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公开(公告)号：US07211790B2
公开(公告)日：2007-05-01
申请号：US11132174
申请日：2005-05-19
CPC分类号： G01N30/8675 , G01N30/8679 , H01J49/0036
摘要： The invention intends to determine a presence ratio between mixed compounds which are difficult to separate from each other by a separation unit and cannot be discriminated by MS. In a mass spectrometer with an MSn analysis function for ionizing a sample eluted from a separation unit for separating the sample into individual components, fragmenting an ion of a desired mass number, and performing a mass analysis of fragment ions, the mass spectrometer includes a database storing correlation information between an isomer presence ratio and a particular ion intensity ratio in a mass spectrum per isomer. An isomer ratio can be clarified even for a mixed sample of isomers, such as enantiomers, which are difficult to separate from each other by the separation unit and cannot be discriminated by MS.
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公开(公告)号：US20050159902A1
公开(公告)日：2005-07-21
申请号：US11037043
申请日：2005-01-19
申请人： Izumi Ogata , Shinya Ito , Yasuhiro Takegawa , Kisaburo Deguchi , Hiroaki Nakagawa , Shin-Ichiro Nishimura
发明人： Izumi Ogata , Shinya Ito , Yasuhiro Takegawa , Kisaburo Deguchi , Hiroaki Nakagawa , Shin-Ichiro Nishimura
CPC分类号： H01J49/0036 , G16C20/20
摘要： In a database MSn spectrum search, search of a known compound whose principal chain is identical to that of an unknown compound is enabled, thereby allowing analysis of an entire structure even if the entire structures of the known compound in database and that of the measured unknown compound are not identical. The MSn spectrum obtained in the MSn measurement of the unknown compound is compared with all the MSm spectra (m≧1) in the database regardless of MSn generation. In the MSn measurement of a series of related compounds including various types of different side chains of the same principal chain, such as in the case of biopolymers, it becomes possible to determine the structure of the principal chain using a database search even if the entire structure is not clear. And the estimation of the entire structure is made possible on the basis of the principal chain structure.
摘要翻译： 在数据库MS搜索中，搜索其主链与未知化合物的主链相同的已知化合物，从而允许分析整个结构，即使已知化合物的整个结构 在数据库中和测量的未知化合物的数据不相同。 将未知化合物的MS 测量中获得的MS 光谱与所有MS谱（m> = 1）进行比较 数据库，无论MS n 生成。 在一系列相关化合物（包括同一主链的各种不同侧链的相关化合物）的测定中，例如在生物聚合物的情况下，可以确定主链的结构 使用数据库搜索即使整个结构不清楚。 而整个结构的估计是可以在主链结构的基础上进行的。
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公开(公告)号：US20050258355A1
公开(公告)日：2005-11-24
申请号：US11132174
申请日：2005-05-19
CPC分类号： G01N30/8675 , G01N30/8679 , H01J49/0036
摘要： The invention intends to determine a presence ratio between mixed compounds which are difficult to separate from each other by a separation unit and cannot be discriminated by MS. In a mass spectrometer with an MSn analysis function for ionizing a sample eluted from a separation unit for separating the sample into individual components, fragmenting an ion of a desired mass number, and performing a mass analysis of fragment ions, the mass spectrometer includes a database storing correlation information between an isomer presence ratio and a particular ion intensity ratio in a mass spectrum per isomer. An isomer ratio can be clarified even for a mixed sample of isomers, such as enantiomers, which are difficult to separate from each other by the separation unit and cannot be discriminated by MS.
摘要翻译： 本发明意在确定难以通过分离单元彼此分离并且不能被MS鉴别的混合化合物之间的存在比例。 在具有MS分析功能的质谱仪中，用于将从分离单元洗脱的样品离子化以将样品分离成单个组分，使所需质量数的离子分段，并进行片段的质量分析 离子，质谱仪包括存储每个异构体的质谱中异构体存在比和特定离子强度比之间相关信息的数据库。 甚至对于异构体的混合样品（如对映异构体）也可以澄清异构体比例，这些异构体难以通过分离单元彼此分离并且不能被MS鉴别。
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公开(公告)号：US07462817B2
公开(公告)日：2008-12-09
申请号：US11723995
申请日：2007-03-23
CPC分类号： G01N30/8675 , G01N30/8679 , H01J49/0036
摘要： The invention intends to determine a presence ratio between mixed compounds which are difficult to separate from each other by a separation unit and cannot be discriminated by MS. In a mass spectrometer with an MSn analysis function for ionizing a sample eluted from a separation unit for separating the sample into individual components, fragmenting an ion of a desired mass number, and performing a mass analysis of fragment ions, the mass spectrometer includes a database storing correlation information between an isomer presence ratio and a particular ion intensity ratio in a mass spectrum per isomer. An isomer ratio can be clarified even for a mixed sample of isomers, such as enantiomers, which are difficult to separate from each other by the separation unit and cannot be discriminated by MS.
