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    Method for preparing oligonucleotides
    1.
    发明申请
    Method for preparing oligonucleotides 审中-公开
    寡核苷酸的制备方法

    公开(公告)号：US20100069623A1

    公开(公告)日：2010-03-18

    申请号：US12417750

    申请日：2009-04-03

    申请人： Ilaria Adamo ,  Cècile Dueymes ,  Andreas Schönberger ,  Jean-Louis Imbach ,  Albert Meyer ,  Francois Morvan ,  Francoise Debart ,  Jean-Jacques Vasseur ,  Meinolf Lange ,  Fritz Link

    发明人： Ilaria Adamo ,  Cècile Dueymes ,  Andreas Schönberger ,  Jean-Louis Imbach ,  Albert Meyer ,  Francois Morvan ,  Francoise Debart ,  Jean-Jacques Vasseur ,  Meinolf Lange ,  Fritz Link

    IPC分类号： C07H21/04

    CPC分类号： C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  Y02P20/55 ,  Y02P20/582

    摘要： A solution phase synthesis method for preparing an oligonucleotide, wherein at least some of the reagents are solid supported. The method suitable for large-scale synthesis comprises coupling a protected compound with a nucleotide derivative having a protection group in the presence of a solid supported activator to give an elongated oligonucleotide with a P(III)-internucleotide bond; optionally processing the elongated oligonucleotide by capping by reaction with a solid supported capping agent and/or by oxidizing or sulfurizing by reaction of the oligonucleotide with a solid supported oxidizing or sulfurization reagent; and removing the protection group. The coupling may include reacting a 3′-protected compound of formula: with a nucleotide derivative having a 5′-protection group, or reacting a 5′-protected compound of formula with a nucleotide derivative having a 3′-protection group.

    摘要翻译： 一种制备寡核苷酸的溶液相合成方法，其中至少一些试剂是固体负载的。 适用于大规模合成的方法包括在保持固体的活化剂存在下将受保护的化合物与具有保护基团的核苷酸衍生物偶联，得到具有P（III） - 核苷酸键的细长寡核苷酸; 任选地通过与固体支持的封端剂反应进行加帽和/或通过寡核苷酸与固体支持的氧化或硫化试剂的反应进行氧化或硫化来加工细长的寡核苷酸; 并移除保护组。 偶联可以包括使下式的3'-保护的化合物与具有5'-保护基团的核苷酸衍生物反应，或使5受保护的式的化合物与具有3'-保护基团的核苷酸衍生物反应。

    Method for preparing oligonucleotides
    2.
    发明授权
    Method for preparing oligonucleotides 失效
    寡核苷酸的制备方法

    公开(公告)号：US08304532B2

    公开(公告)日：2012-11-06

    申请号：US13115845

    申请日：2011-05-25

    申请人： Ilaria Adamo ,  Cecile Dueymes ,  Andreas Schonberger ,  Jean-Louis Imbach ,  Albert Meyer ,  Francois Morvan ,  Francoise Debart ,  Jean-Jacques Vasseur ,  Meinolf Lange ,  Fritz Link

    发明人： Ilaria Adamo ,  Cecile Dueymes ,  Andreas Schonberger ,  Jean-Louis Imbach ,  Albert Meyer ,  Francois Morvan ,  Francoise Debart ,  Jean-Jacques Vasseur ,  Meinolf Lange ,  Fritz Link

    IPC分类号： C07H21/00

    CPC分类号： C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  Y02P20/55 ,  Y02P20/582

    摘要： A solution phase synthesis method for preparing an oligonucleotide, wherein at least some of the reagents are solid supported. The method suitable for large-scale synthesis comprises coupling a protected compound with a nucleotide derivative having a protection group in the presence of a solid supported activator to give an elongated oligonucleotide with a P(III)-internucleotide bond; optionally processing the elongated oligonucleotide by capping by reaction with a solid supported capping agent and/or by oxidizing or sulfurizing by reaction of the oligonucleotide with a solid supported oxidizing or sulfurization reagent; and removing the protection group. The coupling may include reacting a 3′-protected compound of formula: with a nucleotide derivative having a 5′-protection group, or reacting a 5′-protected compound of formula with a nucleotide derivative having a 3′-protection group.

