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公开(公告)号：US6139798A
公开(公告)日：2000-10-31
申请号：US843296
申请日：1997-04-14
申请人： Ingo Klimant , Hellfried Karpf , Otto S. Wolfbeis
发明人： Ingo Klimant , Hellfried Karpf , Otto S. Wolfbeis
CPC分类号： G01N31/225 , G01N31/223 , Y10T436/207497
摘要： The sensor membrane of an optical sensor for detection of O.sub.2, H.sub.2 O.sub.2, SO.sub.2 or halogenated hydrocarbons in a sample contains an indicator substance that is homogeneously immobilized in the polymer matrix of the sensor membrane and is, at least indirectly, in contact with the sample, changing at least one of its optical properties upon a change of the parameter to be measured. The indicator substance contains an inorganic salt of a transition metal complex with .alpha.-diimine ligands. The indicator substance is homogeneously distributed in the polymer matrix, which essentially consists of at least one substance belonging to the group of cellulose derivatives, polystyrenes, polytetrahydrofuranes, or their respective derivatives.
摘要翻译： 用于检测样品中的O 2，H 2 O 2，SO 2或卤代烃的光学传感器的传感器膜包含均匀地固定在传感器膜的聚合物基质中的指示物质，并且至少间接地与样品接触， 在要测量的参数的变化时改变其光学特性中的至少一个。 指示物质含有与α-二亚胺配体的过渡金属络合物的无机盐。 指示剂物质均匀分布在聚合物基质中，其基本上由属于纤维素衍生物，聚苯乙烯，聚四氢呋喃或它们各自的衍生物的至少一种物质组成。
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公开(公告)号：US5942189A
公开(公告)日：1999-08-24
申请号：US950373
申请日：1997-10-14
CPC分类号： G01N31/22 , G01N31/221
摘要： In a sensor layer for quantitative determination of at least one chemical component in a gaseous or liquid sample medium containing a chromophore which is directly or indirectly responsive to the component being determined by changing its absorption spectrum, and a luminophore which is not responsive to the component being determined, where there is an at least partial overlap between the emission spectrum of the luminophore and the absorption spectrum of the chromophore, and where the energy transfer between luminophore and chromophore produces a measurable change in at least one luminescence characteristic of the luminophore, the luminophore L and the chromophore .GAMMA. are ionic substances with differing electrical charges, which are incorporated in a matrix material that is permeable to the chemical component being determined.
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3.发明授权Luminescence-optical method and sensor layer for quantitative determination of at least one chemical component of a gaseous or liquid sample 有权用于定量测定气态或液体样品中至少一种化学成分的发光光学方法和传感器层
公开(公告)号：US6046055A
公开(公告)日：2000-04-04
申请号：US280073
申请日：1999-03-29
CPC分类号： G01N31/22 , G01N31/221
摘要： In a sensor layer for quantitative determination of at least one chemical component in a gaseous or liquid sample medium containing a chromophore which is directly or indirectly responsive to the component being determined by changing its absorption spectrum, and a luminophore which is not responsive to the component being determined, where there is an at least partial overlap between the emission spectrum of the luminophore and the absorption spectrum of the chromophore, and where the energy transfer between luminophore and chromophore produces a measurable change in at least one luminescence characteristic of the luminophore, the luminophore L and the chromophore .GAMMA. are ionic substances with differing electrical charges, which are incorporated in a matrix material that is permeable to the chemical component being determined.
摘要翻译： 在用于定量测定含有发色团的气态或液体样品介质中的至少一种化学成分的传感器层中，所述发色团直接或间接地响应于通过改变其吸收光谱确定的组分，以及不响应于该组分的发光体 在发光体的发射光谱和发色团的吸收光谱之间存在至少部分重叠的地方，以及在发光体和发色团之间的能量转移在发光体的至少一个发光特性中产生可测量的变化的地方， 发光体L和发色团GAMMA是具有不同电荷的离子物质，其被掺入可测定化学成分的基质材料中。
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4.发明申请公开(公告)号：US20130280751A1
公开(公告)日：2013-10-24
申请号：US13879933
申请日：2010-10-22
IPC分类号： G01N21/64
CPC分类号： G01N21/6486 , B82Y30/00 , G01N33/5005 , G01N33/5008 , G01N33/587 , G01N33/84
摘要： The invention is based on the use of photoluminescent probes for intracellular sensing of oxygen, especially assaying intracellular oxygen concentration. The photoluminescent probe comprises a suspension of polymeric particles having an average diameter in the 20 nm to 100 nm range, formed from an amphiphilic cationic co-polymer which is oriented in the formed particle to provide a hydrophobic core and a hydrophilic shell. The probe includes a hydrophobic oxygen-sensitive photoluminescent dye such as Pt-tetrakis(pentafluorophenyl)porphine, PtPFPP embedded in the hydrophobic core of the particle, and the co-polymer includes quaternary ammonium groups which provide hydrophilic and cationic character to the particle shell. The photoluminescent probe, which in use is provided in the form of an aqueous suspension of probe, is incubated with live mammalian cells in a suitable growth medium for a period of time to allow the probe particles passively load into the cells. Oxygen can then be sensed by detecting a photoluminescent signal of the photoluminescent probe, which can be correlated with oxygen status, for example oxygen concentration or changes in oxygen concentration/levels, using existing techniques.
