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公开(公告)号：US20120178167A1
公开(公告)日：2012-07-12
申请号：US13426422
申请日：2012-03-21
申请人： James Eberwine , Philip G. Haydon , Jai-Yoon Sul , Hajime Takano , Chia-Wen Kitty Wu , Fanyi Zeng
发明人： James Eberwine , Philip G. Haydon , Jai-Yoon Sul , Hajime Takano , Chia-Wen Kitty Wu , Fanyi Zeng
IPC分类号： C12N15/87
CPC分类号： A61K48/0083 , C12N13/00 , C12N15/1079 , C12N15/87
摘要： The present invention includes methods for transferring a multigenic phenotype to a cell by transfecting, preferably by phototransfection, and locally transfecting a cell or a cellular process with a laser while the cell is bathed in a fluid medium comprising two or more nucleic acids, thereby introducing the nucleic acid into the interior of the cell. Expression of the nucleic acids results in a multigenic phenotype in the tranfected cell.
摘要翻译： 本发明包括通过转染优选通过光转染，以及当细胞浸泡在包含两种或更多种核酸的流体培养基中时用激光局部转染细胞或细胞过程将多基因表型转移到细胞的方法，从而引入 核酸进入细胞内部。 核酸的表达在转染的细胞中导致多基因表型。
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公开(公告)号：US10646590B2
公开(公告)日：2020-05-12
申请号：US12086471
申请日：2006-12-12
申请人： James Eberwine , Philip G. Haydon , Jai-Yoon Sul , Hajime Takano , Chia-Wen Kitty Wu , Fanyi Zeng
发明人： James Eberwine , Philip G. Haydon , Jai-Yoon Sul , Hajime Takano , Chia-Wen Kitty Wu , Fanyi Zeng
摘要： The present invention includes methods for transferring a multigenic phenotype to a cell by transfecting, preferably by phototransfection, and locally transfecting a cell or a cellular process with a laser while the cell is bathed in a fluid medium comprising two or more nucleic acids, thereby introducing the nucleic acid into the interior of the cell. Expression of the nucleic acids results in a multigenic phenotype in the transfected cell.
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公开(公告)号：US20120135493A1
公开(公告)日：2012-05-31
申请号：US12086471
申请日：2006-12-12
申请人： James Eberwine , Philip G. Haydon , Jai-Yoon Sul , Hajime Takano , Chia-Wen Kitty Wu , Fanyi Zeng
发明人： James Eberwine , Philip G. Haydon , Jai-Yoon Sul , Hajime Takano , Chia-Wen Kitty Wu , Fanyi Zeng
CPC分类号： A61K48/0083 , C12N13/00 , C12N15/1079 , C12N15/87
摘要： The present invention includes methods for transferring a multigenic phenotype to a cell by transfecting, preferably by phototransfection, and locally transfecting a cell or a cellular process with a laser while the cell is bathed in a fluid medium comprising two or more nucleic acids, thereby introducing the nucleic acid into the interior of the cell. Expression of the nucleic acids results in a multigenic phenotype in the tranfected cell.
摘要翻译： 本发明包括通过转染优选通过光转染，以及当细胞浸泡在包含两种或更多种核酸的流体培养基中时用激光局部转染细胞或细胞过程将多基因表型转移到细胞的方法，从而引入 核酸进入细胞内部。 核酸的表达在转染的细胞中导致多基因表型。
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公开(公告)号：US10583204B2
公开(公告)日：2020-03-10
申请号：US13426422
申请日：2012-03-21
申请人： James Eberwine , Philip G. Haydon , Jai-Yoon Sul , Hajime Takano , Chia-Wen Kitty Wu , Fanyi Zeng
发明人： James Eberwine , Philip G. Haydon , Jai-Yoon Sul , Hajime Takano , Chia-Wen Kitty Wu , Fanyi Zeng
摘要： The present invention includes methods for transferring a multigenic phenotype to a cell by transfecting, preferably by phototransfection, and locally transfecting a cell or a cellular process with a laser while the cell is bathed in a fluid medium comprising two or more nucleic acids, thereby introducing the nucleic acid into the interior of the cell. Expression of the nucleic acids results in a multigenic phenotype in the tranfected cell.
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公开(公告)号：US20110033934A1
公开(公告)日：2011-02-10
申请号：US12755277
申请日：2010-04-06
申请人： James Eberwine , Jai-Yoon Sul , Chia-Wen Wu , Fanyi Zeng , Junhyong Kim
发明人： James Eberwine , Jai-Yoon Sul , Chia-Wen Wu , Fanyi Zeng , Junhyong Kim
CPC分类号： C12N5/062 , C12N5/0622 , C12N5/0657 , C12N2501/998 , C12N2506/00 , C12N2506/08 , C12N2506/1307
摘要： The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.
摘要翻译： 本发明包括通过用表型转化核酸转染细胞来实现细胞中表型转化的方法。 核酸的表达导致转染细胞中的表型转化。 表型转换核酸优选是转录组，更优选mRNA转录组。
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公开(公告)号：US10647960B2
公开(公告)日：2020-05-12
申请号：US12755277
申请日：2010-04-06
申请人： James Eberwine , Jai-Yoon Sul , Chia-Wen Wu , Fanyi Zeng , Junhyong Kim
发明人： James Eberwine , Jai-Yoon Sul , Chia-Wen Wu , Fanyi Zeng , Junhyong Kim
IPC分类号： A61K48/00 , C07H21/02 , C12N5/071 , C12N5/00 , C12N15/87 , C12N5/0793 , C12N5/079 , C12N5/077 , C12Q1/68
摘要： The present invention includes methods for effecting phenotype conversion in a cell by transfecting the cell with phenotype-converting nucleic acid. Expression of the nucleic acids results in a phenotype conversion in the transfected cell. Preferably the phenotype-converting nucleic acid is a transcriptome, and more preferably an mRNA transcriptome.
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