公开(公告)号：US20220002755A1

公开(公告)日：2022-01-06

申请号：US17363942

申请日：2021-06-30

Applicant: Inscripta, Inc.

Inventor： Stephen Tanner ,  Michael Graige ,  Craig Struble ,  Nandini Krishnamurthy ,  Andrea Halweg-Edwards ,  Bryan Leland ,  Simon Cawley ,  Joshua Shorenstein ,  Timothy Shaver

IPC: C12N15/90 ,  C12N15/11 ,  C12N9/22

Abstract: The present disclosure relates to compositions, methods, modules and automated integrated instrumentation to make auxiliary or ancillary edits—in addition to intended edits and immunizing edits—in cellular genomic nucleic acids in order to assess and improve nucleic acid-guided nuclease or nickase fusion editing.

公开(公告)号：US20220002725A1

公开(公告)日：2022-01-06

申请号：US17363884

申请日：2021-06-30

Applicant: Inscripta, Inc.

Inventor： Stephen Tanner ,  Michael Graige ,  Nandini Krishnamurthy ,  Craig Struble

IPC: C12N15/113 ,  C12N9/22 ,  C12N15/85

Abstract: The present disclosure relates to compositions, methods, modules and automated, integrated instrumentation to enable nucleic acid-guided nuclease or nickase fusion editing in cells and correlating the edits to the resulting cellular nucleic acid profile. In some embodiments, methylated bases in a repair template are substituted for unmethylated bases in the cellular target genome and in some embodiments, unmethylated bases are substituted for methylated bases in the cellular target genome.