公开(公告)号：US11975325B2

公开(公告)日：2024-05-07

申请号：US17358783

申请日：2021-06-25

Applicant: ILLUMINA, INC.

Inventor： Sean M. Ramirez ,  Anmiv Prabhu ,  Rigo Pantoja ,  Michelle Higgins

IPC: C12Q1/6813 ,  B01L3/00 ,  B01L7/00 ,  G01N35/10

CPC classification number: B01L3/502738 ,  B01L7/00 ,  G01N35/1097 ,  B01L2300/0877 ,  B01L2300/1805 ,  C12Q1/6813

Abstract: An example of a kit includes a flow cell assembly. The flow cell assembly includes a reaction chamber, a temperature controlled flow channel in selective fluid communication with an inlet of the reaction chamber, and a filter positioned in the temperature controlled flow channel. The reaction chamber includes depressions separated by interstitial regions and capture primers attached within each of the depressions. The filter is i) to block concentrated biological sample-polymer complexes generated in the temperature controlled flow channel at a first temperature, and ii) to allow passage of concentrated biological sample and polymer released from the complexes in the temperature controlled flow channel at a second temperature.

公开(公告)号：US20240307874A1

公开(公告)日：2024-09-19

申请号：US18651564

申请日：2024-04-30

Applicant: ILLUMINA, INC.

Inventor： Sean M. Ramirez ,  Anmiv Prabhu ,  Rigo Pantoja ,  Michelle Higgins

IPC: B01L3/00 ,  B01L7/00 ,  C12Q1/6813 ,  G01N35/10

CPC classification number: B01L3/502738 ,  B01L7/00 ,  G01N35/1097 ,  B01L2300/0877 ,  B01L2300/1805 ,  C12Q1/6813

Abstract: An example of a kit includes a flow cell assembly. The flow cell assembly includes a reaction chamber, a temperature controlled flow channel in selective fluid communication with an inlet of the reaction chamber, and a filter positioned in the temperature controlled flow channel. The reaction chamber includes depressions separated by interstitial regions and capture primers attached within each of the depressions. The filter is i) to block concentrated biological sample-polymer complexes generated in the temperature controlled flow channel at a first temperature, and ii) to allow passage of concentrated biological sample and polymer released from the complexes in the temperature controlled flow channel at a second temperature.

公开(公告)号：US20220001384A1

公开(公告)日：2022-01-06

申请号：US17358783

申请日：2021-06-25

Applicant: ILLUMINA, INC.

Inventor： Sean M. Ramirez ,  Anmiv Prabhu ,  Rigo Pantoja ,  Michelle Higgins

IPC: B01L3/00 ,  B01L7/00 ,  G01N35/10

Abstract: An example of a kit includes a flow cell assembly. The flow cell assembly includes a reaction chamber, a temperature controlled flow channel in selective fluid communication with an inlet of the reaction chamber, and a filter positioned in the temperature controlled flow channel. The reaction chamber includes depressions separated by interstitial regions and capture primers attached within each of the depressions. The filter is i) to block concentrated biological sample-polymer complexes generated in the temperature controlled flow channel at a first temperature, and ii) to allow passage of concentrated biological sample and polymer released from the complexes in the temperature controlled flow channel at a second temperature.