公开(公告)号：US5700470A

公开(公告)日：1997-12-23

申请号：US615048

申请日：1996-03-12

申请人： Izumu Saito ,  Yumi Kanegae ,  Michio Nakai

发明人： Izumu Saito ,  Yumi Kanegae ,  Michio Nakai

IPC分类号： C12N15/09 ,  C07H21/04 ,  C12N7/00 ,  C12N7/01 ,  C12N15/861 ,  C12R1/92 ,  A61K39/23 ,  A61K39/12 ,  C12N1/20 ,  C12N15/00

CPC分类号： C12N15/86 ,  C12N2710/10343 ,  C12N2800/30

摘要： A recombinant DNA virus for transfecting an animal cell and bearing a foreign gene and a promoter capable of regulating expression of the foreign gene is completely deleted of the function of E2A gene. The recombinant DNA virus can thus stably transduce the foreign gene into various animal cells, which leads to continuous expression of the foreign gene in the animal cells. The continuous expression of the foreign gene can provide an effective treatment of hereditary disease.

摘要翻译： 用于转染动物细胞并携带外源基因的重组DNA病毒和能够调节外源基因表达的启动子完全缺失E2A基因的功能。 因此，重组DNA病毒可以稳定地将外源基因转导到各种动物细胞中，这导致外源基因在动物细胞中的连续表达。 外源基因的连续表达可以提供遗传性疾病的有效治疗。

公开(公告)号：US20060003443A1

公开(公告)日：2006-01-05

申请号：US11217321

申请日：2005-09-02

申请人： Izumu Saito ,  Yumi Kanegae

发明人： Izumu Saito ,  Yumi Kanegae

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12N5/00 ,  C12N5/02

CPC分类号： C12N9/00 ,  C12N2799/022 ,  C12N2800/30

摘要： In accordance with the present invention, there are provided cells for expressing recombinase Cre in the presence of recombinase FLP in a FLP-dependent manner, methods of expressing recombinase Cre by introducing recombinase FLP into the above cells, methods of producing recombinant viral vectors using cells that express recombinase Cre in the presence of recombinase FLP in a FLP-dependent manner, and methods of producing recombinant adenovirus vectors using the above method of producing Cre and the above method of producing recombinant viral vectors.

摘要翻译： 根据本发明，提供了在重组酶FLP存在下以FLP依赖性方式表达重组酶Cre的细胞，通过将重组酶FLP引入上述细胞中表达重组酶Cre的方法，使用细胞产生重组病毒载体的方法 其以FLP依赖性方式在重组酶FLP存在下表达重组酶Cre，以及使用上述制备Cre的方法和上述生产重组病毒载体的方法生产重组腺病毒载体的方法。

公开(公告)号：US20080319177A1

公开(公告)日：2008-12-25

申请号：US12111049

申请日：2008-04-28

申请人： Izumu SAITO ,  Yumi Kanegae

发明人： Izumu SAITO ,  Yumi Kanegae

IPC分类号： C07H21/04

CPC分类号： C12N9/00 ,  C12N2799/022 ,  C12N2800/30

摘要： In accordance with the present invention, there are provided cells for expressing recombinase Cre in the presence of recombinase FLP in a FLP-dependent manner, methods of expressing recombinase Cre by introducing recombinase FLP into the above cells, methods of producing recombinant viral vectors using cells that express recombinase Cre in the presence of recombinase FLP in a FLP-dependent manner, and methods of producing recombinant adenovirus vectors using the above method of producing Cre and the above method of producing recombinant viral vectors.

摘要翻译： 根据本发明，提供了在重组酶FLP存在下以FLP依赖性方式表达重组酶Cre的细胞，通过将重组酶FLP引入上述细胞中表达重组酶Cre的方法，使用细胞产生重组病毒载体的方法 其以FLP依赖性方式在重组酶FLP存在下表达重组酶Cre，以及使用上述制备Cre的方法和上述生产重组病毒载体的方法生产重组腺病毒载体的方法。

公开(公告)号：US5731172A

公开(公告)日：1998-03-24

申请号：US302312

申请日：1994-09-08

申请人： Izumu Saito ,  Yumi Kanegae

发明人： Izumu Saito ,  Yumi Kanegae

IPC分类号： C12N15/861 ,  C12N15/64 ,  C12N15/66

CPC分类号： C12N15/86 ,  C12N2710/10343

摘要： Disclosed are a recombinant adenovirus bearing a DNA sequence encoding a desired foreign polypeptide, and a hybrid promoter (CAG promoter) comprising a cytomegalovirus enhancer, a chicken .beta.-actin promoter, a rabbit .beta.-globin splicing acceptor and a polyA sequence, a process for production thereof, as well as use thereof for in genetic treatment. The recombinant adenovirus effectively expresses the foreign polypeptide in a wide range of animal cells.

摘要翻译： 公开了具有编码期望的外来多肽的DNA序列的重组腺病毒和包含巨细胞病毒增强子，鸡β-肌动蛋白启动子，兔β-珠蛋白剪接受体和polyA序列的杂合启动子（CAG启动子） 其生产，以及其在遗传处理中的用途。 重组腺病毒在广泛的动物细胞中有效地表达外源多肽。

公开(公告)号：US5817492A

公开(公告)日：1998-10-06

申请号：US521575

申请日：1995-08-30

申请人： Izumu Saito ,  Yumi Kanegae

发明人： Izumu Saito ,  Yumi Kanegae

IPC分类号： C12N15/09 ,  A61K48/00 ,  C12N5/10 ,  C12N9/00 ,  C12N15/86 ,  C12N15/861 ,  C12N15/00

CPC分类号： C12N15/86 ,  C12N9/00 ,  A61K48/00 ,  C12N2710/10343 ,  C12N2800/108 ,  C12N2800/30

摘要： An animal cell is co-transfected with both a recombinant DNA viral vector which bears a promoter, a recombinase gene and a poly(A) sequence and a recombinant DNA viral vector which bears two recombinase-recognizing sequences and which further bears an origin of replication, a promoter, a foreign gene and a poly(A) sequence, each of which is located between the two recombinase-recognizing sequences. Thereafter, in the co-transfected animal cell, a DNA fragment containing the origin of replication, promoter, foreign gene and poly(A) sequence is excised from the vector by the action of a recombinase expressed in the another vector. The DNA fragment forms a circular DNA molecule which autonomously replicates in the co-transfected animal cell due to the origin of replication, whereby the foreign gene is continuously expressed. Accordingly, the combination of the above two DNA viral vectors wherein a gene which is defective in patients is used as the foreign gene is quite useful especially for the treatment of the patient with hereditary diseases.

摘要翻译： 用携带启动子，重组酶基因和聚（A）序列的重组DNA病毒载体和携带两个重组酶识别序列并进一步具有复制起点的重组DNA病毒载体共同转染动物细胞 ，启动子，外源基因和poly（A）序列，其各自位于两个重组酶识别序列之间。 此后，在共转染动物细胞中，通过在另一载体中表达的重组酶的作用，从载体中切下含有复制起点，启动子，外源基因和聚（A）序列的DNA片段。 DNA片段形成由于复制起点在共转染的动物细胞中自主复制的环状DNA分子，由此外来基因被连续表达。 因此，上述两种DNA病毒载体的组合，其中使用患者缺陷的基因作为外源基因，特别是用于治疗遗传性疾病的患者是非常有用的。