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    Materials and methods for treating ocular-related disorders
    2.
    发明申请
    Materials and methods for treating ocular-related disorders 审中-公开
    用于治疗眼部相关疾病的材料和方法

    公开(公告)号：US20050220768A1

    公开(公告)日：2005-10-06

    申请号：US11138931

    申请日：2005-05-26

    申请人： Duncan McVey ,  Douglas Brough ,  Imre Kovesdi ,  Lisa Wei

    发明人： Duncan McVey ,  Douglas Brough ,  Imre Kovesdi ,  Lisa Wei

    IPC分类号： A61K20060101 ,  A61K9/00 ,  A61K31/203 ,  A61K31/7088 ,  A61K38/55 ,  A61K45/06 ,  A61K48/00 ,  C12N15/85 ,  C12N15/86 ,  C12N15/861

    CPC分类号： A61K48/005 ,  A61K9/0048 ,  A61K31/203 ,  A61K31/7088 ,  A61K38/57 ,  A61K45/06 ,  A61K48/00 ,  A61K48/0058 ,  A61K48/0075 ,  A61K48/0083 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2799/022 ,  A61K2300/00

    摘要： The invention is directed to a method of delivering a gene product to an animal. The method comprises administering an expression vector comprising a nucleic acid sequence operably linked to a promoter and encoding a gene product, and upregulating transcription of the nucleic acid sequence in the ocular cell. The expression vector can be an adenoviral vector. The invention further provides a method of prophylactically or therapeutically treating an animal for at least one ocular-related disorder. The method comprises contacting an ocular cell with an expression vector comprising a nucleic acid sequence encoding an inhibitor of angiogenesis and/or a neurotrophic agent. In one aspect, the method further comprises upregulating transcription of the nucleic acid sequence. Preferably, if 2×108 adenoviral particles of the inventive method are administered to a mouse, the level of expression of the nucleic acid sequence is not diminished more than ten-fold at 28 days post-administration.

    摘要翻译： 本发明涉及将基因产物递送至动物的方法。 该方法包括施用包含与启动子可操作地连接并编码基因产物的核酸序列的表达载体，以及上调眼细胞中核酸序列的转录。 表达载体可以是腺病毒载体。 本发明还提供了预防性或治疗性地治疗动物至少一种眼部相关疾病的方法。 该方法包括使眼细胞与包含编码血管发生抑制剂和/或神经营养剂的核酸序列的表达载体接触。 在一个方面，该方法还包括上调核酸序列的转录。 优选地，如果向小鼠施用本发明方法的2×10 8个腺病毒颗粒，则在给药后28天，核酸序列的表达水平不会降低10倍以上。

    MATERIALS AND METHODS FOR TREATING OCULAR-RELATED DISORDERS
    3.
    发明申请
    MATERIALS AND METHODS FOR TREATING OCULAR-RELATED DISORDERS 审中-公开
    用于治疗与眼睛有关的疾病的材料和方法

    公开(公告)号：US20070098692A1

    公开(公告)日：2007-05-03

    申请号：US11610364

    申请日：2006-12-13

    申请人： Imre Kovesdi ,  Douglas Brough ,  Lisa Wei ,  Duncan McVey

    发明人： Imre Kovesdi ,  Douglas Brough ,  Lisa Wei ,  Duncan McVey

    IPC分类号： A61K48/00

    CPC分类号： C12N15/86 ,  A61K48/00 ,  A61K48/005 ,  A61K48/0075 ,  C07K14/71 ,  C07K14/811 ,  C12N2710/10343 ,  C12N2799/022

    摘要： The present invention is directed to a method of prophylactically or therapeutically treating an animal for at least one ocular-related disorder, e.g., ocular neovascularization or age-related macular degeneration. The method comprises contacting an ocular cell with an expression vector comprising a nucleic acid sequence encoding an inhibitor of angiogenesis and the same or different nucleic acid sequence encoding a neurotrophic agent. The method also can comprise contacting an ocular cell with different expression vectors, each comprising a nucleic acid sequence encoding an inhibitor of angiogenesis and/or a nucleic acid sequence encoding a neurotrophic agent. In addition, the present invention provides a viral vector comprising a nucleic acid sequence encoding pigment epithelium-derived factor (PEDF) or a therapeutic fragment thereof.

    摘要翻译： 本发明涉及一种预防性或治疗性地治疗动物至少一种与眼部相关的疾病，例如眼睛新生血管形成或年龄相关性黄斑变性的方法。 该方法包括使眼细胞与包含编码血管生成抑制剂的核酸序列和编码神经营养剂的相同或不同核酸序列的表达载体接触。 该方法还可以包括使眼细胞与不同的表达载体接触，每种表达载体包含编码血管生成抑制剂的核酸序列和/或编码神经营养剂的核酸序列。 此外，本发明提供了包含编码色素上皮衍生因子（PEDF）的核酸序列或其治疗片段的病毒载体。

    METHODS OF PROPAGATING MONKEY ADENOVIRAL VECTORS
    6.
    发明申请
    METHODS OF PROPAGATING MONKEY ADENOVIRAL VECTORS 有权
    传播猴子腺病毒载体的方法

    公开(公告)号：US20120225470A1

    公开(公告)日：2012-09-06

    申请号：US13508659

    申请日：2010-11-09

    申请人： Jason Gall ,  Douglas Brough ,  Christoph Kahl ,  Duncan McVey

    发明人： Jason Gall ,  Douglas Brough ,  Christoph Kahl ,  Duncan McVey

    IPC分类号： C12N7/00

    CPC分类号： C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10332 ,  C12N2710/10343 ,  C12N2710/10352

    摘要： The invention provides methods for propagating a monkey adenovirus in a cell including a human cell, comprising one or more gene products isolated from a human adenovirus. Also provided are methods for propagating wherein the monkey adenovirus comprises a nucleic acid sequence encoding a human adenovirus gene product. The invention further provides a monkey adenovirus. including a replication-deficient monkey adenovirus, obtained by such propagation methods.

