公开(公告)号：US20220033829A1

公开(公告)日：2022-02-03

申请号：US17387780

申请日：2021-07-28

Applicant: Inscripta, Inc.

Inventor： Tyson Shepherd ,  Andrew Garst ,  Daniel Held ,  Eric Abbate

IPC: C12N15/63 ,  C12N9/22 ,  C12N15/11

Abstract: The present disclosure relates to compositions, methods, modules and automated integrated instrumentation for using sets of short, curated peptide barcodes to track nucleic acid-guided edits and the translated proteins that result from the edits as well as to create in vitro pathways.

公开(公告)号：US11236441B2

公开(公告)日：2022-02-01

申请号：US17377272

申请日：2021-07-15

Applicant: Inscripta, Inc.

Inventor： Richard Fox ,  Daniel Held

IPC: C12N9/22 ,  C40B50/06 ,  C40B30/04 ,  C40B40/10 ,  C07K16/28 ,  C07K14/74 ,  C40B70/00 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C40B20/04 ,  C40B40/02 ,  C12N15/10

Abstract: The present disclosure provides shuttle vectors for editing exogenous polynucleotides in heterologous live cells, as well as automated methods, modules, and multi-module cell editing instruments and systems for performing the editing methods.

公开(公告)号：US20220002907A1

公开(公告)日：2022-01-06

申请号：US17377272

申请日：2021-07-15

Applicant: Inscripta, Inc.

Inventor： Richard Fox ,  Daniel Held

IPC: C40B50/06 ,  C12N9/22 ,  C40B30/04 ,  C40B40/10 ,  C07K16/28 ,  C07K14/74 ,  C40B70/00 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C40B20/04 ,  C40B40/02 ,  C12N15/10

Abstract: The present disclosure provides shuttle vectors for editing exogenous polynucleotides in heterologous live cells, as well as automated methods, modules, and multi-module cell editing instruments and systems for performing the editing methods.

公开(公告)号：US11512297B2

公开(公告)日：2022-11-29

申请号：US17522399

申请日：2021-11-09

Applicant: Inscripta, Inc.

Inventor： Andrew Garst ,  Tian Tian ,  Daniel Held

IPC: C12N9/22 ,  C12N15/11 ,  C12N15/90

Abstract: The present disclosure provides compositions and methods to increase the percentage of edited cells in a cell population when employing nucleic-acid guided editing, as well as automated multi-module instruments for performing these methods. Specifically, the disclosure relates to methods, compositions, modules and automated multi-module cell processing instruments that increase the efficiency of nucleic acid-guided editing in a cell population using a nucleic acid nuclease (i.e., an RNA-guided nuclease or “RGN”)/single-strand binding protein (“SSB”) fusion system. The system leverages a single-strand binding protein (SSP) and single-strand DNA annealing protein (“SSAP”) interactions to drive enhanced recruitment.

公开(公告)号：US20220333273A1

公开(公告)日：2022-10-20

申请号：US17728735

申请日：2022-04-25

Applicant: Inscripta, Inc.

Inventor： Richard Fox ,  Daniel Held

IPC: C40B50/06 ,  C12N9/22 ,  C40B30/04 ,  C40B40/10 ,  C07K16/28 ,  C07K14/74 ,  C40B70/00 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C40B20/04 ,  C40B40/02 ,  C12N15/10

Abstract: The present disclosure provides shuttle vectors for editing exogenous polynucleotides in heterologous live cells, as well as automated methods, modules, and multi-module cell editing instruments and systems for performing the editing methods.

公开(公告)号：US11085131B1

公开(公告)日：2021-08-10

申请号：US17232046

申请日：2021-04-15

Applicant: Inscripta, Inc.

Inventor： Richard Fox ,  Daniel Held

IPC: C12N9/22 ,  C40B50/06 ,  C40B30/04 ,  C40B40/10 ,  C07K16/28 ,  C07K14/74 ,  C40B70/00 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C40B20/04 ,  C40B40/02 ,  C12N15/10

Abstract: The present disclosure provides shuttle vectors for editing exogenous polynucleotides in heterologous live cells, as well as automated methods, modules, and multi-module cell editing instruments and systems for performing the editing methods.

公开(公告)号：US11078458B2

公开(公告)日：2021-08-03

申请号：US17159137

申请日：2021-01-26

Applicant: Inscripta, Inc.

Inventor： Richard Fox ,  Daniel Held ,  Eric Abbate ,  Michael Clay ,  Katherine Krouse ,  Nandini Krishnamurthy ,  Krishna Yerramsetty

IPC: C12N1/20 ,  C07K14/245

Abstract: The present disclosure relates to various different types of variants in E. coli coding and noncoding regions leading to enhanced lysine production for, e.g., supplements and nutraceuticals.

公开(公告)号：US10995424B2

公开(公告)日：2021-05-04

申请号：US16950878

申请日：2020-11-17

Applicant: Inscripta, Inc.

Inventor： Richard Fox ,  Daniel Held

IPC: C12N9/22 ,  C40B50/06 ,  C40B30/04 ,  C40B40/10 ,  C07K16/28 ,  C07K14/74 ,  C40B70/00 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C40B20/04 ,  C40B40/02 ,  C12N15/10

Abstract: The present disclosure provides shuttle vectors for editing exogenous polynucleotides in heterologous live cells, as well as automated methods, modules, and multi-module cell editing instruments and systems for performing the editing methods.

公开(公告)号：US20210095394A1

公开(公告)日：2021-04-01

申请号：US16950878

申请日：2020-11-17

Applicant: Inscripta, Inc.

Inventor： Richard Fox ,  Daniel Held

IPC: C40B50/06 ,  C12N9/22 ,  C40B30/04 ,  C40B40/10 ,  C07K16/28 ,  C07K14/74 ,  C40B70/00 ,  C12N15/81 ,  C12N15/85 ,  C12N15/90 ,  C40B20/04 ,  C40B40/02 ,  C12N15/10

Abstract: The present disclosure provides shuttle vectors for editing exogenous polynucleotides in heterologous live cells, as well as automated methods, modules, and multi-module cell editing instruments and systems for performing the editing methods.

公开(公告)号：US11787841B2

公开(公告)日：2023-10-17

申请号：US17319006

申请日：2021-05-12

Applicant: Inscripta, Inc.

Inventor： Eric Abbate ,  Katherine Krouse ,  Richard Fox ,  Daniel Held ,  Michael Clay ,  Nandini Krishnamurthy

IPC: A61K39/108 ,  C07K14/245

CPC classification number: C07K14/245 ,  A61K39/0258

Abstract: The present disclosure relates to various different types of mutations in the thrA gene in E. coli leading to enhanced lysine production for, e.g., supplements and nutraceuticals.