Autofocusing apparatus and method for high resolution microscope system
    1.
    发明授权
    Autofocusing apparatus and method for high resolution microscope system 失效
    高分辨率显微镜系统的自动对焦装置和方法

    公开(公告)号:US06172349B2

    公开(公告)日:2001-01-09

    申请号:US08835216

    申请日:1997-03-31

    IPC分类号: G01B900

    CPC分类号: G01B9/04 G02B21/244

    摘要: A method and apparatus for automatically focusing a high resolution microscope, wherein during setup the operator designates areas within each field of view where a measurement will be taken, and for each area of interest translates the microscope along its optical axis (Z-axis) while measuring the image intensities at discrete subareas within the area of interest. These image intensities are then evaluated, and those having the greatest signal-to-noise ratio and occurring at a common point along the Z-axis will be selected, and the corresponding subareas identified. During subsequent inspections of the area of interest, only light reflected from the identified subareas will be used to focus the microscope. The invention has application in both conventional microscopy and interferometry.

    摘要翻译: 一种用于自动对焦高分辨率显微镜的方法和装置,其中在设置期间,操作者指定将在每个视野内进行测量的区域,并且对于每个感兴趣的区域,显微镜沿其光轴(Z轴)转换,同时 测量感兴趣区域内离散子区域的图像强度。 然后评估这些图像强度,并且将选择具有最大信噪比并且沿着Z轴在公共点处发生的图像强度,并且识别相应的子区域。 在随后对感兴趣的区域进行检查时,只有从识别的子区域反射的光线才能用于对显微镜进行对焦。 本发明可应用于常规显微镜和干涉测量。