    摘要翻译： 一种制备寡核苷酸的溶液相合成方法，其中至少一些试剂是固体负载的。 适用于大规模合成的方法包括在保持固体的活化剂存在下将受保护的化合物与具有保护基团的核苷酸衍生物偶联，得到具有P（III） - 核苷酸键的细长寡核苷酸; 任选地通过与固体支持的封端剂反应进行加帽和/或通过寡核苷酸与固体支持的氧化或硫化试剂的反应进行氧化或硫化来加工细长的寡核苷酸; 并移除保护组。 偶联可以包括使下式的3'-保护的化合物与具有5'-保护基团的核苷酸衍生物反应，或使5受保护的式的化合物与具有3'-保护基团的核苷酸衍生物反应。

    Method for preparing oligonucleotides
    3.
    发明申请
    Method for preparing oligonucleotides 审中-公开
    寡核苷酸的制备方法

    公开(公告)号：US20060089494A1

    公开(公告)日：2006-04-27

    申请号：US10522854

    申请日：2003-07-30

    申请人： Ilaria Adamo ,  Cecile Dueymes ,  Andreas Schonberger ,  Jean-Louis Imbach ,  Albert Meyer ,  Francois Morvan ,  Francoise Debart ,  Jean-Jacques Vasseur ,  Meinolf Lange ,  Fritz Link

    发明人： Ilaria Adamo ,  Cecile Dueymes ,  Andreas Schonberger ,  Jean-Louis Imbach ,  Albert Meyer ,  Francois Morvan ,  Francoise Debart ,  Jean-Jacques Vasseur ,  Meinolf Lange ,  Fritz Link

    IPC分类号： C07H21/02 ,  C07H21/04

    CPC分类号： C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  Y02P20/55 ,  Y02P20/582

    摘要： A method for preparing an oligonucleotide comprising the steps of a) providing a 3-protected compound having the formula: wherein B is a heterocyclic base R2 is H, a protected 2-hydroxyl group, F, a protected amino group, an O-alkyl group, an O-substituted alkyl, a substituted alkylamino or a C4′-O2′methylen linkage R3 is OR′3, NHR″3, NR″3R′″3, a 3′-protected nucleotide or a 3′-protected oligonucleotide, R′3 is a hydroxyl protecting group, R″3, R′″3 are independently an amine protecting group, b) reacting said compound with a nucleotide derivative having a 5-proctection group in the presence of a solid supported activator to give an elongated oligonucleotide with a P(III)-internucleotide bond c) optionally processing the elongated oligonucleotide with a P(III)-internucleotide bond by either or both of steps c1) and c2) in any sequence c1) capping preferably by reacting with a solid supported capping agent c2) oxidizing preferably by reacting the oligonucleotide with a solid supported oxidizing reagent d) removing the 5′-protection group.

    摘要翻译： 一种制备寡核苷酸的方法，包括以下步骤：a）提供具有下式的3-保护的化合物：其中B是杂环基R2是H，被保护的2-羟基，F，保护的氨基，O-烷基 基团，O-取代的烷基，取代的烷基氨基或C4'-O2'亚甲基键R3是OR'3，NHR'3，NR'3R“'，3'-保护的核苷酸或3' R'3是羟基保护基，R“3”，“3”独立地是胺保护基，b）使所述化合物与具有5-取代基的核苷酸衍生物在 c）通过任何序列c1）和c2）中任一或两个步骤c1）和c2）任选地用P（III） - 核苷酸键处理细长寡核苷酸 优选通过与固体支持的封端剂c2反应，优选通过使寡核苷酸与固体支持物反应来进行氧化 d）除去5'-保护基。

    Method for the Synthesis of Oligonucleotide Derivatives
    6.
    发明申请
    Method for the Synthesis of Oligonucleotide Derivatives 失效
    寡核苷酸衍生物的合成方法

    公开(公告)号：US20110245478A1

    公开(公告)日：2011-10-06

    申请号：US13081986

    申请日：2011-04-07

    申请人： François Morvan ,  Albert Meyer ,  Jean-Jacques Vasseur ,  Sébastien Vidal ,  Jean-Pierre Cloarec ,  Yann Chevolot ,  Eliane Souteyrand

    发明人： François Morvan ,  Albert Meyer ,  Jean-Jacques Vasseur ,  Sébastien Vidal ,  Jean-Pierre Cloarec ,  Yann Chevolot ,  Eliane Souteyrand

    IPC分类号： C07H21/04

    CPC分类号： C07H21/00 ,  Y10T436/143333

    摘要： Method for the synthesis of nucleotide derivatives wherein molecules of interest are grafted on the oligonucleotide with the help of a “click chemistry” reaction between an azide function on the molecule of interest and an alkyne function on the oligonucleotide, or between an alkyne function on the molecule of interest and an azide function on the oligonucleotide.Intermediate molecules, notably alkyne functionalized oligonucleotides, grafted oligonucleotides, azide functionalized oligonucleotides, oligonucleotide micro arrays containing them and the use of those grafted oligonucleotides for biological investigation and for cell targeting.

