公开(公告)号：US20230279469A1

公开(公告)日：2023-09-07

申请号：US18146320

申请日：2022-12-23

申请人： ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA, INC. ,  Illumina Singapore Pte. Ltd.

发明人： Johan Sebastian Basuki ,  Jonathan Mark Boutell ,  Jeffrey S. Fisher ,  Louise Jane Fraser ,  Wayne N. George ,  Niall Anthony Gormley ,  David Jones ,  Xiaoyu Ma ,  Maria Ines Martins Vitoriano ,  Zhong Mei ,  Oliver Jon Miller ,  Andrew Price ,  Sebastien Georg Gabriel Ricoult ,  Vicki S. Thomson ,  Jacqueline C. Weir ,  Xiaoy Ma ,  Weihua Chang ,  Hui Han

IPC分类号： C12Q1/6806 ,  C12N15/10 ,  B01L3/00

CPC分类号： C12Q1/6806 ,  B01L3/502761 ,  C12N15/1065 ,  B01L2200/0647 ,  B01L2300/0893

摘要： An example of a flow cell includes a substrate having depressions separated by interstitial regions. First and second primers are immobilized within the depressions. First transposome complexes are immobilized within the depressions, and the first transposome complexes include a first amplification domain. Second transposome complexes are also immobilized within the depressions, and the second transposome complexes include a second amplification domain. Some of the first transposome complexes, or some of the second transposome complexes, or some of both of the first and second transposome complexes include a modification to reduce tagmentation efficiency.

公开(公告)号：US20240191292A1

公开(公告)日：2024-06-13

申请号：US18523636

申请日：2023-11-29

申请人： ILLUMINA, INC.

发明人： Johan Sebastian Basuki ,  Hassan Bohra ,  Hui Kheng Karen Goh ,  Yin Nah Teo ,  Wei Wei

IPC分类号： C12Q1/6874 ,  B01L3/00

CPC分类号： C12Q1/6874 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/16 ,  B01L2300/0861

摘要： An example flow cell includes a substrate including depressions separated by interstitial regions; a polymeric hydrogel positioned within each of the depressions; a primer set attached to the polymeric hydrogel; and one of: a passivation component attached to the interstitial regions, or a passivation component attached to the polymeric hydrogel, or respective passivation components attached to each of the interstitial regions and the polymeric hydrogel.

公开(公告)号：US20240207846A1

公开(公告)日：2024-06-27

申请号：US18542447

申请日：2023-12-15

申请人： ILLUMINA, INC.

发明人： Johan Sebastian Basuki ,  Tyler J. Dill ,  Justin Fullerton ,  Tanmay Ghonge ,  Brian D. Mather ,  Wei Wei

IPC分类号： B01L3/00

CPC分类号： B01L3/502715 ,  B01L3/502753 ,  B01L2300/0645 ,  B01L2300/0887

摘要： An example of a flow cell includes a substrate; a plurality of reactive regions spatially separated from one another across the substrate; and a plurality of independently removable coatings respectively positioned over each of the plurality of reactive regions. Each of the plurality of reactive regions includes a polymeric hydrogel layer; and a reactive entity attached to the polymeric hydrogel layer.

公开(公告)号：US20240207842A1

公开(公告)日：2024-06-27

申请号：US18542511

申请日：2023-12-15

申请人： ILLUMINA, INC.

发明人： Gianluca Andrea Artioli ,  Johan Sebastian Basuki ,  Boyan Boyanov ,  Gabriele Canzi ,  Sahngki Hong ,  Nam Nguyen ,  Sebastien Georg Gabriel Ricoult ,  Alexandra Szemjonov ,  Xavier von Hatten

IPC分类号： B01L3/00

CPC分类号： B01L3/5027 ,  B01L2200/025 ,  B01L2300/12 ,  B01L2300/16 ,  B01L2300/1805

摘要： An example of a flow cell includes a substrate; a plurality of reactive regions spatially separated from one another across the substrate; and a plurality of independently removable coatings respectively positioned over each of the plurality of reactive regions. Each of the plurality of reactive regions includes a polymeric hydrogel layer; and a reactive entity attached to the polymeric hydrogel layer. At least one of the independently removable coatings is a gas-dissolvable coating.

公开(公告)号：US20240207839A1

公开(公告)日：2024-06-27

申请号：US18542470

申请日：2023-12-15

申请人： ILLUMINA, INC.

发明人： Johan Sebastian Basuki ,  Brian D. Mather ,  Sebastien Georg Gabriel Ricoult ,  Alexandra Szemjonov

IPC分类号： B01L3/00

CPC分类号： B01L3/502 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0877 ,  B01L2300/165

摘要： An example of a flow cell includes a substrate; a plurality of reactive regions spatially separated from one another across the substrate; and a plurality of independently removable coatings respectively positioned over each of the plurality of reactive regions. Each of the plurality of reactive regions includes a polymeric hydrogel layer; and a reactive entity attached to the polymeric hydrogel layer. At least one of the independently removable coatings is a composite that includes a thermo-responsive polymer and a photo-thermal additive.