公开(公告)号：US20020116988A1

公开(公告)日：2002-08-29

申请号：US09794772

申请日：2001-02-27

申请人： Isco, Inc.

发明人： Dale A Davison ,  Scott L. Blakley

IPC分类号： G01N030/00

CPC分类号： G01N35/1097 ,  B01D15/12 ,  B01D15/166 ,  B01D15/247 ,  G01N1/38 ,  G01N30/32 ,  G01N30/466 ,  G01N2001/383 ,  G01N2030/324 ,  G01N2030/326

摘要： To economically perform preparatory chromatography, a plurality of pumps each having a corresponding one of a plurality of pistons and a corresponding one of a plurality of cylinders are driven by one motor to draw and pump solvent simultaneously into corresponding columns. To form a gradient the pumps are connected to two-way valves that are connected alternately to a first solvent and a second solvent, whereby the time said valve is in a first position controls the amount of solvent drawn from said first reservoir into said pumps and the amount of time in said second position controls the amount of said second solvent drawn into said pumps and the solvent is mixed in the pumping systems. The detectors are photodiodes mounted to light guides in the flow cells that generate signals related to light absorbance and communicate with a controller, whereby said controller receives signals indicating solute between the light guides and causes collection of solute.

摘要翻译： 为了经济地进行准备色谱，每个具有多个活塞中的相应一个活塞和多个气缸中的相应一个的多个泵由一个电动机驱动，以将溶剂同时泵送到相应的列中。 为了形成梯度，泵连接到交替地连接到第一溶剂和第二溶剂的双向阀，由此所述阀处于第一位置的时间控制从所述第一储存器抽入所述泵中的溶剂的量， 所述第二位置中的时间量控制吸入所述泵中的所述第二溶剂的量并且所述溶剂在所述泵送系统中混合。 检测器是安装在流动池中的光导的光电二极管，其产生与吸光度相关的信号并与控制器通信，由此所述控制器接收指示光导之间的溶质并引起溶质的收集的信号。