公开(公告)号：US20080175468A1

公开(公告)日：2008-07-24

申请号：US11626795

申请日：2007-01-24

申请人： Jack Y. Jau ,  Zhaoli Zhang ,  Wei Fang

发明人： Jack Y. Jau ,  Zhaoli Zhang ,  Wei Fang

IPC分类号： G06T7/00

CPC分类号： G06T7/001 ,  G06K9/6228 ,  G06K9/6253 ,  G06T2207/30148

摘要： A method and system for creating knowledge and selecting features in a supervised classifier is disclosed. The method and system comprises changing a feature space of a plurality of defects and marking at least a portion of the samples of the defects in the feature space. The method and system includes labeling the at least a portion of the samples as training samples, determining if the training samples are of the same type and creating knowledge based upon the training samples if the samples are of the same type.

摘要翻译： 公开了一种在监督分类器中创建知识和选择特征的方法和系统。 该方法和系统包括改变多个缺陷的特征空间并标记特征空间中的缺陷样本的至少一部分。 所述方法和系统包括将所述样本的至少一部分标记为训练样本，确定所述训练样本是否是相同类型，并且如果所述样本是相同类型，则基于所述训练样本创建知识。

公开(公告)号：US08969309B2

公开(公告)日：2015-03-03

申请号：US13811367

申请日：2011-07-20

申请人： Shidong Liu ,  Zhaoli Zhang ,  Zhonghao Zhuo ,  Xiaoming Ji ,  Xiaoliang Gao

发明人： Shidong Liu ,  Zhaoli Zhang ,  Zhonghao Zhuo ,  Xiaoming Ji ,  Xiaoliang Gao

IPC分类号： A61K38/12 ,  C07K7/56 ,  A61K38/00

CPC分类号： C07K7/56 ,  A61K38/00 ,  A61K38/12

摘要： The present invention discloses a crystal of a peptide substance and the preparation method as well as the use thereof. Said crystal B possesses peaks at the following 2θ angles in the X-ray diffraction pattern (XRPD): 3.2±0.2°, 5.4±0.2°, 6.2±0.2°, and 9.3±0.2°.

摘要翻译： 本发明公开了一种肽物质的晶体及其制备方法及其用途。 所述晶体B在以下2°处具有峰值; X射线衍射图（XRPD）中的角度：3.2±0.2°，5.4±0.2°，6.2±0.2°和9.3±0.2°。

公开(公告)号：US08877777B2

公开(公告)日：2014-11-04

申请号：US13877141

申请日：2011-09-27

申请人： Zhaoli Zhang ,  Shidong Liu ,  Zhonghao Zhuo ,  Xiaoming Ji

发明人： Zhaoli Zhang ,  Shidong Liu ,  Zhonghao Zhuo ,  Xiaoming Ji

IPC分类号： A61K38/12 ,  C07K5/00 ,  C07K7/00 ,  C07K16/00 ,  C07K17/00 ,  C07K7/56

CPC分类号： C07K7/56

摘要： A method for purifying a cyclic lipopeptide or a salt thereof is provided. The method comprises the steps: (1) extracting a fermentation broth containing a compound of formula I or a salt thereof, to obtain an extract 1 after filtration or centrifugation; (2) diluting or concentrating the extract 1 under vacuum to decrease the content of the organic solvent, to obtain an extract 2; (3) loading the extract 2 onto a macroporous absorption resin; (4) washing the macroporous adsorption resin with water, an organic solvent, or a mixture of water and an organic solvent as a washing solution; and (5) eluting the compound of formula I off from the macroporous adsorption resin with water, an organic solvent, or a mixture of water and an organic solvent as a washing solution as an eluant. Compared with the prior art, the purification method has the advantages that fewer organic solvent is used, no silica gel is used, the harm to the environment is less, and the purity of the collected compound of formula I is improved.

