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公开(公告)号：US20230279469A1
公开(公告)日：2023-09-07
申请号：US18146320
申请日：2022-12-23
发明人： Johan Sebastian Basuki , Jonathan Mark Boutell , Jeffrey S. Fisher , Louise Jane Fraser , Wayne N. George , Niall Anthony Gormley , David Jones , Xiaoyu Ma , Maria Ines Martins Vitoriano , Zhong Mei , Oliver Jon Miller , Andrew Price , Sebastien Georg Gabriel Ricoult , Vicki S. Thomson , Jacqueline C. Weir , Xiaoy Ma , Weihua Chang , Hui Han
IPC分类号： C12Q1/6806 , C12N15/10 , B01L3/00
CPC分类号： C12Q1/6806 , B01L3/502761 , C12N15/1065 , B01L2200/0647 , B01L2300/0893
摘要： An example of a flow cell includes a substrate having depressions separated by interstitial regions. First and second primers are immobilized within the depressions. First transposome complexes are immobilized within the depressions, and the first transposome complexes include a first amplification domain. Second transposome complexes are also immobilized within the depressions, and the second transposome complexes include a second amplification domain. Some of the first transposome complexes, or some of the second transposome complexes, or some of both of the first and second transposome complexes include a modification to reduce tagmentation efficiency.
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公开(公告)号：US20230302422A1
公开(公告)日：2023-09-28
申请号：US18187608
申请日：2023-03-21
发明人： Zhong Mei , Adithya Ramanitharapandian , Zhonghuan Luo , Hui Han
IPC分类号： B01J19/00 , C12Q1/6874
CPC分类号： B01J19/0046 , C12Q1/6874 , B01J2219/00596 , G03F7/0002
摘要： A sequencing kit includes a plurality of particles and a flow cell. The plurality of particles includes a primer set attached to a surface of each of the plurality of particles; and a flow cell surface attachment mechanism attached to the surface of each of the plurality of particles. The flow cell surface attachment mechanism is selected from the group consisting of a capture primer, an alkene, an alkyne, biotin, and a charged polymer. The flow cell includes a plurality of chemical pads that are spatially separated from one another on a substantially flat substrate surface, each of the chemical pads including chemistry to attach to the surface attachment mechanism.
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公开(公告)号：US11427868B2
公开(公告)日：2022-08-30
申请号：US16482613
申请日：2018-01-31
申请人： Illumina, Inc.
发明人： John S. Vieceli , Alex Nemiroski , Paul Belitz , Robert Langlois , M. Shane Bowen , Danny Yuan Chan , Bala Murali K. Venkatesan , Hui Han , Kevan Samiee , Stephen Tanner
IPC分类号： C12Q1/6874 , G01N21/64
摘要： Fiducial markers are provided on patterned arrays of the type that may be used for molecular analysis, such as sequencing. The fiducials may have configurations that enhance their detection in image or detection data, that facilitate or improve processing, that provide encoding of useful information, and so forth. Examples of the fiducials may include a non-closed shape that may encode information, allow for bubbles to escape during manufacture, and provide additional advantages over closed shape fiducials.
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4.发明申请公开(公告)号：US20220098653A1
公开(公告)日：2022-03-31
申请号：US17548246
申请日：2021-12-10
申请人： ILLUMINA, INC.
IPC分类号： C12Q1/6844 , C12Q1/6834 , C12N15/09 , B01J19/00 , B01L3/00 , C07K1/04 , C12Q1/6813 , C12Q1/6825 , C12Q1/6837 , C40B50/18
摘要： Structured substrate including (a) a plurality of nanoparticles distributed on a solid support, (b) a gel material forming a layer in association with the plurality of nanoparticles, and (c) a library of target nucleic acids in the gel material.
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公开(公告)号：US11262307B2
公开(公告)日：2022-03-01
申请号：US16482653
申请日：2018-01-31
申请人： Illumina, Inc.
发明人： John S. Vieceli , Alex Nemiroski , Paul Belitz , Robert Langlois , M. Shane Bowen , Danny Yuan Chan , Bala Murali K. Venkatesan , Hui Han , Kevan Samiee , Stephen Tanner
摘要： Fiducial markers are provided on patterned arrays of the type that may be used for molecular analysis, such as sequencing. The fiducial markers may have configurations that enhance their detection in image or detection data, that facilitate or improve processing, that provide encoding of useful information, and so forth. Examples of the fiducial markers may include features and materials that are provided on or in the support of a patterned array and that return at least a portion of incident light by reflection. The fiducial markers may form gratings or other encoding configurations that assist in image processing, alignment, or other aspects of processing of the patterned array.
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公开(公告)号：US20180085752A1
公开(公告)日：2018-03-29
申请号：US15700731
申请日：2017-09-11
申请人： Illumina, Inc.