摘要翻译： 本发明意在确定难以通过分离单元彼此分离并且不能被MS鉴别的混合化合物之间的存在比例。 在具有MSn分析功能的质谱仪中，用于将从分离单元洗脱的样品离子化，用于将样品分离成单独的组分，将所需质量数的离子分段，并进行碎片离子的质量分析，质谱仪包括数据库 在每个异构体的质谱中存储异构体存在比和特定离子强度比之间的相关信息。 甚至对于异构体的混合样品（如对映异构体）也可以澄清异构体比例，这些异构体难以通过分离单元彼此分离并且不能被MS鉴别。
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公开(公告)号：US20070164212A1
公开(公告)日：2007-07-19
申请号：US11723995
申请日：2007-03-23
IPC分类号： H01J49/00
CPC分类号： G01N30/8675 , G01N30/8679 , H01J49/0036
摘要： The invention intends to determine a presence ratio between mixed compounds which are difficult to separate from each other by a separation unit and cannot be discriminated by MS. In a mass spectrometer with an MSn analysis function for ionizing a sample eluted from a separation unit for separating the sample into individual components, fragmenting an ion of a desired mass number, and performing a mass analysis of fragment ions, the mass spectrometer includes a database storing correlation information between an isomer presence ratio and a particular ion intensity ratio in a mass spectrum per isomer. An isomer ratio can be clarified even for a mixed sample of isomers, such as enantiomers, which are difficult to separate from each other by the separation unit and cannot be discriminated by MS.
摘要翻译： 本发明意在确定难以通过分离单元彼此分离并且不能被MS鉴别的混合化合物之间的存在比例。 在具有MS分析功能的质谱仪中，用于将从分离单元洗脱的样品离子化以将样品分离成单个组分，使所需质量数的离子分段，并进行片段的质量分析 离子，质谱仪包括存储每个异构体的质谱中异构体存在比和特定离子强度比之间相关信息的数据库。 甚至对于异构体的混合样品（如对映异构体）也可以澄清异构体比例，这些异构体难以通过分离单元彼此分离并且不能被MS鉴别。
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公开(公告)号：US20120021448A1
公开(公告)日：2012-01-26
申请号：US13130293
申请日：2009-11-20
IPC分类号： C12Q1/02
CPC分类号： G01N33/5023 , G01N2400/00
摘要： Provided are a novel marker of the state of cells and the differentiation level of the same which is usable as a substitute for a gene or a protein; a means for quantitatively understanding this marker; and a means for understanding the state of specific cells and the differentiation level of the same using this marker. A method for determining a sugar chain category to be used for evaluating the state of cells. The quantitative profile of N-linked sugar chains of cells before a change in the state thereof is obtained. Next, the quantitative profile of N-linked sugar chains contained in the cells after the change in the state thereof is obtained. Sugar chains showing variations in the contents thereof between these two quantitative profiles are extracted and the individual sugar chains contained in the extracted sugar chains are classified into the following categories based on sugar chain type: either the high-mannose type or the non-high-mannose type; (1) the presence/absence of a bisect; (2) the number fucose residues (0, 1, or 2 or more); (3) the presence/absence of sialic acid; and (4) the degree of branching (2 or 3 or more). From these five categories, a sugar chain category appropriate for evaluating the state of cells is determined. A method for evaluating the state of cells and the differentiation of cells using the thus determined sugar chain category.
