公开(公告)号：US20130034841A1

公开(公告)日：2013-02-07

申请号：US13565104

申请日：2012-08-02

申请人： Ronald C. Seubert ,  Jackie L. Stilwell ,  Paul Goodwin ,  Martha Stone ,  Arturo Bernardo Ramirez

发明人： Ronald C. Seubert ,  Jackie L. Stilwell ,  Paul Goodwin ,  Martha Stone ,  Arturo Bernardo Ramirez

IPC分类号： C12Q1/04 ,  C12M1/42 ,  C12M1/34 ,  C12Q1/70

CPC分类号： C12M47/04

摘要： Systems and methods for isolating and characterizing various target materials of a suspension are disclosed. A suspension suspected of containing the target materials is added to a tube. A float with a specific gravity corresponding to that of the target material is inserted into the tube. The tube, float, and suspension are centrifuged together causing the various materials suspended in the suspension to separate into different layers along the axial length of the tube according to their specific gravities. The float and/or tube are configured to drive the various target materials to a region of space between the float and inner wall of the tube.

摘要翻译： 公开了用于分离和表征悬浮液的各种靶材料的系统和方法。 将疑似含有目标物质的悬浮液加入管中。 将具有对应于目标材料的比重的浮子插入管中。 将管，浮子和悬浮液离心在一起，使得悬浮在悬浮液中的各种材料根据其比重沿管的轴向长度分离成不同的层。 浮子和/或管构造成将各种目标材料驱动到管的浮子和内壁之间的空间区域。

公开(公告)号：US20130029356A1

公开(公告)日：2013-01-31

申请号：US13558908

申请日：2012-07-26

申请人： Jackie L. Stilwell

发明人： Jackie L. Stilwell

IPC分类号： G01N21/64 ,  G01N21/78 ,  B82Y15/00

CPC分类号： G01N15/042 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2015/045

摘要： Methods and systems for using drugs as biomarkers to investigate the status of biological systems are disclosed. A drug is conjugated with a light emitting dye that emits light in a channel of the electromagnetic spectrum when an appropriate stimulus is applied. In one aspect, a suspension suspected of containing target particles is added to a tube along with the conjugated drug/dye complex and a float. Centrifugation of the tube, float, and suspension causes various components to separate along the axial length of the tube. Binding of the drug/dye complex to the target particles can be assessed by applying an appropriate stimulus to the tube, which, in turn, causes the fluorescent dyes to emit light in the channel. The level of fluorescence of the target particles located between the float and the inner wall of the tube can be used to assess use of the drug in therapy.

摘要翻译： 公开了使用药物作为生物标志物来研究生物系统状态的方法和系统。 当施加适当的刺激时，药物与发射染料共轭，所述发光染料在电磁光谱的通道中发光。 在一个方面，将疑似含有靶颗粒的悬浮液与缀合的药物/染料复合物和浮子一起加入到管中。 管，浮子和悬架的离心使各种部件沿着管的轴向长度分离。 可以通过对管施加适当的刺激来评估药物/染料复合物与靶颗粒的结合，这进而导致荧光染料在通道中发光。 位于浮子和管内壁之间的靶颗粒的荧光水平可用于评估药物在治疗中的用途。