公开(公告)号：US20240382579A1

公开(公告)日：2024-11-21

申请号：US18683835

申请日：2022-08-19

Applicant: Intervet Inc.

Inventor： Erwin Van Den Born ,  Carina Leifeld ,  Amaya Serrano Garcia ,  Holger Honemann ,  Alexandra Jimenez Melsio ,  Kimberly Pietersz

IPC: A61K39/135 ,  A61K39/00 ,  A61P37/04 ,  C07K14/005 ,  C12N15/86

Abstract: The invention concerns a method of producing a foot and mouth disease virus (FMDV) virus-like particle (VLP) in a baculovirus expression system, the method comprising the steps of (i) infecting an insect cell with a baculovirus expression vector, (ii) culturing the insect cell in cell culture medium for 4 days or more post infection, (iii) separating the insect cells from the cell culture to obtain cell-free cell culture medium, and (iv) harvesting the FMDV VLP from the cell-free cell culture medium. The invention further relates to a vaccine for use in the protection of a subject against an infection with FMDV, the vaccine being obtainable by the method of the invention.

公开(公告)号：US20240358816A1

公开(公告)日：2024-10-31

申请号：US18684529

申请日：2022-06-29

Applicant: Intervet Inc. ,  The Pirbright Institute

Inventor： Erwin Van Den Born ,  Ian Jones

IPC: A61K39/135 ,  A61K39/00 ,  A61P37/04 ,  C07K14/005 ,  C12N15/86

CPC classification number: A61K39/135 ,  A61P37/04 ,  C07K14/005 ,  C12N15/86 ,  A61K2039/5258 ,  C12N2710/14044 ,  C12N2770/32122 ,  C12N2770/32123 ,  C12N2770/32134 ,  C12N2770/32152 ,  C12N2770/32171

Abstract: The present invention provides a recombinant foot and mouth disease virus (FMDV) capsid precursor protein comprising a modified VP1 protein and optionally further comprising a modified VP4 protein. The invention further relates to an isolated nucleic acid molecule and an expression vector comprising the nucleic acid molecule for recombinant expression of the modified capsid precursor protein. In further aspects, the invention relates to a virus-like particle (VLP) obtained from the modified capsid precursor protein and a vaccine for use in the protection of a subject against an infection with FMDV produced from the VLP.

公开(公告)号：US20240358815A1

公开(公告)日：2024-10-31

申请号：US18684161

申请日：2022-08-19

Applicant: Intervet Inc.

Inventor： Erwin Van Den Born ,  Carina Leifeld ,  Amaya Serrano Garcia ,  Holger Hönemann ,  Alexandra Jimenez Melsio ,  Kimberly Pietersz

IPC: A61K39/135 ,  A61K39/00

CPC classification number: A61K39/135 ,  A61K2039/5258 ,  C12N2770/32123 ,  C12N2770/32134 ,  C12N2770/32152

Abstract: The invention concerns a method of producing a foot and mouth disease virus (FMDV) virus-like particle (VLP) in a baculovirus expression system, the method comprising the steps of (i) infecting an insect cell with a baculovirus expression vector, (ii) culturing the insect cell in cell culture medium for 5 days or more post infection and (iii) harvesting the FMDV VLP from the cell culture medium. The invention further relates to a vaccine for use in the protection of a subject against an infection with FMDV, the vaccine being obtainable by the method of the invention.

公开(公告)号：US20240053340A1

公开(公告)日：2024-02-15

申请号：US18257896

申请日：2021-12-23

Applicant: INTERVET INC.

Inventor： Erwin Van Den Born ,  Urs Peter Bruderer

IPC: G01N33/569

CPC classification number: G01N33/56983 ,  G01N2333/01 ,  G01N2469/20

Abstract: The invention relates to a diagnostic use of an African Swine Fever Vims CD2v protein, a method, a device, and a kit for the detection of the presence of ASFV antibodies in a test sample, in particular the use thereof in a DIVA immunoassay.