公开(公告)号：US11835460B2

公开(公告)日：2023-12-05

申请号：US16482638

申请日：2018-01-31

申请人： Illumina, Inc.

发明人： John S. Vieceli ,  Alex Nemiroski ,  Paul Belitz ,  Robert Langlois ,  M. Shane Bowen ,  Danny Yuan Chan ,  Bala Murali K. Venkatesan ,  Hui Han ,  Kevan Samiee ,  Stephen Tanner

IPC分类号： G01N21/64 ,  B01J19/00 ,  C12Q1/6837

CPC分类号： G01N21/6456 ,  B01J19/0046 ,  C12Q1/6837 ,  G01N21/6452 ,  B01J2219/0056 ,  B01J2219/00317 ,  B01J2219/00529 ,  B01J2219/00547 ,  B01J2219/00549 ,  B01J2219/00576 ,  B01J2219/00608 ,  B01J2219/00648 ,  B01J2219/00722 ,  B01L2300/021 ,  G01N2021/6419 ,  G01N2021/6421 ,  H01J2237/24578

摘要： Fiducial markers are provided on patterned arrays of the type that may be used for molecular analysis, such as sequencing. The fiducials may have configurations and layouts that enhance their detection in image or detection data, that facilitate or improve processing, that provide encoding of useful information, and so forth. Examples of the fiducials may include offset layouts that may be useful in detecting the fiducials in different types and approaches in imaging, and that may help to distinguish regions of the array from one another in image data.

公开(公告)号：US20210150715A1

公开(公告)日：2021-05-20

申请号：US17157622

申请日：2021-01-25

申请人： Illumina, Inc.

发明人： Francisco Garcia ,  Klaus Maisinger ,  Stephen Tanner ,  John Moon ,  Tobias Mann ,  Michael Lawrence Parkinson ,  Anthony James Cox ,  Haifang H. Ge

IPC分类号： G06T7/00 ,  G06T7/33 ,  G06T7/246 ,  G06T7/90

摘要： Methods and systems for analysis of image data generated from various reference points. Particularly, the methods and systems provided are useful for real time analysis of image and sequence data generated during DNA sequencing methodologies.

公开(公告)号：US20210310065A1

公开(公告)日：2021-10-07

申请号：US17348212

申请日：2021-06-15

申请人： Illumina, Inc.

发明人： Paul Belitz ,  Stephen Tanner ,  John S. Vieceli ,  Xiaoyu Chen

IPC分类号： C12Q1/6869 ,  G16B30/00

摘要： Methods and systems for analysis of image data generated from various reference points. Particularly, the methods and systems provided are useful for real time analysis of image and sequence data generated during DNA sequencing methodologies.

公开(公告)号：US20200248252A1

公开(公告)日：2020-08-06

申请号：US16482613

申请日：2018-01-31

申请人： Illumina, Inc.

发明人： John S. Vieceli ,  Alex Nemiroski ,  Paul Belitz ,  Robert Langlois ,  M. Shane Bowen ,  Danny Yuan Chan ,  Bala Murali K. Venkatesan ,  Hui Han ,  Kevan Samiee ,  Stephen Tanner

IPC分类号： C12Q1/6874 ,  G01N21/64

摘要： Fiducial markers are provided on patterned arrays of the type that may be used for molecular analysis, such as sequencing. The fiducials may have configurations that enhance their detection in image or detection data, that facilitate or improve processing, that provide encoding of useful information, and so forth. Examples of the fiducials may include a non-closed shape that may encode information, allow for bubbles to escape during manufacture, and provide additional advantages over closed shape fiducials.

公开(公告)号：US20200064266A1

公开(公告)日：2020-02-27

申请号：US16482653

申请日：2018-01-31

申请人： Illumina, Inc.

发明人： John S. Vieceli ,  Alex Nemiroski ,  Paul Belitz ,  Robert Langlois ,  M. Shane Bowen ,  Danny Yuan Chan ,  Bala Murali K. Venkatesan ,  Hui Han ,  Kevan Samiee ,  Stephen Tanner

IPC分类号： G01N21/64

摘要： Fiducial markers are provided on patterned arrays of the type that may be used for molecular analysis, such as sequencing. The fiducials may have configurations that enhance their detection in image or detection data, that facilitate or improve processing, that provide encoding of useful information, and so forth. Examples of the fiducials may include features and materials that are provided on or in the support of the array and that return at least a portion of incident light by reflection. The fiducials may form gratings or other encoding configurations that assist in image processing, alignment, or other aspects of processing of the array.

