公开(公告)号：US20180274004A1

公开(公告)日：2018-09-27

申请号：US15867555

申请日：2018-01-10

申请人： Institute for Environmental Health, Inc.

发明人： Michael Sandor Koeris ,  Michael Cappillino ,  Edyta Krzymanska-Olejnik ,  Daniel Robert Brownell ,  Robert Patrick Shivers ,  Jayson L. Bowers ,  Timothy Kuan-Ta Lu

IPC分类号： C12Q1/04 ,  C12N7/00

CPC分类号： C12Q1/04 ,  C12N7/00 ,  C12N2795/10321 ,  C12N2795/10322 ,  C12N2795/10331 ,  C12N2800/22

摘要： Composition and methods for the detection of one or more target microbe(s) are provided. Compositions of the disclosure include at least one recombinant phage capable of infecting a target microbe, said phage comprising at least a capsid protein sequence, a ribosome binding site, and a codon-optimized marker. Compositions of the disclosure may further include an aqueous solution that enhances the ability to detect marker expression upon phage infection of the target microbe. In some embodiments the target microbe is Listeria.

公开(公告)号：US10273460B2

公开(公告)日：2019-04-30

申请号：US15087151

申请日：2016-03-31

申请人： Institute for Environmental Health, Inc.

发明人： Michael Sandor Koeris ,  Robert Patrick Shivers ,  Daniel Robert Brownell ,  Jason Wyatt Holder ,  Jayson Linn Bowers

IPC分类号： C12N7/00 ,  C12Q1/04

摘要： Methods of detecting target bacteria are provided. In some embodiments, the methods comprise exposing a sample to a phage capable of infecting target bacteria, said phage comprising a heterologous nucleic acid sequence encoding a marker. In some embodiments, the target bacteria comprise Listeria. In some embodiments, the target bacteria are all Listeria. Recombinant Listeria phage comprising a heterologous nucleic acid sequence encoding a marker are also provided as are useful combinations of such phage and articles of manufacture comprising such phage, among other things.

公开(公告)号：US10039795B2

公开(公告)日：2018-08-07

申请号：US14707847

申请日：2015-05-08

申请人： Institute for Environmental Health, Inc.

发明人： Michael Sandor Koeris ,  Jayson L. Bowers ,  Daniel Robert Brownell ,  Edyta Krzymanska-Olejnik ,  Robert Patrick Shivers ,  Michael Cappillino

IPC分类号： C12Q1/70 ,  A61K35/76 ,  C12Q1/66 ,  C12Q1/04 ,  C12N7/00

摘要： Composition and methods for the detection of one or more target microbe(s) are provided. Compositions of the disclosure include at least one recombinant phage capable of infecting a target microbe, said phage comprising at least a capsid protein sequence, a ribosome binding site, and a codon-optimized marker. Compositions of the disclosure may further include an aqueous solution that enhances the ability to detect marker expression upon phage infection of the target microbe. In some embodiments the target microbe include is Listeria.