    摘要翻译： 合成核苷酸衍生物的方法，其中目的分子在寡核苷酸上接枝，借助于目标分子上的叠氮化物官能团与寡核苷酸上的炔官能团之间的“点击化学”反应，或者在 目标分子和寡核苷酸上的叠氮基功能。 中间分子，特别是炔官能化寡核苷酸，接枝寡核苷酸，叠氮化物官能化寡核苷酸，含有它们的寡核苷酸微阵列以及这些接枝寡核苷酸用于生物学研究和用于细胞靶向的用途。

    Firing mechanism for an automatic firing weapon
    8.
    发明授权
    Firing mechanism for an automatic firing weapon 失效
    自动射击武器的射击机制

    公开(公告)号：US4607563A

    公开(公告)日：1986-08-26

    申请号：US603184

    申请日：1984-04-23

    申请人： Albert Meyer ,  Hans Hofmann ,  Hans Haferli

    发明人： Albert Meyer ,  Hans Hofmann ,  Hans Haferli

    IPC分类号： F41A7/00 ,  F41A19/00 ,  F41A19/03 ,  F41A19/06 ,  F41A19/12 ,  F41D11/02

    CPC分类号： F41A19/12

    摘要： The firing mechanism for an automatic firing weapon comprises a catch pawl or detent by means of which a to-and-fro moving weapon breechblock can be retained in its rear or rearmost position. To prevent damage to the breechblock or the catch pawl it is necessary, upon impact of the breechblock against the catch pawl, for such catch pawl to have been completely pivoted into the path of travel of the breechblock. The release of the catch pawl, which can be pivoted by the action of a spring into the path of travel of the breechblock, is accomplished by a feeler lever. This feeler lever fixedly retains the catch pawl in its breechblock release position by means of a firing or trigger lever. The feeler lever is retained, on the one hand, by a control or cam surface of the breechblock and, on the other hand, by a stop lever in its locking position in which it holds the firing lever in a position preventing the catch pawl from engaging with the breechblock. The stop lever can be pivoted out of its locking position for the feeler lever by a release lever which can be actuated in conjunction with the firing lever.

    摘要翻译： 用于自动射击武器的击发机构包括捕捉棘爪或棘爪，通过该卡爪或棘爪可以将来回移动的武器枪膛保持在其后部或后部位置。 为了防止对后膛块或卡爪的损坏，必须在后膛撞击止动爪的作用下使这种止动爪完全枢转到后膛的行进路径中。 可通过弹簧杆的作用枢转的止动爪释放到后膛的行进路径中，通过一个探杆来实现。 该触发杆通过点火或触发杆将捕捉棘爪固定地保持在其后膛释放位置。 触针杆一方面由后挡块的控制或凸轮表面保持，另一方面由止动杆保持在其锁定位置，在该锁定位置中，止动杆将击发杆保持在防止止动爪的位置 与臀部接触。 止动杆可以通过可与发射杆一起致动的释放杆枢转离开其用于测杆的锁定位置。

    Self-damping circuit
    9.
    发明授权
    Self-damping circuit 失效
    自衰减电路

    公开(公告)号：US3940635A

    公开(公告)日：1976-02-24

    申请号：US501747

    申请日：1974-08-29

    申请人： Albert Meyer

    发明人： Albert Meyer

    IPC分类号： G10H1/26 ,  G10H1/02 ,  H03K17/26

    CPC分类号： G10H1/26 ,  G10H2230/351

    摘要： A Zimbelstern system or sequential bell ringing system in which a sequential counter actuates a set of self-damping circuits in a predetermined repeating sequence, the self-damping circuits each enabling a different set of sinusoidal tone sources in such relative amplitudes and with such relative rise and decay envelopes as to simulate the sounds of sequentially sounding bells when electro-acoustically reproduced.

    摘要翻译： Zimbelstern系统或顺序钟铃系统，其中顺序计数器以预定的重复序列致动一组自消减电路，每个自衰减电路使得能够在这样的相对幅度和相对上升的不同组的正弦音调源 并衰减信封，以便在电声再现时模拟顺序发声的铃声。