公开(公告)号：US20200095557A1

公开(公告)日：2020-03-26

申请号：US16580954

申请日：2019-09-24

Applicant: Indiana University Research and Technology Corporation

Inventor： Nicanor I. Moldovan ,  Mervin C. Yoder ,  Nutan Prasain

IPC: C12N5/071 ,  C12N5/00 ,  C12N5/077

Abstract: Co-culture of isolated human induced pluripotent stem cell (iPSC)-derived endothelial and smooth-muscle progenitor cells results in the formation of cellular spheroids having capillary-like structures (referred to herein as ‘capillary fragments’) at their core. Bioprinting of these spheroids into scaffold-free tissue constructs facilitates the development of microvasculature within the engineered tissue. Methods of using these bioprinted engineered tissues for cell therapy are also disclosed.

公开(公告)号：US10563175B2

公开(公告)日：2020-02-18

申请号：US15124280

申请日：2015-03-11

Applicant: Indiana University Research and Technology Corporation

Inventor： Mervin Yoder ,  Nutan Prasain

IPC: C12N5/071 ,  A61K35/44 ,  G01N33/50

Abstract: The present disclosure relates generally to methods and compositions useful in cell and tissue biology and therapeutics. In particular, an in vitro method for differentiating pluripotent cells into endothelial colony forming cell-like cells (ECFC-like cells) is provided. A purified human cell population of NRP-1+CD31+ ECFC-like cells is provided, wherein at least some of the cells in the population have a high proliferation potential. Therapeutic and test agent screening methods for using the cell populations of the present disclosure are provided.

公开(公告)号：US11739293B2

公开(公告)日：2023-08-29

申请号：US16323722

申请日：2017-08-04

Applicant: Indiana University Research and Technology Corporation

Inventor： Mervin C. Yoder ,  Nutan Prasain

IPC: C12N5/0735 ,  C12N5/071 ,  A61K35/12

CPC classification number: C12N5/0606 ,  A61K35/12 ,  C12N5/069 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/165 ,  C12N2501/65 ,  C12N2506/02 ,  C12N2506/03 ,  C12N2506/45 ,  C12N2533/54

Abstract: The present disclosure relates generally to methods and compositions useful in cell and tissue biology and therapeutics. In particular, an in vitro method for differentiating pluripotent stem cells into KDR+NCAM+APLNR+ mesoderm cells and/or SSEA5−KDR+NCAM+APLNR+ mesoderm cells is provided. The disclosed mesoderm cells may be used to generate blood vessels in vivo and/or further differentiated in vitro into endothelial colony forming cell-like cells (ECFC-like cells). Purified human cell populations of KDR+NCAM+APLNR+ mesoderm cells and ECFC-like cells are provided. Test agent screening and therapeutic methods for using the cell populations of the present disclosure are provided.

公开(公告)号：US20190211304A1

公开(公告)日：2019-07-11

申请号：US16323722

申请日：2017-08-04

Applicant: Indiana University Research and Technology Corporation

Inventor： Mervin C. Yoder ,  Nutan Prasain

IPC: C12N5/0735 ,  A61K35/12

CPC classification number: C12N5/0606 ,  A61K35/12 ,  C12N5/069 ,  C12N2501/115 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/165 ,  C12N2501/65 ,  C12N2506/02 ,  C12N2506/03 ,  C12N2506/45 ,  C12N2533/54

Abstract: The present disclosure relates generally to methods and compositions useful in cell and tissue biology and therapeutics. In particular, an in vitro method for differentiating pluripotent stem cells into KDR+NCAM+APLNR+ mesoderm cells and/or SSEA5−KDR+NCAM+APLNR+ mesoderm cells is provided. The disclosed mesoderm cells may be used to generate blood vessels in vivo and/or further differentiated in vitro into endothelial colony forming cell-like cells (ECFC-like cells). Purified human cell populations of KDR+NCAM+APLNR+mesoderm cells and ECFC-like cells are provided. Test agent screening and therapeutic methods for using the cell populations of the present disclosure are provided.

公开(公告)号：US20170022476A1

公开(公告)日：2017-01-26

申请号：US15124280

申请日：2015-03-11

Applicant: Indiana University Research and Technology Corporation

Inventor： Mervin Yoder ,  Nutan Prasain

IPC: C12N5/071 ,  G01N33/50 ,  A61K35/44

Abstract: The present disclosure relates generally to methods and compositions useful in cell and tissue biology and therapeutics. In particular, an in vitro method for differentiating pluripotent cells into endothelial colony forming cell-like cells (ECFC-like cells) is provided. A purified human cell population of NRP-1+CD31+ ECFC-like cells is provided, wherein at least some of the cells in the population have a high proliferation potential. Therapeutic and test agent screening methods for using the cell populations of the present disclosure are provided.

Abstract translation: 本公开一般涉及可用于细胞和组织生物学和治疗学的方法和组合物。 特别地，提供了将多能细胞分化成内皮细胞集落形成细胞样细胞（ECFC样细胞）的体外方法。 提供NRP-1 + CD31 + ECFC样细胞的纯化人细胞群，其中群体中的至少一些细胞具有高增殖潜力。 提供了使用本公开的细胞群体的治疗和测试剂筛选方法。