公开(公告)号：US20230279469A1

公开(公告)日：2023-09-07

申请号：US18146320

申请日：2022-12-23

申请人： ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA, INC. ,  Illumina Singapore Pte. Ltd.

发明人： Johan Sebastian Basuki ,  Jonathan Mark Boutell ,  Jeffrey S. Fisher ,  Louise Jane Fraser ,  Wayne N. George ,  Niall Anthony Gormley ,  David Jones ,  Xiaoyu Ma ,  Maria Ines Martins Vitoriano ,  Zhong Mei ,  Oliver Jon Miller ,  Andrew Price ,  Sebastien Georg Gabriel Ricoult ,  Vicki S. Thomson ,  Jacqueline C. Weir ,  Xiaoy Ma ,  Weihua Chang ,  Hui Han

IPC分类号： C12Q1/6806 ,  C12N15/10 ,  B01L3/00

CPC分类号： C12Q1/6806 ,  B01L3/502761 ,  C12N15/1065 ,  B01L2200/0647 ,  B01L2300/0893

摘要： An example of a flow cell includes a substrate having depressions separated by interstitial regions. First and second primers are immobilized within the depressions. First transposome complexes are immobilized within the depressions, and the first transposome complexes include a first amplification domain. Second transposome complexes are also immobilized within the depressions, and the second transposome complexes include a second amplification domain. Some of the first transposome complexes, or some of the second transposome complexes, or some of both of the first and second transposome complexes include a modification to reduce tagmentation efficiency.