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公开(公告)号：US08268323B2
公开(公告)日：2012-09-18
申请号：US13232775
申请日：2011-09-14
申请人： Peter Kwong , John Mascola , Gary Nabel , Richard Wyatt , Barna Dey , Ling Xu , Tongqing Zhou , Joseph Sodroski , Wen Yuan , Shi-Hua Xiang
发明人： Peter Kwong , John Mascola , Gary Nabel , Richard Wyatt , Barna Dey , Ling Xu , Tongqing Zhou , Joseph Sodroski , Wen Yuan , Shi-Hua Xiang
IPC分类号： A61K39/21 , A61K39/385 , A61K9/127 , G01N33/53
CPC分类号： C07K14/005 , A61K39/12 , A61K39/21 , A61K2039/53 , A61K2039/54 , A61K2039/545 , C07K14/70514 , C12N2740/15022 , C12N2740/16122 , C12N2740/16134
摘要： Stabilized forms of gp120 polypeptide, nucleic acids encoding these stabilized forms, vectors comprising these nucleic acids, and methods of using these polypeptides, nucleic acids, vectors and host cells are disclosed. Crystal structures and computer systems including atomic coordinates for stabilized forms of gp120, and gp120 with an extended V3 loop, and methods of using these structures and computer systems are also disclosed.
摘要翻译： 公开了稳定形式的gp120多肽，编码这些稳定形式的核酸，包含这些核酸的载体，以及使用这些多肽，核酸，载体和宿主细胞的方法。 还公开了包括gp120的稳定形式的原子坐标和具有扩展的V3循环的gp120的晶体结构和计算机系统，以及使用这些结构和计算机系统的方法。
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2.发明授权Conformationally stabilized HIV envelope immunogens and triggering HIV-I envelope to reveal cryptic V3-loop epitopes 有权构象稳定的艾滋病毒包膜免疫原，并引发HIV-1信封揭示神秘的V3环表位公开(公告)号：US08044185B2
公开(公告)日：2011-10-25
申请号：US12065894
申请日：2006-09-06
申请人： Peter Kwong , John Mascola , Gary Nabel , Richard Wyatt , Barna Dey , Ling Xu , Tongqing Zhou , Chih-Chin Huang , Joseph Sodroski , Wen Yuan , Shi-Hua Xiang
发明人： Peter Kwong , John Mascola , Gary Nabel , Richard Wyatt , Barna Dey , Ling Xu , Tongqing Zhou , Chih-Chin Huang , Joseph Sodroski , Wen Yuan , Shi-Hua Xiang
CPC分类号： C07K14/005 , A61K39/12 , A61K39/21 , A61K2039/53 , A61K2039/54 , A61K2039/545 , C07K14/70514 , C12N2740/15022 , C12N2740/16122 , C12N2740/16134
摘要： Stabilized forms of gp120 polypeptide, nucleic acids encoding these stabilized forms, vectors comprising these nucleic acids, and methods of using these polypeptides, nucleic acids, vectors and host cells are disclosed. Crystal structures and computer systems including atomic coordinates for stabilized forms of gp120, and gp120 with an extended V3 loop, and methods of using these structures and computer systems are also disclosed.
摘要翻译： 公开了稳定形式的gp120多肽，编码这些稳定形式的核酸，包含这些核酸的载体，以及使用这些多肽，核酸，载体和宿主细胞的方法。 还公开了包括gp120的稳定形式的原子坐标和具有扩展的V3循环的gp120的晶体结构和计算机系统，以及使用这些结构和计算机系统的方法。
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公开(公告)号：US20090155853A1
公开(公告)日：2009-06-18
申请号：US12092811
申请日：2006-11-20
申请人： Gary Nabel , Wataru Akahata
发明人： Gary Nabel , Wataru Akahata
CPC分类号： C12N15/87 , A61K48/00 , C07K14/005 , C07K2319/74 , C07K2319/80 , C12N2710/10322
摘要： The invention relates to fusion proteins useful in delivering a targeted nucleic acid to a target cell, comprising a gene delivery fusion protein (GDFP), said GDFP comprising a nucleic acid binding domain (NBD) that binds to the targeted nucleic acid, fused to a gene delivery domain (GDD) that mediates delivery of the targeted nucleic acid to the target cell, wherein said GDD comprises one or more components that facilitate delivery of a targeted nucleic acid to a target cell, and wherein one of said components is a transport/localization component and wherein said transport/localization component is an adenovirus protein V or derivative thereof that retains protein V activity, and related methods of making and using thereof.