发明人： Hui Han , Dajun A. Yuan , M. Shane Bowen
CPC分类号： B01L3/502707 , B01L2200/12 , B01L2300/0816 , B01L2300/0877 , B01L2300/0887 , B01L2300/0896 , B29C65/1406 , B29C65/1612 , B32B17/064 , B81C1/0038 , B81C2201/0153 , G03F7/0002 , G03F7/038 , G03F7/16 , G03F7/2002 , G03F7/30 , H01J37/32009 , H01J2237/3341
摘要： Imprinted substrates are often used to produce miniaturized devices for use in electrical, optic and biochemical applications. Imprinting techniques, such as nanoimprinting lithography, may leave residues in the surface of substrates that affect bonding and decrease the quality of the produced devices. An imprinted substrate with residue-free region, or regions with a reduced amount of residue for improved bonding quality is introduced. Methods to produce imprinted substrates without residues from the imprinting process are also introduced. Methods include physical exclusion methods, selective etching methods and energy application methods. These methods may produce residue-free regions in the surface of the substrate that can be used to produce higher strength bonding.
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公开(公告)号：US11835460B2
公开(公告)日：2023-12-05
申请号：US16482638
申请日：2018-01-31
申请人： Illumina, Inc.
发明人： John S. Vieceli , Alex Nemiroski , Paul Belitz , Robert Langlois , M. Shane Bowen , Danny Yuan Chan , Bala Murali K. Venkatesan , Hui Han , Kevan Samiee , Stephen Tanner
IPC分类号： G01N21/64 , B01J19/00 , C12Q1/6837
CPC分类号： G01N21/6456 , B01J19/0046 , C12Q1/6837 , G01N21/6452 , B01J2219/0056 , B01J2219/00317 , B01J2219/00529 , B01J2219/00547 , B01J2219/00549 , B01J2219/00576 , B01J2219/00608 , B01J2219/00648 , B01J2219/00722 , B01L2300/021 , G01N2021/6419 , G01N2021/6421 , H01J2237/24578
摘要： Fiducial markers are provided on patterned arrays of the type that may be used for molecular analysis, such as sequencing. The fiducials may have configurations and layouts that enhance their detection in image or detection data, that facilitate or improve processing, that provide encoding of useful information, and so forth. Examples of the fiducials may include offset layouts that may be useful in detecting the fiducials in different types and approaches in imaging, and that may help to distinguish regions of the array from one another in image data.
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公开(公告)号：US20220414853A1
公开(公告)日：2022-12-29
申请号：US17845519
申请日：2022-06-21
发明人： John S. Vieceli , Hui Han , Robert Langlois , Bo Lu
摘要： Registration of a patterned flow cell may utilize fiducials comprising sets or groupings of features (e.g., sites, sample wells, nanowells) having known locations and in which the placement of the features is not in accordance with a periodic pattern or is otherwise distinguishable from the periodic pattern of sites present in non-fiducial regions of the flow cell substrate. In certain embodiments the positioning of the sites that are part of the fiducial represent a break or discontinuity in the periodic pattern of sites that are otherwise present on the surface of a patterned flow cell.
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公开(公告)号：US20200248252A1
公开(公告)日：2020-08-06
申请号：US16482613
申请日：2018-01-31
申请人： Illumina, Inc.
发明人： John S. Vieceli , Alex Nemiroski , Paul Belitz , Robert Langlois , M. Shane Bowen , Danny Yuan Chan , Bala Murali K. Venkatesan , Hui Han , Kevan Samiee , Stephen Tanner
IPC分类号： C12Q1/6874 , G01N21/64
摘要： Fiducial markers are provided on patterned arrays of the type that may be used for molecular analysis, such as sequencing. The fiducials may have configurations that enhance their detection in image or detection data, that facilitate or improve processing, that provide encoding of useful information, and so forth. Examples of the fiducials may include a non-closed shape that may encode information, allow for bubbles to escape during manufacture, and provide additional advantages over closed shape fiducials.
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公开(公告)号：US20200064266A1
公开(公告)日：2020-02-27
申请号：US16482653
申请日：2018-01-31
申请人： Illumina, Inc.
发明人： John S. Vieceli , Alex Nemiroski , Paul Belitz , Robert Langlois , M. Shane Bowen , Danny Yuan Chan , Bala Murali K. Venkatesan , Hui Han , Kevan Samiee , Stephen Tanner
IPC分类号： G01N21/64
摘要： Fiducial markers are provided on patterned arrays of the type that may be used for molecular analysis, such as sequencing. The fiducials may have configurations that enhance their detection in image or detection data, that facilitate or improve processing, that provide encoding of useful information, and so forth. Examples of the fiducials may include features and materials that are provided on or in the support of the array and that return at least a portion of incident light by reflection. The fiducials may form gratings or other encoding configurations that assist in image processing, alignment, or other aspects of processing of the array.
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