公开(公告)号：US09121010B1

公开(公告)日：2015-09-01

申请号：US13662104

申请日：2012-10-26

申请人： The United States of America, as Represented by the Secretary of Agriculture

发明人： Michael A Larocco ,  Barry Baxt ,  Hernando Duque

IPC分类号： C12N15/85 ,  G01N33/53 ,  C12N5/071

CPC分类号： C12N5/0684 ,  C07K14/70546 ,  C12N2510/00 ,  C12N2710/24211 ,  C12N2710/24251 ,  C12N2760/20011 ,  C12N2760/20051 ,  C12N2770/16011 ,  C12N2770/16051 ,  C12N2770/32111 ,  C12N2770/32151 ,  C12N2770/32152 ,  C12N2770/32311 ,  C12N2770/32351 ,  C12Q1/04 ,  G01N33/56983

摘要： Foot and out disease virus (FMDV) is worldwide problem. Rapid isolation, serotyping and vaccine matching of FMDV from infected animals is critical to enable the implementation of effective vaccination programs and to stop the spread of infection during outbreaks. Current virus isolation protocols use primary cells, known to be susceptible to FMDV, or baby hamster kidney cells (BHK-21) and other cell lines that are not highly sensitive to some strains of FMDV. The αVβ6 integrin is a principal receptor for FMDV. We therefore transduced the porcine kidney cell line, LFBK, to stably express both the αV and β6 bovine integrin subunits. The LFBK-αVβ6 cell line showed both β6 expression and enhanced susceptibility to FMDV infection for at least 100 cell passages. LFBK-αVβ6 cells are highly sensitive for detecting all serotypes of FMDV from experimentally infected animals, including the porcinophillic strain O/TAW/97 and are thus a sensitive tool for FMDV isolation.

摘要翻译： 足病病毒（FMDV）是全世界的问题。 FMDV与受感染动物的快速隔离，血清分型和疫苗匹配对于实施有效的疫苗接种程序并阻止疫情在疫情蔓延中至关重要。 目前的病毒分离方案使用已知对FMDV敏感的原代细胞或对仓鼠肾细胞（BHK-21）和对一些FMDV菌株不敏感的其他细胞系。 αV＆bgr; 6整合素是FMDV的主要受体。 因此，我们转导猪肾细胞系LFBK，以稳定表达αV和bgr。6牛整联蛋白亚基。 LFBK-αV＆bgr。6细胞系显示出至少100个细胞传代的第6个表达和增强的FMDV感染易感性。 LFBK-αV＆bgr; 6细胞对于检测来自实验感染的动物的所有FMDV血清型是非常敏感的，包括噬菌体毒株O / TAW / 97，因此是FMDV分离的敏感工具。

公开(公告)号：US20240358816A1

公开(公告)日：2024-10-31

申请号：US18684529

申请日：2022-06-29

申请人： Intervet Inc. ,  The Pirbright Institute

发明人： Erwin Van Den Born ,  Ian Jones

IPC分类号： A61K39/135 ,  A61K39/00 ,  A61P37/04 ,  C07K14/005 ,  C12N15/86

CPC分类号： A61K39/135 ,  A61P37/04 ,  C07K14/005 ,  C12N15/86 ,  A61K2039/5258 ,  C12N2710/14044 ,  C12N2770/32122 ,  C12N2770/32123 ,  C12N2770/32134 ,  C12N2770/32152 ,  C12N2770/32171

摘要： The present invention provides a recombinant foot and mouth disease virus (FMDV) capsid precursor protein comprising a modified VP1 protein and optionally further comprising a modified VP4 protein. The invention further relates to an isolated nucleic acid molecule and an expression vector comprising the nucleic acid molecule for recombinant expression of the modified capsid precursor protein. In further aspects, the invention relates to a virus-like particle (VLP) obtained from the modified capsid precursor protein and a vaccine for use in the protection of a subject against an infection with FMDV produced from the VLP.

公开(公告)号：US20240358815A1

公开(公告)日：2024-10-31

申请号：US18684161

申请日：2022-08-19

申请人： Intervet Inc.

发明人： Erwin Van Den Born ,  Carina Leifeld ,  Amaya Serrano Garcia ,  Holger Hönemann ,  Alexandra Jimenez Melsio ,  Kimberly Pietersz

IPC分类号： A61K39/135 ,  A61K39/00

CPC分类号： A61K39/135 ,  A61K2039/5258 ,  C12N2770/32123 ,  C12N2770/32134 ,  C12N2770/32152

摘要： The invention concerns a method of producing a foot and mouth disease virus (FMDV) virus-like particle (VLP) in a baculovirus expression system, the method comprising the steps of (i) infecting an insect cell with a baculovirus expression vector, (ii) culturing the insect cell in cell culture medium for 5 days or more post infection and (iii) harvesting the FMDV VLP from the cell culture medium. The invention further relates to a vaccine for use in the protection of a subject against an infection with FMDV, the vaccine being obtainable by the method of the invention.

公开(公告)号：US11952401B2

公开(公告)日：2024-04-09

申请号：US17375203

申请日：2021-07-14

申请人： Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences

发明人： Haixue Zheng ,  Fan Yang ,  Zixiang Zhu ,  Xiangle Zhang ,  Weijun Cao ,  Congcong Wang ,  Kangli Li ,  Huisheng Liu ,  Hong Tian ,  Keshan Zhang ,  Xiangtao Liu

IPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/135 ,  A61P31/14 ,  A61P37/06 ,  C12N7/00

CPC分类号： C07K14/005 ,  A61K39/135 ,  A61P31/14 ,  A61P37/06 ,  C12N7/00 ,  A61K2039/525 ,  A61K2039/552 ,  C12N2770/32121 ,  C12N2770/32122 ,  C12N2770/32134 ,  C12N2770/32152 ,  C12N2770/32171

摘要： The present disclosure belongs to the technical field of biological products for veterinary medicine, and specifically relates to a recombinant foot-and-mouth disease virus (FMDV) with a reduced immunosuppressive activity, a preparation method and use thereof, and a recombinant vaccine strain. According to the present disclosure, it is firstly discovered that FMDV 3B protein has an immunosuppressive function, and key sites for exerting the immunosuppressive function are found. A recombinant FMDV vaccine strain with a lost immunosuppressive function in FMDV 3B protein is constructed by introducing amino acid mutations into three repeated copies of FMDV 3B protein.