摘要翻译： 本发明基于光致发光探针用于细胞内感测氧，特别是测定细胞内氧浓度。 光致发光探针包含平均直径在20nm至100nm范围内的聚合物颗粒的悬浮液，其由在形成的颗粒中定向以提供疏水性芯和亲水壳的两亲性阳离子共聚物形成。 探针包括疏水性氧敏感性光致发光染料如Pt-四（五氟苯基）卟吩，嵌入颗粒的疏水核心中的PtPFPP，共聚物包括为颗粒壳提供亲水和阳离子特征的季铵基团。 将使用中的探针的水溶液悬浮液形式提供的光致发光探针与活的哺乳动物细胞在合适的生长培养基中温育一段时间，以使探针颗粒被动地载入细胞。 然后可以使用现有技术通过检测光致发光探针的光致发光信号来感测氧，其可以与氧状态相关，例如氧浓度或氧浓度/水平的变化。
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公开(公告)号：US20090004751A1
公开(公告)日：2009-01-01
申请号：US12038583
申请日：2008-02-27
IPC分类号： G01N21/75
CPC分类号： G01N21/783 , G01N21/80 , G01N33/004 , G01N2021/773 , Y02A50/244 , Y10T436/204998
摘要： The present invention relates to a device for determining carbon dioxide in a gaseous or liquid sample, comprising: a polymer matrix and an indicator embedded in the polymer matrix, wherein the indicator comprises a pH-sensitive dye and a lipophilic, metal cation-ionophore complex, and wherein an anion of the pH-sensitive dye and the metal cation-ionophore complex form an ion-pair which is soluble in the polymer matrix.
摘要翻译： 本发明涉及一种用于测定气态或液态样品中二氧化碳的装置，包括：聚合物基体和嵌入聚合物基质中的指示剂，其中指示剂包括pH敏感染料和亲脂性金属阳离子离子载体复合物 并且其中所述pH敏感染料和所述金属阳离子离子载体络合物的阴离子形成可溶于所述聚合物基质的离子对。
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6.发明授权公开(公告)号：US06303386B2
公开(公告)日：2001-10-16
申请号：US09091907
申请日：1998-06-24
申请人： Ingo Klimant , Gerhard Holst
发明人： Ingo Klimant , Gerhard Holst
IPC分类号： G01N2164
CPC分类号： G01N21/7703 , G01K11/3213 , G01N21/6428 , G01N2021/772 , G01N2021/7786
摘要： The invention relates to a device for the temperature-compensated determination of chemical parameters, comprising: an optical temperature sensor which contains a temperature indicator that has a temperature-dependent afterglow time and/or luminous intensity and does not react with the surrounding medium; a chemical sensor which contains an indicator that is sensitive to a chemical parameter; means for stimulating the temperature indicator and the chemical indicator to luminesce; means for measuring the luminescence of the temperature indicator and of the chemical indicator; means for establishing an optical connection between indicator, excitation device and measuring equipment; means for detecting luminous radiation.