公开(公告)号：US20230351598A1

公开(公告)日：2023-11-02

申请号：US18303436

申请日：2023-04-19

申请人： Illumina, Inc.

发明人： Francisco Jose Garcia ,  Klaus Maisinger ,  Stephen Tanner ,  John A. Moon ,  Tobias Mann ,  Michael Lawrence Parkinson ,  Anthony James Cox ,  Haifang H. Ge

IPC分类号： G06T7/00 ,  G06T7/33 ,  G06T7/246 ,  G06T7/90

CPC分类号： G06T7/0014 ,  G06T7/33 ,  G06T7/337 ,  G06T7/246 ,  G06T7/248 ,  G06T7/0012 ,  G06T7/90 ,  G06T2207/10024 ,  G06T2207/30024 ,  G06T2207/30072

摘要： Methods and systems for analysis of image data generated from various reference points. Particularly, the methods and systems provided are useful for real time analysis of image and sequence data generated during DNA sequencing methodologies.

公开(公告)号：US11427868B2

公开(公告)日：2022-08-30

申请号：US16482613

申请日：2018-01-31

申请人： Illumina, Inc.

发明人： John S. Vieceli ,  Alex Nemiroski ,  Paul Belitz ,  Robert Langlois ,  M. Shane Bowen ,  Danny Yuan Chan ,  Bala Murali K. Venkatesan ,  Hui Han ,  Kevan Samiee ,  Stephen Tanner

IPC分类号： C12Q1/6874 ,  G01N21/64

摘要： Fiducial markers are provided on patterned arrays of the type that may be used for molecular analysis, such as sequencing. The fiducials may have configurations that enhance their detection in image or detection data, that facilitate or improve processing, that provide encoding of useful information, and so forth. Examples of the fiducials may include a non-closed shape that may encode information, allow for bubbles to escape during manufacture, and provide additional advantages over closed shape fiducials.

公开(公告)号：US11308640B2

公开(公告)日：2022-04-19

申请号：US16706092

申请日：2019-12-06

申请人： ILLUMINA, INC.

发明人： John S. Vieceli ,  Stephen Tanner ,  John A. Moon ,  M. Shane Bowen

IPC分类号： G06T7/73

摘要： A method of registering features in a repeating pattern can include (a) providing an object having a repeating pattern of features and a fiducial; (b) obtaining a target image of the object, wherein the target image includes the repeating pattern of features and the fiducial; (c) comparing the fiducial in the target image to reference data, wherein the reference data includes xy coordinates for a virtual fiducial; and (d) determining locations for the features in the target image based on the comparison of the virtual fiducial in the reference data to the fiducial in the data from the target image. The fiducial can have at least concentric circles that produce three different signal levels. The locations of the features can be determined at a variance of less than 5 μm.

公开(公告)号：US11262307B2

公开(公告)日：2022-03-01

申请号：US16482653

申请日：2018-01-31

申请人： Illumina, Inc.

发明人： John S. Vieceli ,  Alex Nemiroski ,  Paul Belitz ,  Robert Langlois ,  M. Shane Bowen ,  Danny Yuan Chan ,  Bala Murali K. Venkatesan ,  Hui Han ,  Kevan Samiee ,  Stephen Tanner

IPC分类号： G01N21/64 ,  B01L9/00

摘要： Fiducial markers are provided on patterned arrays of the type that may be used for molecular analysis, such as sequencing. The fiducial markers may have configurations that enhance their detection in image or detection data, that facilitate or improve processing, that provide encoding of useful information, and so forth. Examples of the fiducial markers may include features and materials that are provided on or in the support of a patterned array and that return at least a portion of incident light by reflection. The fiducial markers may form gratings or other encoding configurations that assist in image processing, alignment, or other aspects of processing of the patterned array.

公开(公告)号：US20200250851A1

公开(公告)日：2020-08-06

申请号：US16706092

申请日：2019-12-06

申请人： ILLUMINA, INC.

发明人： John S. Vieceli ,  Stephen Tanner ,  John A. Moon ,  M. Shane Bowen

IPC分类号： G06T7/73

摘要： A method of registering features in a repeating pattern can include (a) providing an object having a repeating pattern of features and a fiducial; (b) obtaining a target image of the object, wherein the target image includes the repeating pattern of features and the fiducial; (c) comparing the fiducial in the target image to reference data, wherein the reference data includes xy coordinates for a virtual fiducial; and (d) determining locations for the features in the target image based on the comparison of the virtual fiducial in the reference data to the fiducial in the data from the target image. The fiducial can have at least concentric circles that produce three different signal levels. The locations of the features can be determined at a variance of less than 5 μm.