摘要翻译： 提供了可用作基因或蛋白质的替代物的细胞状态和分化水平的新型标记物; 一种用于定量了解该标记的方法; 以及使用该标记物来理解特定细胞的状态和其分化水平的方法。 用于测定用于评估细胞状态的糖链类别的方法。 获得其状态改变之前的细胞的N-连接糖链的定量分布。 接下来，获得其状态改变后的细胞中含有的N-连接糖链的定量分布。 提取显示这两种定量分布之间含量变化的糖链，并且基于糖链类型将提取的糖链中包含的各糖链分为以下几类：高甘露糖型或非高糖型， 甘露糖型; （1）二等分的存在/不存在; （2）数字岩藻糖残基（0,1或2以上）; （3）唾液酸的存在/不存在; 和（4）支化度（2或3以上）。 从这五个类别中，确定适合于评估细胞状态的糖链类别。 使用如此确定的糖链类别评估细胞状态和细胞分化的方法。
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7.发明申请CELL SHEET HAVING HYALURONIC ACID PRODUCTION ABILITY AND METHOD FOR PREPARING THE SAME 审中-公开具有羟丙酸生产能力的细胞片及其制备方法公开(公告)号：US20140056858A1
公开(公告)日：2014-02-27
申请号：US14001552
申请日：2012-02-28
摘要： A cell sheet having a high hyaluronic acid production ability can be typically prepared by a method in which, on a cell culture support having a surface coated with a polymer which changes a hydration ability in a temperature range of 0-80° C., cells with increased stem cell content are cultured in a temperature range where the polymer exhibits a weak hydration ability, and then the temperature of a culture solution is changed to a temperature at which the polymer exhibits a strong hydration ability, so that the cultured cells are detached in the form of sheet.
摘要翻译： 通常可以通过以下方法制备具有高透明质酸生产能力的细胞片，其中在具有在0-80℃的温度范围内改变水合能力的聚合物表面涂覆的细胞培养支持物的细胞，细胞 在聚合物表现出弱水合能力的温度范围内培养增加的干细胞含量，然后将培养液的温度改变为聚合物表现出强的水合能力的温度，使得培养的细胞分离 以表格的形式。
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公开(公告)号：US20070166783A1
公开(公告)日：2007-07-19
申请号：US10584065
申请日：2004-12-24
摘要： It is intended to chemically synthesize the trisaccharide moiety at the reducing end in the core sugar chain structure of an asparagine-linked glycoprotein. By using a highly inexpensive natural polysaccharide having a mannoseβ-1,4-bond as the starting material, a β-1,4-glycoside bond of mannose is formed.
摘要翻译： 旨在在天冬酰胺连接的糖蛋白的核心糖链结构的还原端化学合成三糖部分。 通过使用具有甘露糖-b-1,4键的高度廉价的天然多糖作为起始原料，形成甘露糖的β-1,4-糖苷键。
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公开(公告)号：US20130309703A1
公开(公告)日：2013-11-21
申请号：US13988418
申请日：2011-11-18
IPC分类号： G01N21/64
CPC分类号： G01N21/6486 , G01N30/7233 , G01N30/74 , G01N30/96 , G01N2021/6439 , G01N2030/067 , G01N2030/8836
摘要： A method of analyzing a sugar chain by high performance liquid chromatography uses a column packed with a stationary phase including anion and cation exchangers. A fluorescence-labeled sample is prepared for the analysis by decomposing glycosaminoglycans to be analyzed into their constitutional units being disaccharides, capturing the disaccharides by glycoblotting, releasing the disaccharides by acid treatment, and subsequently reductively aminating the released disaccharides. In this method, two or more types of glycosaminoglycans can be simultaneously analyzed.
摘要翻译： 通过高效液相色谱法分析糖链的方法是使用包含阴离子和阳离子交换剂的固定相填充的柱。 制备荧光标记的样品用于通过分解将待分析的糖胺聚糖作为二糖的结构单元进行分析，通过糖印迹捕获二糖，通过酸处理释放二糖，随后还原释放释放的二糖。 在该方法中，可以同时分析两种以上的糖胺聚糖。
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10.发明授权公开(公告)号：US07598372B2
公开(公告)日：2009-10-06
申请号：US10584065
申请日：2004-12-24
摘要： It is intended to chemically synthesize the trisaccharide moiety at the reducing end in the core sugar chain structure of an asparagine-linked glycoprotein. By using a highly inexpensive natural polysaccharide having a mannoseβ-1,4-bond as the starting material, a β-1,4-glycoside bond of mannose is formed.
摘要翻译： 旨在在天冬酰胺连接的糖蛋白的核心糖链结构的还原端化学合成三糖部分。 通过使用具有甘露糖-b-1,4键的高度廉价的天然多糖作为起始原料，形成甘露糖的β-1,4-糖苷键。
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