公开(公告)号：US20150136604A1

公开(公告)日：2015-05-21

申请号：US14500846

申请日：2014-09-29

Applicant: IntegenX Inc.

Inventor： William D. NIELSEN ,  Richard J. BELCINSKI ,  Gregory BOGDAN ,  David EBERHART ,  Omar EL-SISSI ,  Stevan B. JOVANOVICH ,  Michael RECKNOR ,  Ezra VAN GELDER ,  David W. WYRICK

IPC: G01N27/447 ,  G01V3/00 ,  C12Q1/68 ,  G01V8/10 ,  B01L3/00 ,  B01L9/00

CPC classification number: B01L3/502715 ,  B01F13/0059 ,  B01F13/0818 ,  B01L3/5029 ,  B01L3/527 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2200/143 ,  B01L2300/023 ,  B01L2300/027 ,  B01L2300/0672 ,  B01L2300/123 ,  B01L2400/0421 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/0616 ,  B01L2400/0638 ,  G01N27/44743 ,  G01N27/44791 ,  G01N2035/00158 ,  G01N2035/0091

Abstract: This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.