摘要翻译： 提供了一种纯化环状肽或其盐的方法。 该方法包括以下步骤：（1）提取含有式I化合物或其盐的发酵液，过滤或离心后得到提取物1; （2）真空稀释或浓缩提取物1以降低有机溶剂的含量，得到提取物2; （3）将提取物2装载到大孔吸附树脂上; （4）用水，有机溶剂或水和有机溶剂的混合物洗涤大孔吸附树脂作为洗涤液; 和（5）用水，有机溶剂或水和有机溶剂的混合物作为洗脱液从大孔吸附树脂中除去式I化合物作为洗脱剂。 与现有技术相比，纯化方法的优点是使用少量有机溶剂，不使用硅胶，对环境的危害较小，提取了所收集的式I化合物的纯度。

公开(公告)号：US20130123197A1

公开(公告)日：2013-05-16

申请号：US13811367

申请日：2011-07-20

申请人： Shidong Liu ,  Zhaoli Zhang ,  Zhonghao Zhuo ,  Xiaoming Ji ,  Xiaoliang Gao

发明人： Shidong Liu ,  Zhaoli Zhang ,  Zhonghao Zhuo ,  Xiaoming Ji ,  Xiaoliang Gao

IPC分类号： C07K7/56

CPC分类号： C07K7/56 ,  A61K38/00 ,  A61K38/12

摘要： The present invention discloses a crystal of a peptide substance and the preparation method as well as the use thereof. Said crystal B possesses peaks at the following 2θ angles in the X-ray diffraction pattern (XRPD): 3.2±0.2°, 5.4±0.2°, 6.2±0.2°, and 9.3±0.2°.

摘要翻译： 本发明公开了一种肽物质的晶体及其制备方法及其用途。 所述晶体B在X射线衍射图（XRPD）中具有以下2θ角的峰：3.2±0.2°，5.4±0.2°，6.2±0.2°和9.3±0.2°。

公开(公告)号：US20130296529A1

公开(公告)日：2013-11-07

申请号：US13877141

申请日：2011-09-27

申请人： Zhaoli Zhang ,  Shidong Liu ,  Zhonghao Zhuo ,  Xiaoming Ji

发明人： Zhaoli Zhang ,  Shidong Liu ,  Zhonghao Zhuo ,  Xiaoming Ji

IPC分类号： C07K7/56

CPC分类号： C07K7/56

摘要： A method for purifying a cyclic lipopepteide or a salt thereof is provided. The method comprises the steps: (1) extracting a fermentation broth containing a compound of formula I or a salt thereof, to obtain an extract 1 after filtration or centrifugation; (2) diluting or concentrating the extract 1 under vacuum to decrease the content of the organic solvent, to obtain an extract 2; (3) loading the extract 2 onto a macroporous absorption resin; (4) washing the macroporpous adsorption resin with water, an organic solvent, or a mixture of water and an organic solvent as a washing solution; and (5) eluting the compound of formula I off from the macroporous adsorption resin with water, an organic solvent, or a mixture of water and an organic solvent as a washing solution as an eluant. Compared with the prior art, the purification method has the advantages that fewer organic solvent is used, no silica gel is used, the harm to the environment is less, and the purity of the collected compound of formula I is improved.

摘要翻译： 提供了一种纯化环磷脂或其盐的方法。 该方法包括以下步骤：（1）提取含有式I化合物或其盐的发酵液，过滤或离心后得到提取物1; （2）真空稀释或浓缩提取物1以降低有机溶剂的含量，得到提取物2; （3）将提取物2装载到大孔吸附树脂上; （4）用水，有机溶剂或水和有机溶剂的混合物洗涤大孔吸附树脂作为洗涤溶液; 和（5）用水，有机溶剂或水和有机溶剂的混合物作为洗脱液从大孔吸附树脂中除去式I化合物作为洗脱剂。 与现有技术相比，纯化方法的优点是使用少量有机溶剂，不使用硅胶，对环境的危害较小，提取了所收集的式I化合物的纯度。

公开(公告)号：US08927690B2

公开(公告)日：2015-01-06

申请号：US13877427

申请日：2011-09-27

申请人： Zhaoli Zhang ,  Shidong Liu ,  Zhonghao Zhuo ,  Xiaoming Ji

发明人： Zhaoli Zhang ,  Shidong Liu ,  Zhonghao Zhuo ,  Xiaoming Ji

IPC分类号： A61K38/00 ,  C07K1/00 ,  C07K1/22 ,  C07K7/56

CPC分类号： C07K1/22 ,  C07K7/56

摘要： A process for purifying cyclic lipopeptide compounds or salts thereof comprising the steps of: (1) charging a crude compound of Formula I onto a macroporous adsorption resin; (2) washing the macroporous adsorption resin using water, an organic solvent or a mixed solution of an organic solvent and water as a washing liquid; and (3) eluting the compound of Formula I from the macroporous adsorption resin using water, an organic solvent or a mixed solution of an organic solvent and water as an eluent. The purification method has the advantages of using a small amount of organic solvents, using no silica gel, and causing little damage to the environment; the purity of the collected compound of formula I is also improved as compared with the methods previously disclosed.