摘要翻译： 本发明涉及可用于将靶向核酸递送至靶细胞的融合蛋白，其包含基因递送融合蛋白（GDFP），所述GDFP包含与靶向核酸结合的核酸结合结构域（NBD），其与 介导靶向核酸向靶细胞递送的基因递送结构域（GDD），其中所述GDD包含有利于将靶向核酸递送至靶细胞的一种或多种组分，并且其中所述组分之一是转运/ 定位组分，其中所述转运/定位组分是保留蛋白V活性的腺病毒蛋白V或其衍生物，以及其制备和使用的相关方法。
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4.发明申请公开(公告)号：US20090110690A1
公开(公告)日：2009-04-30
申请号：US12065883
申请日：2006-09-06
申请人： Peter D. Kwong , Tongqing Zhou , Ling Xu , Gary Nabel , Barna Dey , Richard Wyatt , James Arthos , Joseph Sodroski , Shi-Hua Xiang , Dennis Burton
发明人： Peter D. Kwong , Tongqing Zhou , Ling Xu , Gary Nabel , Barna Dey , Richard Wyatt , James Arthos , Joseph Sodroski , Shi-Hua Xiang , Dennis Burton
IPC分类号： A61K39/21 , C07K14/16 , C07H21/04 , C12N15/63 , C12N5/06 , A61P31/18 , C12N5/22 , A61K38/16 , A61K31/7052
CPC分类号： C07K14/005 , A61K39/12 , A61K39/21 , A61K2039/53 , A61K2039/54 , A61K2039/545 , C07K14/70514 , C12N2740/15022 , C12N2740/16122 , C12N2740/16134
摘要： The present disclosure relates to stabilized forms of the HIV gp120 envelope protein in complex with the broadly neutralizing CD4-binding site antibody b12, to crystalline forms of the stabilized forms of the HIV gp120 envelope protein in complex with the broadly neutralizing CD4-binding site antibody b12, and to the high resolution structure obtained from these crystals by X-ray diffraction methods. Methods for identifying immunogenic polypeptides based on these structures are also disclosed.
摘要翻译： 本公开涉及与广泛中和的CD4结合位点抗体b12复合的HIV gp120包膜蛋白的稳定形式，与广泛中和的CD4结合位点抗体复合的HIV gp120包膜蛋白的稳定形式的结晶形式 b12，以及通过X射线衍射法从这些晶体获得的高分辨率结构。 还公开了基于这些结构鉴定免疫原性多肽的方法。
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公开(公告)号：US20050180993A1
公开(公告)日：2005-08-18
申请号：US11031921
申请日：2005-01-07
申请人： Gary Nabel , Yue Huang
发明人： Gary Nabel , Yue Huang
IPC分类号： G01N33/50 , A61K39/00 , A61K39/12 , A61K39/17 , A61K45/00 , A61P31/12 , C07K14/08 , C12Q1/02 , C12Q1/18 , C12Q1/34 , C12Q1/70 , G01N33/15 , G01N33/569
CPC分类号： G01N33/56983 , C12Q1/18
摘要： The present invention relates to assays for the identification of compounds that inhibit assembly of NP, VP35, and VP24, or inhibit the glycosylation of NP, required for nucleocapsid formation, for use as anti-viral agents. The invention also relates to assays for the identification of compounds that block glycosylation of proteins having a glycosylation domain that is substantially homologous to a glycosylation domain of NP required for polymerization. The invention further relates to pseudoparticles for presentation of antigens or antigenic epitopes for immunogenic or vaccination purposes.
