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    Methods for purifying a target protein from one or more impurities in a sample
    7.
    发明授权
    Methods for purifying a target protein from one or more impurities in a sample 有权
    从样品中的一种或多种杂质纯化靶蛋白的方法

    公开(公告)号：US08536316B2

    公开(公告)日：2013-09-17

    申请号：US12851082

    申请日：2010-08-05

    申请人： Neil Soice ,  John Dana Hubbard ,  Yu Zhang ,  James Hamzik

    发明人： Neil Soice ,  John Dana Hubbard ,  Yu Zhang ,  James Hamzik

    IPC分类号： A23J1/00 ,  C07K1/00

    CPC分类号： C07K1/36 ,  C07K16/00

    摘要： The present invention relates, at least in part, to improved methods of protein purification. In particular, the present invention relates, at least in part, to methods for purifying an Fc region containing protein from a composition comprising the Fc region containing protein and one or more impurities, where the methods eliminate the need for a holding tank and/or a buffer exchange step.

    摘要翻译： 本发明至少部分地涉及改进的蛋白质纯化方法。 特别地，本发明至少部分涉及从含有Fc区蛋白质和一种或多种杂质的组合物中纯化含有Fc区的蛋白质的方法，其中该方法不需要储存罐和/或 缓冲交换步骤。

    Hydrophilic, high protein binding, low fluorescence, western blotting membrane
    8.
    发明授权
    Hydrophilic, high protein binding, low fluorescence, western blotting membrane 有权
    亲水，高蛋白结合，低荧光，蛋白质印迹膜

    公开(公告)号：US08143067B2

    公开(公告)日：2012-03-27

    申请号：US12938813

    申请日：2010-11-03

    申请人： Antoni Peters ,  Philip Goddard ,  John Charkoudian ,  Neil Soice ,  Dave Brewster ,  Anja Dedeo

    发明人： Antoni Peters ,  Philip Goddard ,  John Charkoudian ,  Neil Soice ,  Dave Brewster ,  Anja Dedeo

    IPC分类号： C12Q1/00 ,  G01N33/53 ,  C12P19/26 ,  B01D57/02

    CPC分类号： G01N33/54393 ,  B01D67/0093 ,  B01D2323/02 ,  B01D2323/30 ,  B01D2325/36 ,  Y10T436/24

    摘要： Hydrophilic membrane particularly suited for blotting applications, preferably Western blotting. A pre-wet hydrophobic membrane substrate, preferably made of PVDF, is contacted with a monomer solution and subjected to a UV-initiated free radical polymerization step to render the substrate permanently hydrophilic. The resulting membrane exhibits low background fluorescence, high protein binding, excellent retention of protein sample spot morphology, and extended dynamic range (high signal-to-noise ratio, enhanced sample detectability). The membrane demonstrates comparable or higher performance in Western blotting applications than conventional nitrocellulose Western blotting membranes, particularly for protein detection at low sample concentrations, and is directly water-wettable, eliminating the need for an alcohol pre-wet step prior to use.

    摘要翻译： 亲水膜特别适用于印迹应用，优选蛋白印迹法。 将优选由PVDF制成的预湿疏水膜基材与单体溶液接触并进行UV引发的自由基聚合步骤以使基材永久地亲水。 所得膜显示低背景荧光，高蛋白结合，优异的蛋白质样品斑点形态保留和扩展的动态范围（高信噪比，增强的样品检测性）。 与常规硝酸纤维素蛋白质印迹膜相比，该膜在Western印迹应用中表现出相当或更高的性能，特别是在低样品浓度下的蛋白质检测，并且是直接水可湿性的，在使用前无需使用酒精预湿步骤。