    摘要翻译： 本发明提供了在包括人细胞的细胞中繁殖猴腺病毒的方法，其包含从人腺病毒分离的一种或多种基因产物。 还提供了用于繁殖的方法，其中猴腺病毒包含编码人腺病毒基因产物的核酸序列。 本发明进一步提供猴腺病毒。 包括通过这种繁殖方法获得的复制缺陷型猴腺病毒。

    Methods of gene therapy for treating disorders of the ear by adminstering a vector encoding an antonal-associated factor
    7.
    发明申请
    Methods of gene therapy for treating disorders of the ear by adminstering a vector encoding an antonal-associated factor 审中-公开
    通过管理编码冠状相关因子的载体来治疗耳朵疾病的基因治疗方法

    公开(公告)号：US20070141029A1

    公开(公告)日：2007-06-21

    申请号：US10586072

    申请日：2004-02-19

    申请人： Douglas Brough

    发明人： Douglas Brough

    IPC分类号： A61K48/00 ,  C12N15/861

    CPC分类号： A61K38/17 ,  A61K38/00 ,  A61K48/005 ,  A61P27/00 ,  C07K14/4702 ,  C12N7/00 ,  C12N15/86 ,  C12N2501/60 ,  C12N2710/10343

    摘要： The invention is directed to a method of changing the sensory perception of an animal. The method comprises administering an expression vector comprising a nucleic acid sequence encoding an atonal-associated factor, which is expressed to produce the atonal-associated factor resulting in generation of hair cells that allow perception of stimuli in the inner ear. Also provided is a method of generating a hair cell in differentiated sensory epitheliain vivo. The method comprises contacting differentiated sensory epithelial cells with an adenoviral vector (a) deficient in one or more replication-essential gene functions of the E1 region and E4 region, (b) comprising a spacer in the E4 region, and (c) comprising a nucleic acid sequence encoding an atonal-associated factor. The nucleic acid sequence is expressed to produce the atonal-associated factor such that a hair cell is generated. An adenoviral vector encoding an atonal-associated factor also is provided.

    摘要翻译： 本发明涉及一种改变动物的感觉知觉的方法。 所述方法包括施用包含编码无关联因子的核酸序列的表达载体，所述核酸序列被表达以产生导致产生能够感知内耳中的刺激的毛细胞的无关性因子。 还提供了在分化感觉上皮细胞体内产生毛细胞的方法。 该方法包括使分化的感觉上皮细胞与E1区和E4区的一个或多个复制必需基因功能缺陷的腺病毒载体（a）接触，（b）在E4区中包含间隔基，和（c）包含 编码无关联因子的核酸序列。 表达核酸序列以产生无创因子，从而产生毛细胞。 还提供了编码无关联因子的腺病毒载体。

    Adenoviral vector-based vaccines
    8.
    发明申请
    Adenoviral vector-based vaccines 审中-公开
    基于腺病毒载体的疫苗

    公开(公告)号：US20060286121A1

    公开(公告)日：2006-12-21

    申请号：US11337866

    申请日：2006-01-23

    申请人： Jason Gall ,  Thomas Wickham ,  William Enright ,  Douglas Brough ,  Mohammed Zuber ,  C. King ,  Gary Nabel ,  Cheng Cheng

    发明人： Jason Gall ,  Thomas Wickham ,  William Enright ,  Douglas Brough ,  Mohammed Zuber ,  C. King ,  Gary Nabel ,  Cheng Cheng

    IPC分类号： A61K39/12 ,  A61K39/395

    CPC分类号： C12N15/86 ,  A61K48/00 ,  A61K2039/5256 ,  C07K2319/00 ,  C12N2710/10343 ,  C12N2710/10345

    摘要： The invention provides a method of inducing an immune response in a mammal. The method comprises administering to the mammal a non-subgroup C adenoviral vector comprising an adenoviral fiber protein having an amino acid sequence comprising about 80% or more identity to an amino acid sequence encoding a subgroup C adenoviral fiber protein. The adenoviral vector further comprises a nucleic acid sequence encoding an antigen which is expressed in the mammal to induce an immune response. The invention further comprises a method of producing an adenoviral vector, and a composition comprising a serotype 41 or a serotype 35 adenoviral vector and a carrier. The invention also provides an adenoviral vector comprising a nucleic acid sequence encoding an adenoviral pIX protein operably linked to a heterologous expression control sequence, as well as a method of enhancing the stability and/or packaging capacity of an adenoviral vector.

    摘要翻译： 本发明提供在哺乳动物中诱导免疫应答的方法。 该方法包括向哺乳动物施用非亚组C腺病毒载体，其包含具有与编码亚组C腺病毒纤维蛋白的氨基酸序列具有约80％或更高同一性的氨基酸序列的腺病毒纤维蛋白。 腺病毒载体还包含编码在哺乳动物中表达以诱导免疫应答的抗原的核酸序列。 本发明还包括产生腺病毒载体的方法和包含血清型41或血清型35腺病毒载体和载体的组合物。 本发明还提供了一种腺病毒载体，其包含编码与异源表达控制序列可操作地连接的腺病毒pIX蛋白的核酸序列，以及增强腺病毒载体的稳定性和/或包装能力的方法。