摘要翻译： 一种用于纯化环状脂肽化合物或其盐的方法，包括以下步骤：（1）将式I的粗制化合物装入大孔吸附树脂中; （2）使用水，有机溶剂或有机溶剂和水的混合溶液作为洗涤液洗涤大孔吸附树脂; 和（3）使用水，有机溶剂或有机溶剂和水的混合溶液作为洗脱剂，从大孔吸附树脂中洗脱式I化合物。 净化方法的优点是使用少量的有机溶剂，不使用硅胶，对环境几乎没有损害; 与以前公开的方法相比，所收集的式I化合物的纯度也得到改善。

公开(公告)号：US08911968B2

公开(公告)日：2014-12-16

申请号：US13994633

申请日：2011-12-15

申请人： Yi Chen ,  Shidong Liu ,  Zhaoli Zhang ,  Chunxia Wang ,  Jing Kang ,  Xiaoming Ji

发明人： Yi Chen ,  Shidong Liu ,  Zhaoli Zhang ,  Chunxia Wang ,  Jing Kang ,  Xiaoming Ji

IPC分类号： C12P21/04 ,  C07K7/54 ,  C12R1/645 ,  C12N15/01 ,  C12N1/14 ,  C07K7/56

CPC分类号： C07K7/54 ,  C07K7/56 ,  C12N1/14 ,  C12N15/01 ,  C12R1/645

摘要： High-yield antibiotics producing strain, preparation method and use thereof are provided in the present invention. The high-yield strain is a mutagenized strain derived from Colephoma empetri, and deposited in CGMCC with the accession number of CGMCC 4129. The preparation method comprises the following steps: (a) mixing a seed liquid of Colephoma empetri of Accession No. FERM BP-2635 with nitrosoguanidine to obtain a mixture a; (b) mixing said mixture a with a wall-breaking enzyme to obtain protoplasts; (c) regenerating said protoplasts to obtain single colonies; and (d) culturing said single colonies to obtain said mutagenized strain. The obtained strain has stable genetic and producing property, produces little impurities in fermentation, and is suitable for industrialization.

摘要翻译： 本发明提供了高产抗生素生产菌株，其制备方法和用途。 高产菌株是来自科氏白菜的诱变菌株，保藏在CGMCC的CGMCC中，登录号为CGMCC 4129.该制备方法包括以下步骤：（a）将加入号为FERM BP的Colephoma empetri种子液 -2635与亚硝基胍混合得到混合物; （b）将所述混合物a与破壁酶混合以获得原生质体; （c）再生所述原生质体以获得单个菌落; 和（d）培养所述单个菌​​落以获得所述诱变菌株。 得到的菌株具有稳定的遗传和生产特性，发酵中杂质少，适用于工业化。

公开(公告)号：US20140162314A1

公开(公告)日：2014-06-12

申请号：US13994633

申请日：2011-12-15

申请人： Yi Chen ,  Shidong Liu ,  Zhaoli Zhang ,  Chunxia Wang ,  Jing Kang ,  Xiaoming Ji

发明人： Yi Chen ,  Shidong Liu ,  Zhaoli Zhang ,  Chunxia Wang ,  Jing Kang ,  Xiaoming Ji

IPC分类号： C07K7/54

CPC分类号： C07K7/54 ,  C07K7/56 ,  C12N1/14 ,  C12N15/01 ,  C12R1/645

摘要： High-yield antibiotics producing strain, preparation method and use thereof are provided in the present invention. The high-yield strain is a mutagenized strain derived from Colephoma empetri, and deposited in CGMCC with the accession number of CGMCC 4129. The preparation method comprises the following steps: (a) mixing a seed liquid of Colephoma empetri of Accession No. FERM BP-2635 with nitrosoguanidine to obtain a mixture a; (b) mixing said mixture a with a wall-breaking enzyme to obtain protoplasts; (c) regenerating said protoplasts to obtain single colonies; and (d) culturing said single colonies to obtain said mutagenized strain. The obtained strain has stable genetic and producing property, produces little impurities in fermentation, and is suitable for industrialization.

