公开(公告)号：US09829477B2

公开(公告)日：2017-11-28

申请号：US14429035

申请日：2013-03-21

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Tsuneo Urisu ,  Zhi-hong Wang ,  Hidetaka Uno ,  Miho Saitoh ,  Yasutaka Nagaoka

IPC: C12M1/32 ,  G01N33/487 ,  C12M1/00 ,  C12M1/26

CPC classification number: G01N33/48728 ,  C12M23/04 ,  C12M23/12 ,  C12M23/38 ,  C12M25/06 ,  C12M33/00 ,  C12M33/04 ,  C12M35/02 ,  C12N5/0619

Abstract: A planar patch clamp device is disclosed, which can be used for culturing a neuron in the device so as to form a neuron network, and detecting an electrical property of the neuron that forms the neuron network. The planar patch clamp device includes a plurality of protrusions formed on a first surface, an extracellular matrix forming substance which is coated on the peripheries of a through hole, and electrode sections.

公开(公告)号：US20150233890A1

公开(公告)日：2015-08-20

申请号：US14429035

申请日：2013-03-21

Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

Inventor： Tsuneo Urisu ,  Zhi-hong Wang ,  Hidetaka Uno ,  Miho Saitoh ,  Yasutaka Nagaoka

IPC: G01N33/487 ,  C12M1/00 ,  C12M1/26 ,  C12M1/32

CPC classification number: G01N33/48728 ,  C12M23/04 ,  C12M23/12 ,  C12M23/38 ,  C12M25/06 ,  C12M33/00 ,  C12M33/04 ,  C12M35/02 ,  C12N5/0619

Abstract: Provided are a culture device for neuronal network formation that makes it possible to construct a neuronal network while restricting movement of neurons grown in the culture medium, and a means for the use thereof. A culture device for neuronal network formation in which is formed a cell fixation part surrounded by a plurality of projections on a flat substrate that can be filled with cell culture medium, and in which (1) there are established spaces having widths such that the neuron cells cannot pass through between the plurality of projections, (2) the inner diameter is such that the cell fixation part can house the cells of from one to a number of neurons, (3) the substrate surface, which is the bottom of the cell fixation part, is coated by an extracellular matrix-forming substance and/or provided with minute through-holes for suctioning culture medium by a suction device on the lower side of the substrate surface.

Abstract translation: 提供了一种用于神经元网络形成的培养装置，其可以构建神经元网络，同时限制在培养基中生长的神经元的运动，以及用于其的手段。 一种用于神经元网络形成的培养装置，其形成有可以填充有细胞培养基的平坦基底上的多个突起包围的细胞固定部分，其中（1）具有宽度使得神经元 细胞不能通过多个突起之间，（2）内径使得细胞固定部分能够容纳从一个到多个神经元的细胞，（3）作为细胞底部的底物表面 固定部分由细胞外基质形成物质涂覆和/或设置有用于通过基底表面下侧的抽吸装置吸引培养基的微小通孔。