公开(公告)号：US20220267812A1

公开(公告)日：2022-08-25

申请号：US17676720

申请日：2022-02-21

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Daniel Held ,  Eric Abbate ,  Katherine Noon

IPC: C12P7/10 ,  C12N15/10 ,  C12N1/20 ,  C12N15/70 ,  C12N9/22 ,  C12N15/11 ,  C12N15/52 ,  C10L1/02

Abstract: The present disclosure provides multiplexed engineered cells, automated multi-module instruments and methods of producing biofuel producing cells for enhanced production of biofuels. This platform empowers users to design genetic variant libraries of insertions, swaps, and/or deletions, that can be intentionally or randomly positioned across the entire genome to generate engineered cell populations with improved properties for several common biofuel applications including, but not limited to, improved tolerance to biomass inhibitors, increased thermo-tolerance, increased ethanol production and/or tolerance, expanded carbon utilization abilities, and increased utilization of heterologous proteins or pathways.