公开(公告)号：US20240382579A1

公开(公告)日：2024-11-21

申请号：US18683835

申请日：2022-08-19

Applicant: Intervet Inc.

Inventor： Erwin Van Den Born ,  Carina Leifeld ,  Amaya Serrano Garcia ,  Holger Honemann ,  Alexandra Jimenez Melsio ,  Kimberly Pietersz

IPC: A61K39/135 ,  A61K39/00 ,  A61P37/04 ,  C07K14/005 ,  C12N15/86

Abstract: The invention concerns a method of producing a foot and mouth disease virus (FMDV) virus-like particle (VLP) in a baculovirus expression system, the method comprising the steps of (i) infecting an insect cell with a baculovirus expression vector, (ii) culturing the insect cell in cell culture medium for 4 days or more post infection, (iii) separating the insect cells from the cell culture to obtain cell-free cell culture medium, and (iv) harvesting the FMDV VLP from the cell-free cell culture medium. The invention further relates to a vaccine for use in the protection of a subject against an infection with FMDV, the vaccine being obtainable by the method of the invention.

公开(公告)号：US20240358815A1

公开(公告)日：2024-10-31

申请号：US18684161

申请日：2022-08-19

Applicant: Intervet Inc.

Inventor： Erwin Van Den Born ,  Carina Leifeld ,  Amaya Serrano Garcia ,  Holger Hönemann ,  Alexandra Jimenez Melsio ,  Kimberly Pietersz

IPC: A61K39/135 ,  A61K39/00

CPC classification number: A61K39/135 ,  A61K2039/5258 ,  C12N2770/32123 ,  C12N2770/32134 ,  C12N2770/32152

Abstract: The invention concerns a method of producing a foot and mouth disease virus (FMDV) virus-like particle (VLP) in a baculovirus expression system, the method comprising the steps of (i) infecting an insect cell with a baculovirus expression vector, (ii) culturing the insect cell in cell culture medium for 5 days or more post infection and (iii) harvesting the FMDV VLP from the cell culture medium. The invention further relates to a vaccine for use in the protection of a subject against an infection with FMDV, the vaccine being obtainable by the method of the invention.