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    Method and apparatus for making porous agarose beads
    8.
    发明申请
    Method and apparatus for making porous agarose beads 有权
    制备多孔琼脂糖珠的方法和设备

    公开(公告)号：US20070069408A1

    公开(公告)日：2007-03-29

    申请号：US11520775

    申请日：2006-09-13

    申请人： Kwok-Shun Cheng ,  Senthilkumar Ramaswamy ,  Nanying Bian ,  Brian Gagnon ,  Joaquin Umana ,  Neil Soice

    发明人： Kwok-Shun Cheng ,  Senthilkumar Ramaswamy ,  Nanying Bian ,  Brian Gagnon ,  Joaquin Umana ,  Neil Soice

    IPC分类号： B01J13/04 ,  B28B1/54

    CPC分类号： B01J20/24 ,  B01J13/04 ,  B01J20/28009 ,  B01J20/285 ,  B01J20/286 ,  B01J20/291 ,  B01J20/3204 ,  B01J20/3242 ,  B01J20/3244 ,  B01J20/3272 ,  B01J20/328 ,  B01J20/3282 ,  B01J20/3289 ,  B01J20/3293 ,  B01J2220/4825

    摘要： The present invention relates to a method and apparatus for forming agarose or cored agarose beads. The process involves dissolving/gelation the agarose in a suitable liquid, mixing it with a hydrophobic liquid to form an emulsion and maintaining that emulsion at a temperature equal to or greater than the gelation point of the agarose, passing it through a static mixer to create agarose droplets and solidifying the agarose droplets in a second bath of hydrophobic liquid. The beads can then be washed and used or further processed to crosslink the agarose and/ or add various functionalities on to the agarose. Another method for solidifying the agarose droplets is by using a heat exchanger to cool the stream continuously after it exits the static mixer. A similar process is used for the “cored” beads except cores, preferably in bead form, are introduced to the agarose before it enters the first hydrophobic liquid so that the agarose forms a coating on the cores. A similar process with either agarose beads (made by this or another process) or cored agarose (made by this or another process) can be used to add multiple layers of agarose on to the existing beads. An apparatus for running the process is also disclosed.

    摘要翻译： 本发明涉及用于形成琼脂糖或核心琼脂糖珠的方法和装置。 该方法包括将琼脂糖溶解/凝胶化在合适的液体中，将其与疏水性液体混合以形成乳液，并将该乳液保持在等于或大于琼脂糖凝胶点的温度，将其通过静态混合器以产生 琼脂糖滴并在第二浴疏水性液体中固化琼脂糖滴。 然后可以将珠子洗涤并使用或进一步加工以使琼脂糖交联和/或在琼脂糖上添加各种功能。 用于固化琼脂糖滴的另一种方法是使用热交换器在离开静态混合器之后连续冷却流。 对于“核心”珠粒，使用类似的方法，除了核心，优选以珠粒形式，在其进入第一疏水性液体之前将其引入琼脂糖，使得琼脂糖在芯上形成涂层。 可以使用与琼脂糖珠（由该方法或另一方法制备）或核心琼脂糖（由该方法或另一方法制备）相似的方法将多层琼脂糖添加到现有珠粒上。 还公开了一种用于运行该过程的装置。

    Method and apparatus for making porous agarose beads
    10.
    发明授权
    Method and apparatus for making porous agarose beads 有权
    制备多孔琼脂糖珠的方法和设备

    公开(公告)号：US07678302B2

    公开(公告)日：2010-03-16

    申请号：US11520775

    申请日：2006-09-13

    申请人： Kwok-Shun Cheng ,  Senthilkumar Ramaswamy ,  Nanying Bian ,  Brian Gagnon ,  Joaquin Umana ,  Neil P. Soice

    发明人： Kwok-Shun Cheng ,  Senthilkumar Ramaswamy ,  Nanying Bian ,  Brian Gagnon ,  Joaquin Umana ,  Neil P. Soice

    IPC分类号： B29B9/00

    CPC分类号： B01J20/24 ,  B01J13/04 ,  B01J20/28009 ,  B01J20/285 ,  B01J20/286 ,  B01J20/291 ,  B01J20/3204 ,  B01J20/3242 ,  B01J20/3244 ,  B01J20/3272 ,  B01J20/328 ,  B01J20/3282 ,  B01J20/3289 ,  B01J20/3293 ,  B01J2220/4825

    摘要： The present invention relates to a method and apparatus for forming agarose or cored agarose beads. The process involves dissolving/gelation the agarose in a suitable liquid, mixing it with a hydrophobic liquid to form an emulsion and maintaining that emulsion at a temperature equal to or greater than the gelation point of the agarose, passing it through a static mixer to create agarose droplets and solidifying the agarose droplets in a second bath of hydrophobic liquid. The beads can then be washed and used or further processed to crosslink the agarose and/or add various functionalities on to the agarose. Another method for solidifying the agarose droplets is by using a heat exchanger to cool the stream continuously after it exits the static mixer. A similar process is used for the “cored” beads except cores, preferably in bead form, are introduced to the agarose before it enters the first hydrophobic liquid so that the agarose forms a coating on the cores. A similar process with either agarose beads (made by this or another process) or cored agarose (made by this or another process) can be used to add multiple layers of agarose on to the existing beads. An apparatus for running the process is also disclosed.

    摘要翻译： 本发明涉及用于形成琼脂糖或核心琼脂糖珠的方法和装置。 该方法包括将琼脂糖溶解/凝胶化在合适的液体中，将其与疏水性液体混合以形成乳液，并将该乳液保持在等于或大于琼脂糖凝胶点的温度，将其通过静态混合器以产生 琼脂糖滴并在第二浴疏水性液体中固化琼脂糖滴。 然后可以将珠子洗涤并使用或进一步加工以使琼脂糖交联和/或在琼脂糖上添加各种功能。 用于固化琼脂糖滴的另一种方法是通过使用热交换器在离开静态混合器之后连续冷却流。 对于“核心”珠粒，使用类似的方法，除了核心，优选以珠粒形式，在其进入第一疏水性液体之前将其引入琼脂糖，使得琼脂糖在芯上形成涂层。 可以使用与琼脂糖珠（由该方法或另一方法制备）或核心琼脂糖（由该方法或另一方法制备）相似的方法将多层琼脂糖添加到现有珠粒上。 还公开了一种用于运行该过程的装置。