公开(公告)号：US20190224676A1

公开(公告)日：2019-07-25

申请号：US16244776

申请日：2019-01-10

申请人： Illumina, Inc. ,  Illumina Cambridge Limited

发明人： Wesley A. COX-MURANAMI ,  Darren Robert SEGALE ,  Oliver Jon MILLER ,  Jennifer Olivia FOLEY ,  Tarun Kumar KHURANA ,  Paul CRIVELLI ,  Gary WATTS

IPC分类号： B01L3/00 ,  G01N1/31

摘要： An apparatus for directing fluid into and out of a fluidic device includes two or more fluid prime channels connected to a fluid inlet of the fluidic device, a flow control valve for each fluid prime channel to control flow between the fluid prime channel and the fluid inlet, one or more outlet channels connected to a fluid outlet of the fluidic device, and a flow control valve for each outlet channel to control flow between the fluid outlet and the associated outlet channel. An apparatus for delivering fluids to a fluid inlet includes a plate that is rotatable about an axis of rotation and a plurality of fluid compartments disposed on the plate, each compartment having a fluid exit port disposed at a common radial distance from the axis of rotation and positioned to align with the fluid inlet as the plate rotates about the axis of rotation.

公开(公告)号：US20230048094A1

公开(公告)日：2023-02-16

申请号：US17981035

申请日：2022-11-04

申请人： ILLUMINA, INC.

发明人： Paul CRIVELLI ,  Cyril DELATTRE

IPC分类号： B01L3/00 ,  G01N35/10

摘要： An apparatus includes a fluid reservoir and a laminate fluidic circuit positioned above the fluid reservoir. The laminate fluidic circuit includes two or more layers laminated together to define a substantially planar substrate and one or more channels defined within the substrate. The laminate fluidic circuit includes a flexible conduit defined by a portion of the substrate encompassing an extent of at least one of the channels that is partially separated or separable from the remainder of the substrate. The flexible conduit is deflectable with respect to the planar substrate toward the fluid reservoir such that the flexible conduit fluidly connects the at least one channel to the fluid reservoir.

公开(公告)号：US20200254450A1

公开(公告)日：2020-08-13

申请号：US16641092

申请日：2019-11-14

申请人： ILLUMINA, INC.

发明人： Paul CRIVELLI ,  Cyril DELATTRE

IPC分类号： B01L3/00 ,  G01N35/10

摘要： An apparatus includes a fluid reservoir and a laminate fluidic circuit positioned above the fluid reservoir. The laminate fluidic circuit includes two or more layers laminated together to define a substantially planar substrate and one or more channels defined within the substrate. The laminate fluidic circuit includes a flexible conduit defined by a portion of the substrate encompassing an extent of at least one of the channels that is partially separated or separable from the remainder of the substrate. The flexible conduit is deflectable with respect to the planar substrate toward the fluid reservoir such that the flexible conduit fluidly connects the at least one channel to the fluid reservoir.

公开(公告)号：US20240319224A1

公开(公告)日：2024-09-26

申请号：US18732450

申请日：2024-06-03

申请人： Illumina, Inc.

发明人： Wesley A. COX-MURANAMI ,  James OSMUS ,  Paul CRIVELLI ,  Bradley Kent DREWS

IPC分类号： G01N35/10

CPC分类号： G01N35/1097

摘要： An apparatus includes a fluidic circuit, a bypass fluidic circuit, a first set of fluid wells, a second set of fluid wells, a first valve, and a second valve. The first valve operatively associated with the first set of fluid wells such that the first selectively fluidly connects any one of the first set of fluid wells to a first valve outlet. The second valve operatively associated with the fluidic circuit, the bypass fluidic circuit, the first valve outlet, and the second set of fluid wells such that the second valve selectively fluidly connects any one of the second set of fluid wells and the first valve outlet to the fluidic circuit or the first valve outlet to the bypass fluidic circuit.

公开(公告)号：US20220323955A1

公开(公告)日：2022-10-13

申请号：US17809604

申请日：2022-06-29

申请人： Illumina, Inc.

发明人： Sz-Chin LIN ,  Jay TAYLOR ,  Minsoung RHEE ,  Jennifer FOLEY ,  Wesley COX-MURANAMI ,  Cyril DELATTRE ,  Tarun KHURANA ,  Paul CRIVELLI

IPC分类号： B01L3/00 ,  G01N21/05

摘要： An example method includes connecting a flow cell to an instrument. The flow cell includes a flow channel including a manifold section having a manifold section swept volume and a detection section having a detection section swept volume. A ratio of the detection section swept volume to manifold section swept volume is at least 10 to 1. A first reagent is pumped through the flow channel. A first chemical reaction is performed between the first reagent and analytes positioned in the detection section. A subsequent reagent is pumped through the flow channel to flush out the remaining reagent. A concentration of at least 99.95 percent of reagent positioned in the detection section is the subsequent reagent, after pumping a total volume of the subsequent reagent through the flow channel that is equal to or less than 2.5 times a total swept volume of the manifold section plus the detection section.

公开(公告)号：US20190336970A1

公开(公告)日：2019-11-07

申请号：US16400629

申请日：2019-05-01

申请人： Illumina, Inc.

发明人： Sz-Chin LIN ,  Jay TAYLOR ,  Minsoung RHEE ,  Jennifer FOLEY ,  Wesley COX-MURANAMI ,  Cyril DELATTRE ,  Tarun KHURANA ,  Paul CRIVELLI

IPC分类号： B01L3/00 ,  G01N21/05

摘要： In one example, a flow cell includes a plurality of inlet ports sized to receive a flow of reagent from one of a plurality of reagents into the flow cell. An outlet port of the flow cell is sized to pass each flow of reagent out of the flow cell. A flow channel of the flow cell is positioned between, and in fluid communication with, each inlet port and the outlet port. The flow channel includes a manifold section and a detection section. The manifold section has a plurality of manifold branches in fluid communication with a common line, wherein each branch is connected to one of each inlet port. The detection section is in fluid communication with the common line and the outlet port. The detection section is operable to perform a plurality of different chemical reactions between the plurality of reagents and analytes positioned in the detection section.

公开(公告)号：US20190351413A1

公开(公告)日：2019-11-21

申请号：US16401805

申请日：2019-05-02

申请人： Illumina, Inc.

发明人： Cyril DELATTRE ,  Minsoung RHEE ,  Jeffrey LIU ,  Wesley COX-MURANAMI ,  Paul CRIVELLI ,  Jennifer FOLEY ,  Darren SEGALE ,  Jay TAYLOR ,  Matthew HAGE ,  Philip PAIK ,  Erik ALLEGOREN ,  David HERTZOG ,  Alex MOROZ-SMIETANA ,  Xiaoxiao MA ,  Tsukasa TAKAHASHI ,  Brandon WESTERBERG

IPC分类号： B01L3/00

摘要： An instrument includes a reagent management system. The reagent management system includes a plurality of reagent wells, each reagent well operable to contain a reagent of a plurality of reagents positioned therein. The reagent management system is operable to select a flow of reagent from one of the plurality of reagents. A flexible connection includes a laminate stack and includes a first flexible channel in fluid communication with the reagent management system. The first flexible channel is operable to route the flow of reagent therethrough. A flow cell includes a flow channel in fluid communication with the first flexible channel. The flow channel is operable to route the flow of reagent over analytes positioned in the flow channel. The flexible connection enables the flow cell to be moved by the instrument relative to a fixed reference point in the instrument.