摘要翻译： 本发明提供了高产抗生素生产菌株，其制备方法和用途。 高产菌株是来自科氏白菜的诱变菌株，保藏在CGMCC的CGMCC中，登录号为CGMCC 4129.该制备方法包括以下步骤：（a）将加入号为FERM BP的Colephoma empetri种子液 -2635与亚硝基胍混合得到混合物; （b）将所述混合物a与破壁酶混合以获得原生质体; （c）再生所述原生质体以获得单个菌落; 和（d）培养所述单个菌​​落以获得所述诱变菌株。 得到的菌株具有稳定的遗传和生产特性，发酵中杂质少，适用于工业化。

公开(公告)号：US09546197B2

公开(公告)日：2017-01-17

申请号：US13982797

申请日：2012-01-31

申请人： Shidong Liu ,  Zhaoli Zhang ,  Yi Chen ,  Xiusheng Wang ,  Liangliang Zhou ,  Xiaoming Ji

发明人： Shidong Liu ,  Zhaoli Zhang ,  Yi Chen ,  Xiusheng Wang ,  Liangliang Zhou ,  Xiaoming Ji

IPC分类号： C07K7/54 ,  C07K7/56 ,  C12N1/14 ,  C12R1/645 ,  C12P7/64

CPC分类号： C07K7/54 ,  C07K7/56 ,  C12N1/14 ,  C12P7/64 ,  C12R1/645

摘要： Provided is a method for preparing a compound represented by Formula I or a salt thereof, wherein Coleophoma empetri F-11899 (FERM BP2635) and/or a mutant strain thereof is cultured in a medium containing amino acid or a salt thereof, an insoluble organic source, and a sugar alcohol, to produce the compound of Formula I or a salt thereof.

摘要翻译： 本发明提供了制备式I化合物或其盐的方法，其中，在含有氨基酸或其盐的培养基中培养含有绒毛母细胞F-11899（FERM BP2635）和/或其突变株的不溶性有机物 来源和糖醇，以制备式I化合物或其盐。

公开(公告)号：US08724765B2

公开(公告)日：2014-05-13

申请号：US13502477

申请日：2010-10-22

申请人： Yongbo Liu ,  Hongwei Zhang ,  Jian Li ,  Zhaoli Zhang ,  Liang Fan ,  Zhen Liu ,  Yutao Jia

发明人： Yongbo Liu ,  Hongwei Zhang ,  Jian Li ,  Zhaoli Zhang ,  Liang Fan ,  Zhen Liu ,  Yutao Jia

IPC分类号： H03D3/24

CPC分类号： H03L7/10 ,  H03L7/085 ,  H03L7/093

摘要： The present invention provides a locking method and system, and the method includes: a locking system performing a phase discrimination and conversion process to an input signal Fi of an external standard source and a feedback output signal F0 of a local thermostatic crystal oscillator which pass through a frequency division, to generate a clock signal clk and a signal sign which is used to denote a frequency size relationship between the signal Fi and the signal F0, and performing a filtering process to the signal clk and the signal sign, and performing a voltage controlled oscillation process to a signal ahead used to denote that the frequency of the signal F0 is lower than the frequency of the signal Fi and a signal lag used to denote that the frequency of the signal F0 is higher than the frequency of the signal Fi, to implement a locking of the signal F0 and the signal Fi.

摘要翻译： 本发明提供一种锁定方法和系统，并且该方法包括：对外部标准源的输入信号Fi和通过的本地恒温晶体振荡器的反馈输出信号F0执行相位鉴别和转换处理的锁定系统 分频，产生时钟信号clk和用于表示信号Fi和信号F0之间的频率大小关系的信号，并对信号clk和信号进行滤波处理，并执行电压 控制振荡处理到前面的信号，用于表示信号F0的频率低于信号Fi的频率，以及用于表示信号F0的频率高于信号Fi的频率的信号滞后， 以实现信号F0和信号Fi的锁定。