摘要翻译： 本发明涉及一种用于温度补偿确定化学参数的装置，包括：光学温度传感器，其包含具有温度依赖性余辉时间和/或发光强度并且不与周围介质反应的温度指示器; 包含对化学参数敏感的指示剂的化学传感器; 用于刺激温度指示器和化学指示剂发光的手段; 用于测量温度指示器和化学指示器的发光的装置; 用于在指示器，励磁装置和测量设备之间建立光学连接的装置; 用于检测发光辐射的装置。
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公开(公告)号：US10508992B2
公开(公告)日：2019-12-17
申请号：US13879933
申请日：2010-10-22
摘要： The invention is based on the use of photoluminescent probes for intracellular sensing of oxygen, especially assaying intracellular oxygen concentration. The photoluminescent probe comprises a suspension of polymeric particles having an average diameter in the 20 nm to 100 nm range, formed from an amphiphilic cationic co-polymer which is oriented in the formed particle to provide a hydrophobic core and a hydrophilic shell. The probe includes a hydrophobic oxygen-sensitive photoluminescent dye such as Pt-tetrakis(pentafluorophenyl)porphine, PtPFPP embedded in the hydrophobic core of the particle, and the co-polymer includes quaternary ammonium groups which provide hydrophilic and cationic character to the particle shell. The photoluminescent probe, which in use is provided in the form of an aqueous suspension of probe, is incubated with live mammalian cells in a suitable growth medium for a period of time to allow the probe particles passively load into the cells. Oxygen can then be sensed by detecting a photoluminescent signal of the photoluminescent probe, which can be correlated with oxygen status, for example oxygen concentration or changes in oxygen concentration/levels, using existing techniques.
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公开(公告)号：US07390673B2
公开(公告)日：2008-06-24
申请号：US10476639
申请日：2002-04-08
申请人： Ingo Klimant , Achim Stangelmayer
发明人： Ingo Klimant , Achim Stangelmayer
CPC分类号： G01N33/1846 , G01N21/783 , G01N31/223 , G01N33/182 , Y10T436/25375 , Y10T436/25875
摘要： The invention relates to a method for fluorimetrically or photometrically identifying gaseous substances or substances, which can be converted into a gaseous state, in samples. Decomposition reactions, optional purification steps, and detection can be carried out in a cell, which comprises one or more ion-permeable membranes, in a user-friendly and selective manner without losing any substances.
摘要翻译： 本发明涉及一种用于在样品中荧光测定或光度测定可被转化为气态的气态物质或物质的方法。 分解反应，任选的纯化步骤和检测可以在不损失任何物质的情况下，以用户友好和选择性方式在包含一种或多种离子可渗透膜的细胞中进行。
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公开(公告)号：US06770220B1
公开(公告)日：2004-08-03
申请号：US10031506
申请日：2002-01-15
申请人： Ingo Klimant
发明人： Ingo Klimant
IPC分类号： G01N3358
CPC分类号： B82Y30/00 , B82Y15/00 , G01N33/533 , G01N33/582 , Y10S530/811 , Y10S530/816
摘要： Luminescent microparticles and nanoparticles are provided for use either as internal standards for referencing fluorescent signals, or as markers for detecting biomolecules. Luminescence dyes are incorporated in inert form into solid materials such that they are protected from the influence of chemical and biological compounds in aqueous sample constituents. The photophysical characteristics of the dyes (spectral characteristics, luminescence quantum efficiency, luminescence fading time and polarization) remain unaffected. The surface of the nanoparticles and microparticles can be provided with reactive surfaces in order to enable covalent coupling of biochemicals or to eliminate aggregation.
摘要翻译： 提供发光微粒和纳米颗粒用作参考荧光信号的内部标准，或用作检测生物分子的标记物。 发光染料以惰性形式并入固体材料中，使得它们免受化学和生物化合物在含水样品组分中的影响。 染料的光物理特性（光谱特性，发光量子效率，发光衰减时间和极化）不受影响。 纳米颗粒和微粒的表面可以提供反应性表面，以使生物化学品共价偶联或消除聚集。
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公开(公告)号：US09950111B2
公开(公告)日：2018-04-24
申请号：US13377500
申请日：2010-06-02
申请人： Hans Köhler , Ingo Klimant
发明人： Hans Köhler , Ingo Klimant
IPC分类号： A61M5/172 , A61B5/145 , A61B5/1455 , A61B5/1459 , A61B5/00
CPC分类号： A61M5/1723 , A61B5/14532 , A61B5/14539 , A61B5/14542 , A61B5/14556 , A61B5/1459 , A61B5/415 , A61M2005/1726 , A61M2205/3306 , A61M2230/201 , A61M2230/205 , A61M2230/208
摘要： A device for the transcutaneous, in vivo measurement of the concentration of at least one analyte in a living organism includes a catheter which can be introduced into the organism, and a luminescence indicator, which is immobilized on the catheter and which reacts to a change in the concentration of the at least one analyte to be measured with a change in at least one optical property. The luminescence indicator is transcutaneously connected to a source for providing an excitation radiation and a detector for detecting the measuring radiation. The luminescence indicator is immobilized on the outer circumference of the catheter, which is used to dispense a fluid medium, for example a medication, into the organism or to drain a body fluid.
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