434A1

公开(公告)日：2025-04-03

申请号：US18900392

申请日：2024-09-27

Applicant: Illumina, Inc.

Inventor： Hugo Brandão ,  John Moon ,  Jeffrey G. Mandell ,  Erin Garcia ,  Lisa Savagian ,  Eunho Noh ,  Xiangyuan Yang ,  Sharyuen Soh ,  Sadeer Salam

IPC: C12Q1/6869 ,  C12Q1/6809

Abstract: A polymer is disposed through a nanopore that encodes a polynucleotide's sequence and includes monomer units that encode nucleotides and include first and second reporter moieties and first and second arresting constructs. The first reporter moiety is translocated into the nanopore's aperture, where a first value of an electrical property of the first reporter moiety is measured while the first arresting construct pauses translocation. The second reporter moiety is translocated into the aperture, where a second value of an electrical property of the second reporter moiety is measured while the second arresting construct pauses translocation. The first value and the second value for each monomer unit is used to identify the nucleotide encoded by that monomer unit; and distinguish the nucleotide encoded by that monomer unit from the nucleotides encoded by adjacent monomer units, including by those that encode the same type of nucleotide as that monomer unit.

公开(公告)号：US20240280557A1

公开(公告)日：2024-08-22

申请号：US18552532

申请日：2022-03-10

Applicant: Illumina, Inc.

Inventor： Lisa Savagian ,  Burton Simpson ,  Sang Park ,  Boyan Boyanov ,  Jeffrey G. Mandell ,  Seth M. McDonald

IPC: G01N33/487 ,  B82B1/00 ,  B82B3/00 ,  C12Q1/6869

CPC classification number: G01N33/48721 ,  B82B1/008 ,  B82B3/0038 ,  C12Q1/6869

Abstract: Polypeptide nanopores synthetically functionalized with positively charged species, and methods of making and using the same, are provided herein. In some examples, a polypeptide nanopore includes a first side, a second side, a channel extending through the first and second sides, and a mutated amino acid residue. The mutated amino acid residue may be synthetically functionalized with a positively charged species that inhibits translocation of cations through the channel.

公开(公告)号：US20240207890A1

公开(公告)日：2024-06-27

申请号：US18543320

申请日：2023-12-18

Applicant: Illumina, Inc.

Inventor： Yannan Zhao ,  Florika Macazo ,  Rachel Goodridge ,  Emily Welch ,  Hassan Bohra ,  Sang Park ,  Lisa Savagian ,  Borhan Chueh ,  Thomas Brubaker ,  Shreshtha Mishra

IPC: B05D1/38 ,  B05D7/24

CPC classification number: B05D1/38 ,  B05D7/24 ,  B05D7/22

Abstract: A method for modifying an interstitial surface separating recesses from one another can include flowing a first fluid over the interstitial surface and into the recesses, such that the interstitial surface is substantially coated with the first fluid and the recesses are substantially filled with the first fluid; and while the first fluid remains within the recesses, replacing the first fluid coating the interstitial surface with a second fluid comprising a reagent.