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    Hydrophilic, high protein binding, low fluorescence, western blotting membrane
    5.
    发明授权
    Hydrophilic, high protein binding, low fluorescence, western blotting membrane 有权
    亲水，高蛋白结合，低荧光，蛋白质印迹膜

    公开(公告)号：US08143067B2

    公开(公告)日：2012-03-27

    申请号：US12938813

    申请日：2010-11-03

    申请人： Antoni Peters ,  Philip Goddard ,  John Charkoudian ,  Neil Soice ,  Dave Brewster ,  Anja Dedeo

    发明人： Antoni Peters ,  Philip Goddard ,  John Charkoudian ,  Neil Soice ,  Dave Brewster ,  Anja Dedeo

    IPC分类号： C12Q1/00 ,  G01N33/53 ,  C12P19/26 ,  B01D57/02

    CPC分类号： G01N33/54393 ,  B01D67/0093 ,  B01D2323/02 ,  B01D2323/30 ,  B01D2325/36 ,  Y10T436/24

    摘要： Hydrophilic membrane particularly suited for blotting applications, preferably Western blotting. A pre-wet hydrophobic membrane substrate, preferably made of PVDF, is contacted with a monomer solution and subjected to a UV-initiated free radical polymerization step to render the substrate permanently hydrophilic. The resulting membrane exhibits low background fluorescence, high protein binding, excellent retention of protein sample spot morphology, and extended dynamic range (high signal-to-noise ratio, enhanced sample detectability). The membrane demonstrates comparable or higher performance in Western blotting applications than conventional nitrocellulose Western blotting membranes, particularly for protein detection at low sample concentrations, and is directly water-wettable, eliminating the need for an alcohol pre-wet step prior to use.

    摘要翻译： 亲水膜特别适用于印迹应用，优选蛋白印迹法。 将优选由PVDF制成的预湿疏水膜基材与单体溶液接触并进行UV引发的自由基聚合步骤以使基材永久地亲水。 所得膜显示低背景荧光，高蛋白结合，优异的蛋白质样品斑点形态保留和扩展的动态范围（高信噪比，增强的样品检测性）。 与常规硝酸纤维素蛋白质印迹膜相比，该膜在Western印迹应用中表现出相当或更高的性能，特别是在低样品浓度下的蛋白质检测，并且是直接水可湿性的，在使用前无需使用酒精预湿步骤。

    Hydrophilic, High Protein Binding, Low Fluorescence, Western Blotting Membrane
    7.
    发明申请
    Hydrophilic, High Protein Binding, Low Fluorescence, Western Blotting Membrane 有权
    亲水，高蛋白结合，低荧光，Western印迹膜

    公开(公告)号：US20110045493A1

    公开(公告)日：2011-02-24

    申请号：US12938813

    申请日：2010-11-03

    申请人： Antoni Peters ,  Philip Goddard ,  John Charkoudian ,  Neil Soice ,  Dave Brewster ,  Anja Dedeo

    发明人： Antoni Peters ,  Philip Goddard ,  John Charkoudian ,  Neil Soice ,  Dave Brewster ,  Anja Dedeo

    IPC分类号： G01N33/53

    CPC分类号： G01N33/54393 ,  B01D67/0093 ,  B01D2323/02 ,  B01D2323/30 ,  B01D2325/36 ,  Y10T436/24

    摘要： Hydrophilic membrane particularly suited for blotting applications, preferably Western blotting. A pre-wet hydrophobic membrane substrate, preferably made of PVDF, is contacted with a monomer solution and subjected to a UV-initiated free radical polymerization step to render the substrate permanently hydrophilic. The resulting membrane exhibits low background fluorescence, high protein binding, excellent retention of protein sample spot morphology, and extended dynamic range (high signal-to-noise ratio, enhanced sample detectability). The membrane demonstrates comparable or higher performance in Western blotting applications than conventional nitrocellulose Western blotting membranes, particularly for protein detection at low sample concentrations, and is directly water-wettable, eliminating the need for an alcohol pre-wet step prior to use.

    摘要翻译： 亲水膜特别适用于印迹应用，优选蛋白印迹法。 将优选由PVDF制成的预湿疏水膜基材与单体溶液接触并进行UV引发的自由基聚合步骤以使基材永久地亲水。 所得膜显示低背景荧光，高蛋白结合，优异的蛋白质样品斑点形态保留和扩展的动态范围（高信噪比，增强的样品检测性）。 与常规硝酸纤维素蛋白质印迹膜相比，该膜在Western印迹应用中表现出相当或更高的性能，特别是在低样品浓度下的蛋白质检测，并且是直接水可湿性的，在使用前无需使用酒精预湿步骤。

    Hydrophilic, high protein binding, low fluorescence, western blotting membrane
    10.
    发明申请
    Hydrophilic, high protein binding, low fluorescence, western blotting membrane 有权
    亲水，高蛋白结合，低荧光，蛋白质印迹膜

    公开(公告)号：US20100044302A1

    公开(公告)日：2010-02-25

    申请号：US12462635

    申请日：2009-08-06

    申请人： Antoni Peters ,  Philip Goddard ,  John Charkoudian ,  Neil Soice ,  Dave Brewster ,  Anja Dedeo

    发明人： Antoni Peters ,  Philip Goddard ,  John Charkoudian ,  Neil Soice ,  Dave Brewster ,  Anja Dedeo

    IPC分类号： B01D71/40 ,  B01D71/34 ,  B05D3/02

    CPC分类号： G01N33/54393 ,  B01D67/0093 ,  B01D2323/02 ,  B01D2323/30 ,  B01D2325/36 ,  Y10T436/24

    摘要： Hydrophilic membrane particularly suited for blotting applications, preferably Western blotting. A pre-wet hydrophobic membrane substrate, preferably made of PVDF, is contacted with a monomer solution and subjected to a UV-initiated free radical polymerization step to render the substrate permanently hydrophilic. The resulting membrane exhibits low background fluorescence, high protein binding, excellent retention of protein sample spot morphology, and extended dynamic range (high signal-to-noise ratio, enhanced sample detectability). The membrane demonstrates comparable or higher performance in Western blotting applications than conventional nitrocellulose Western blotting membranes, particularly for protein detection at low sample concentrations, and is directly water-wettable, eliminating the need for an alcohol pre-wet step prior to use.

    摘要翻译： 亲水膜特别适用于印迹应用，优选蛋白印迹法。 将优选由PVDF制成的预湿疏水膜基材与单体溶液接触并进行UV引发的自由基聚合步骤以使基材永久地亲水。 所得膜显示低背景荧光，高蛋白结合，优异的蛋白质样品斑点形态保留和扩展的动态范围（高信噪比，增强的样品检测性）。 与常规硝酸纤维素蛋白质印迹膜相比，该膜在Western印迹应用中表现出相当或更高的性能，特别是在低样品浓度下的蛋白质检测，并且是直接水可湿性的，在使用前无需使用酒精预湿步骤。