公开(公告)号：US20140065702A1

公开(公告)日：2014-03-06

申请号：US13902060

申请日：2013-05-24

Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE

Inventor： Jane SC TSAI ,  Nikolay SERGEEV ,  Chih-Hsiang SUNG ,  Tseng-Huang LIU ,  Ting-Hsuan CHEN

IPC: B01L7/00

CPC classification number: B01L7/52 ,  B01L3/5082 ,  B01L7/525 ,  B01L7/54 ,  B01L2300/1883 ,  B01L2400/0445

Abstract: A polymerase chain reaction (PCR) device for a reverse transcriptase reaction (RT) and a convectively-driven polymerase chain reaction (cPCR) in the same device is provided. The PCR device comprises an upper temperature-controlling unit, a middle temperature-controlling unit and a lower temperature-controlling unit. The middle temperature-controlling unit is used for controlling a temperature of a reaction mixture contained in a reaction container to have a temperature for the reverse transcriptase reaction. The middle temperature-controlling unit is disposed between the upper temperature-controlling unit and the lower temperature-controlling unit. The upper temperature-controlling unit and the lower temperature-controlling unit are used for simultaneously controlling the reaction mixture contained in the reaction container to have a temperature gradient and a convection condition for the convectively-driven polymerase chain reaction.

Abstract translation: 提供了用于逆转录酶反应（RT）和对流驱动的聚合酶链反应（cPCR）在同一装置中的聚合酶链式反应（PCR）装置。 PCR装置包括上部温度控制单元，中间温度控制单元和下部温度控制单元。 中温控制单元用于控制反应容器中包含的反应混合物的温度以具有逆转录酶反应的温度。 中间温度控制单元设置在上部温度控制单元和下部温度控制单元之间。 上部温度控制单元和下部温度控制单元用于同时控制反应容器中包含的反应混合物具有用于对流驱动的聚合酶链式反应的温度梯度和对流条件。

公开(公告)号：US20240219269A1

公开(公告)日：2024-07-04

申请号：US18091952

申请日：2022-12-30

Applicant: Industrial Technology Research Institute

Inventor： Tseng-Huang LIU ,  Nien-Jen CHOU ,  Yuh-Tyng TSAI ,  Yi-Yun YUAN ,  Chia-Ying TANG

IPC: G01N1/14

CPC classification number: G01N1/14

Abstract: A biological sample pretreatment device includes a base body and a moveable part. The base body is formed with an elongated accommodation space configured to accommodate a sampling tube and a reaction tube aligned with each other. The moveable part moveably connects to the base body for ejecting the reaction tube from the base body.

公开(公告)号：US20240327895A1

公开(公告)日：2024-10-03

申请号：US18400228

申请日：2023-12-29

Applicant: Industrial Technology Research Institute

Inventor： Tseng-Huang LIU ,  Chien-Ming HSU ,  Chih-Hung LEE

IPC: C12Q1/6806

CPC classification number: C12Q1/6806

Abstract: A composition for stabilizing cell-free nucleic acid and/or exosomal nucleic acid in blood is provided. The composition for stabilizing cell-free nucleic acid and/or exosomal nucleic acid in blood is (a) a first composition or (b) a second composition. The first composition includes (i) an antiseptic component including an allantoin-formaldehyde condensation product; (ii) an enzyme inhibitory component including a first metal chelator and (iii) a metabolic inhibitory component including NaF or sodium azide. The second composition includes (i) an antiseptic component including an allantoin-formaldehyde condensate; (ii) an enzyme inhibiting component including a first metal chelator; (iii) a metabolic an inhibitory component including NaF or sodium azide; and (iv) an anticoagulant component including a second metal chelator.

公开(公告)号：US20210230600A1

公开(公告)日：2021-07-29

申请号：US17132793

申请日：2020-12-23

Applicant: Industrial Technology Research Institute

Inventor： Ping-Fu CHENG ,  Ya-Ling CHIU ,  Kang-Li WANG ,  Po-Yen LIN ,  Shih-Ta CHEN ,  Tseng-Huang LIU ,  Pei-Shin JIANG

IPC: C12N15/115 ,  C12N15/113 ,  A61K47/54 ,  A61K9/00 ,  A61P35/00

Abstract: A nucleic acid-drug complex is provided in the present disclosure, which includes a nucleic acid sequence of an anti-PD-L1 aptamer and a CpG oligonucleotide sequence capable of activating TLR9, in which the CpG oligonucleotide sequence consists of a first fragment and a second fragment, and the nucleic acid sequence of the anti-PD-L1 aptamer is inserted between the first fragment and the second fragment.