摘要翻译： 本发明涉及用于鉴定抑制NP，VP35和VP24的组装或抑制核衣壳形成所需的NP的糖基化作为抗病毒剂的化合物的测定。 本发明还涉及用于鉴定封闭糖基化的化合物的测定法，其具有与聚合所需的NP的糖基化结构域基本上同源的糖基化结构域。 本发明还涉及用于呈递用于免疫原性或疫苗接种目的的抗原或抗原表位的伪颗粒。
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6.发明授权Nucleic acids encoding modified Ebola virus glycoproteins with diminished cytotoxicity while retaining native antigenic structures 有权编码改良埃博拉病毒糖蛋白的核酸，同时保留天然抗原结构，减少细胞毒性公开(公告)号：US09012618B2
公开(公告)日：2015-04-21
申请号：US13349917
申请日：2012-01-13
申请人： Nancy Sullivan , Bimal Chakrabarti , Zhi-Yong Yang , Maria Grazia Pau , Jaap Goudsmit , Gary Nabel
发明人： Nancy Sullivan , Bimal Chakrabarti , Zhi-Yong Yang , Maria Grazia Pau , Jaap Goudsmit , Gary Nabel
CPC分类号： C12N15/86 , A61K39/12 , A61K2039/5156 , A61K2039/5254 , A61K2039/5256 , A61K2039/53 , C07K14/005 , C12N2710/10343 , C12N2740/17022 , C12N2760/14022 , C12N2760/14134 , C12N2840/44
摘要： The invention is related to a nucleic acid molecule comprising a polynucleotide encoding a modified filovirus glycoprotein (GP) having at least one amino acid change located in a relatively conserved region of said GP that decreases in vitro cytotoxicity and retains immunogenicity when compared to in vitro cytotoxicity and immunogenicity of a wild type filovirus GP, and related modified filovirus GPs, plasmid DNAs, recombinant viruses, adenoviruses, pharmaceutical compositions, vaccine compositions, antibodies that are specifically reactive with the modified filovirus GPs, and related methods of making and using the same.
摘要翻译： 本发明涉及包含编码经修饰的病毒糖蛋白（GP）的多核苷酸的核酸分子，其具有位于所述GP的相对保守区域中的至少一个氨基酸改变，其降低体外细胞毒性并且与体外细胞毒性相比保留免疫原性 野生型病毒GP和相关修饰的病毒GP，质粒DNA，重组病毒，腺病毒，药物组合物，疫苗组合物，与修饰的病毒GP特异性反应的抗体的免疫原性及其制备和使用的相关方法。
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公开(公告)号：US20120328641A1
公开(公告)日：2012-12-27
申请号：US13585700
申请日：2012-08-14
申请人： Peter Kwong , John Mascola , Gary Nabel , Richard Wyatt , Barna Dey , Ling Xu , Tongqing Zhou , Joseph Sodroski , Wen Yuan , Shi-Hua Xiang
发明人： Peter Kwong , John Mascola , Gary Nabel , Richard Wyatt , Barna Dey , Ling Xu , Tongqing Zhou , Joseph Sodroski , Wen Yuan , Shi-Hua Xiang
CPC分类号： C07K14/005 , A61K39/12 , A61K39/21 , A61K2039/53 , A61K2039/54 , A61K2039/545 , C07K14/70514 , C12N2740/15022 , C12N2740/16122 , C12N2740/16134
摘要： Stabilized forms of gp120 polypeptide, nucleic acids encoding these stabilized forms, vectors comprising these nucleic acids, and methods of using these polypeptides, nucleic acids, vectors and host cells are disclosed. Crystal structures and computer systems including atomic coordinates for stabilized forms of gp120, and gp120 with an extended V3 loop, and methods of using these structures and computer systems are also disclosed.
摘要翻译： 公开了稳定形式的gp120多肽，编码这些稳定形式的核酸，包含这些核酸的载体，以及使用这些多肽，核酸，载体和宿主细胞的方法。 还公开了包括gp120的稳定形式的原子坐标和具有扩展的V3循环的gp120的晶体结构和计算机系统，以及使用这些结构和计算机系统的方法。
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公开(公告)号：US20060239975A1
公开(公告)日：2006-10-26
申请号：US11475827
申请日：2006-06-27
申请人： Gary Nabel , Zhi-yong Yang , Elizabeth Nabel
发明人： Gary Nabel , Zhi-yong Yang , Elizabeth Nabel
CPC分类号： C12N15/86 , A61K9/0019 , A61K31/70 , A61K31/711 , A61K38/1709 , A61K38/1774 , A61K38/45 , A61K48/005 , C12N2710/10343 , A61K2300/00
摘要： The p21 gene encodes a cyclin dependent kinase inhibitor which affects cell cycle progression, but the role of this gene product in altering tumor growth has not been established. The present inventors have now discovered that the growth of malignant cells in vivo is inhibited by expression of p21. Expression of p21 resulted in an accumulation of cells in G0/G1, alteration in morphology, and cell differentiation.
