公开(公告)号：US20170282130A1

公开(公告)日：2017-10-05

申请号：US15599841

申请日：2017-05-19

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Takahiko HARIYAMA ,  Yasuharu TAKAKU ,  Hiroshi SUZUKI ,  Masatsugu SHIMOMURA ,  Daisuke ISHII ,  Isao OHTA ,  Yoshinori MURANAKA

IPC: B01D71/38 ,  B01D67/00 ,  B01D69/12 ,  B01D69/02 ,  C09D4/06 ,  C09D4/00

CPC classification number: B01D71/38 ,  B01D67/009 ,  B01D69/02 ,  B01D69/127 ,  B01D2323/30 ,  B01D2325/36 ,  C09D4/00 ,  C09D4/06

Abstract: Provided is a technique for fabricating a novel thin membrane with a starting material such as a biocompatible compound that is not easily processible into a membrane, particularly a technique for fabricating a novel thin membrane of a composition that is gradually polymerized from a membrane surface into the membrane in the cross sectional direction of the membrane, and having, for example, different structures on the front and back of the membrane. The technique includes the steps of preparing a solution of a starting material compound; forming a thin membrane of the solution on a base material surface; and forming the organic polymer thin membrane through a polymerization reaction caused by irradiating the exposed surface of the thin membrane with a plasma or an electron beam.

公开(公告)号：US20160343539A1

公开(公告)日：2016-11-24

申请号：US15114978

申请日：2015-01-28

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Takahiko HARIYAMA ,  Yasuharu TAKAKU ,  Hiroshi SUZUKI ,  Satoshi HIRAKAWA ,  Hideaki KAWASAKI ,  Masatsugu SHIMOMURA ,  Daisuke ISHII ,  Isao OHTA ,  Yoshinori MURANAKA

IPC: H01J37/20 ,  G01N33/50 ,  H01J37/22 ,  G01N33/483

CPC classification number: H01J37/20 ,  G01N33/4833 ,  G01N33/5011 ,  G01N2223/612 ,  G01N2800/7028 ,  H01J37/22 ,  H01J2237/2002 ,  H01J2237/2007

Abstract: Provided are a protective agent for electron microscopic observation in a vacuum which can protect a biological sample in a water-containing state, such as a mammal, a plant tissue or a cultured cell, and a single cell in the living state without deforming it even in a vacuum, a kit using the same, methods for observation, diagnosis, evaluation, and quantification of a sample by an electron microscope, and a sample stage to be used for the observation. The protective agent for electron microscopic observation of the present invention contains a a component to impart the survival environment, a saccharide, and an electrolyte.

Abstract translation: 提供一种用于真空中电子显微镜观察的保护剂，其可以保护生物状态中的含水状态的生物样品例如哺乳动物，植物组织或培养细胞，以及单一细胞，而不使其变形甚至 在真空中，使用其的试剂盒，通过电子显微镜观察，诊断，评价和定量样品的方法以及用于观察的样品台。 本发明的电子显微镜观察用保护剂含有赋予生存环境的成分，糖类和电解质。