公开(公告)号：US20140065702A1

公开(公告)日：2014-03-06

申请号：US13902060

申请日：2013-05-24

Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE

Inventor： Jane SC TSAI ,  Nikolay SERGEEV ,  Chih-Hsiang SUNG ,  Tseng-Huang LIU ,  Ting-Hsuan CHEN

IPC: B01L7/00

CPC classification number: B01L7/52 ,  B01L3/5082 ,  B01L7/525 ,  B01L7/54 ,  B01L2300/1883 ,  B01L2400/0445

Abstract: A polymerase chain reaction (PCR) device for a reverse transcriptase reaction (RT) and a convectively-driven polymerase chain reaction (cPCR) in the same device is provided. The PCR device comprises an upper temperature-controlling unit, a middle temperature-controlling unit and a lower temperature-controlling unit. The middle temperature-controlling unit is used for controlling a temperature of a reaction mixture contained in a reaction container to have a temperature for the reverse transcriptase reaction. The middle temperature-controlling unit is disposed between the upper temperature-controlling unit and the lower temperature-controlling unit. The upper temperature-controlling unit and the lower temperature-controlling unit are used for simultaneously controlling the reaction mixture contained in the reaction container to have a temperature gradient and a convection condition for the convectively-driven polymerase chain reaction.

Abstract translation: 提供了用于逆转录酶反应（RT）和对流驱动的聚合酶链反应（cPCR）在同一装置中的聚合酶链式反应（PCR）装置。 PCR装置包括上部温度控制单元，中间温度控制单元和下部温度控制单元。 中温控制单元用于控制反应容器中包含的反应混合物的温度以具有逆转录酶反应的温度。 中间温度控制单元设置在上部温度控制单元和下部温度控制单元之间。 上部温度控制单元和下部温度控制单元用于同时控制反应容器中包含的反应混合物具有用于对流驱动的聚合酶链式反应的温度梯度和对流条件。

公开(公告)号：US20180104696A1

公开(公告)日：2018-04-19

申请号：US15588043

申请日：2017-05-05

Applicant: INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE

Inventor： Tseng-Huang Liu ,  Kuo-Hsing Wen ,  Pei-Shin Jiang ,  Jie-Len Huang ,  Ting-Hsuan CHEN

IPC: B01L7/00 ,  C12Q1/68

CPC classification number: B01L7/52 ,  B01L7/525 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0832 ,  B01L2300/18 ,  B01L2400/0445 ,  C12Q1/686 ,  G01N21/00

Abstract: A convective polymerase chain reaction apparatus includes a tube, a temperature control unit, at least one light source and a sensor. The tube includes a cavity used to contain a reaction solution. The reaction solution has a liquid level measured from a bottom of the cavity to a top surface of the reaction solution. The temperature control unit is disposed adjacent to the tube for controlling the temperature of the reaction solution. The at least one light source provides a light beam passing through an incident portion of the tube to excite the reaction solution emitting a fluorescent light. The incident portion is located at a height greater than ½ of a liquid level. The sensor is adjacent to the tube for detecting the excited fluorescence. The light beam has an incident direction forming a non-straight angle with a long axis of the tube.