摘要翻译： p21基因编码影响细胞周期进程的细胞周期蛋白依赖性激酶抑制剂，但该基因产物在改变肿瘤生长中的作用尚未确定。 本发明人现已发现，通过p21的表达抑制体内恶性细胞的生长。 p21的表达导致细胞在G0 / G1 / 1中的积累，形态的改变和细胞分化。
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公开(公告)号：US20060013832A1
公开(公告)日：2006-01-19
申请号：US11029188
申请日：2005-01-04
申请人： Carl June , Craig Thompson , Gary Nabel , Gary Gray , Paul Rennert
发明人： Carl June , Craig Thompson , Gary Nabel , Gary Gray , Paul Rennert
CPC分类号： C12N5/0636 , A61K35/17 , A61K38/00 , A61K39/02 , A61K39/12 , A61K48/00 , A61K2039/505 , A61K2039/507 , A61K2039/5158 , C07K14/705 , C07K14/70521 , C07K14/70532 , C07K16/00 , C07K16/2806 , C07K16/2809 , C07K16/2812 , C07K16/2815 , C07K16/2818 , C07K16/2833 , C07K16/2866 , C07K16/289 , C07K16/2896 , C07K2317/24 , C07K2317/34 , C07K2317/54 , C07K2317/55 , C07K2317/73 , C07K2317/74 , C07K2319/00 , C07K2319/30 , C12N2501/51 , C12N2501/515 , C12N2501/599 , C12N2710/00088 , C12N2740/16011
摘要： Methods for inducing a population of T cells to proliferate by activating the population of T cells and stimulating an accessory molecule on the surface of the T cells with a ligand which binds the accessory molecule are described. T cell proliferation occurs in the absence of exogenous growth factors or accessory cells. T cell activation is accomplished by stimulating the T cell receptor (TCR)/CD3 complex or the CD2 surface protein. To induce proliferation of an activated population T cells, an accessory molecule on the surface of the T cells, such as CD28, is stimulated with a ligand which binds the accessory molecule. The T cell population expanded by the method of the invention can be genetically transduced and used for immunotherapy or can be used in methods of diagnosis.
摘要翻译： 描述了通过激活T细胞群并用结合附属分子的配体刺激T细胞表面上的辅助分子来诱导T细胞群体增殖的方法。 T细胞增殖发生在没有外源生长因子或附属细胞的情况下。 通过刺激T细胞受体（TCR）/ CD3复合物或CD2表面蛋白来完成T细胞活化。 为了诱导活化的群体T细胞的增殖，T细胞表面上的辅助分子如CD28被与配合分子结合的配体刺激。 通过本发明的方法扩增的T细胞群体可以被遗传转导并用于免疫治疗，或可用于诊断方法。
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10.发明申请Targeting gene transfer vectors to certain cell types by pseudotyping with viral glycoprotein 审中-公开通过用病毒糖蛋白假型分型将基因转移载体靶向某些细胞类型公开(公告)号：US20050130129A1
公开(公告)日：2005-06-16
申请号：US10860878
申请日：2004-06-03
申请人： Gary Nabel , Rafael Delgado , Zhi-yong Yang
发明人： Gary Nabel , Rafael Delgado , Zhi-yong Yang
IPC分类号： A61K48/00 , C07K14/08 , C12N15/867 , C12N15/88 , C12Q1/70 , C07K14/005 , C12N7/00 , C12Q1/68
CPC分类号： C12N15/86 , A61K48/00 , C07K14/005 , C12N15/88 , C12N2740/13022 , C12N2740/13043 , C12N2740/13045 , C12N2760/14122 , C12N2810/60 , C12N2840/203
摘要： The present invention provides compositions and methods for targeting gene transfer vectors to certain cell types by pseudotyping with a transmembrane form of viral glycoprotein, such as that from Ebola virus. The methods comprise the step of administering to a cell population a gene to be transferred operatively linked to an appropriate transfer vehicle, wherein the transfer vehicle is associated with a transmembrane form of viral glycoprotein.
摘要翻译： 本发明提供了通过用跨膜形式的病毒糖蛋白（例如来自埃博拉病毒的病毒糖蛋白）的假型分型来将基因转移载体靶向某些细胞类型的组合物和方法。 所述方法包括向细胞群体施用可转移的基因可操作地连接到合适的转运载体的步骤，其中所述转运载体与跨膜形式的病毒糖蛋白缔合。
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