公开(公告)号：US5776772A

公开(公告)日：1998-07-07

申请号：US593865

申请日：1996-01-30

申请人： James G. Paulson ,  Eryn Ujita-Lee ,  Karen J. Colley ,  Beverly Adler ,  Jeffrey K. Browne ,  Jasminder Weinstein

发明人： James G. Paulson ,  Eryn Ujita-Lee ,  Karen J. Colley ,  Beverly Adler ,  Jeffrey K. Browne ,  Jasminder Weinstein

IPC分类号： C12N9/10 ,  C12N15/54 ,  C12P19/18 ,  C12P19/26 ,  C12N15/63 ,  C12N15/00 ,  C12N15/79

CPC分类号： C12P19/18 ,  C12N9/1051 ,  C12P19/26

摘要： A method for genetically engineering cells to produce soluble and secretable Golgi processing enzymes instead of naturally occurring membrane-bound enzymes. Cells are genetically engineered to express glycosyltransferases which lack both a membrane anchor and a retention signal. The resulting altered enzyme becomes soluble and secretable by the cell without losing its catalytic activity. Secretion of the soluble glycosyltransferase by the cell provides for increased production and simplified recovery of glycosyltransferase.

公开(公告)号：US5541083A

公开(公告)日：1996-07-30

申请号：US209604

申请日：1994-03-10

申请人： James C. Paulson ,  Eryn Ujita-Lee ,  Karen J. Colley ,  Beverly Adler ,  Jeffrey K. Browne ,  Jasminder Weinstein

发明人： James C. Paulson ,  Eryn Ujita-Lee ,  Karen J. Colley ,  Beverly Adler ,  Jeffrey K. Browne ,  Jasminder Weinstein

IPC分类号： C12N9/10 ,  C12N15/54 ,  C12P19/18 ,  C12P19/26 ,  C12N15/52 ,  C12P1/00 ,  C12P21/06

CPC分类号： C12P19/18 ,  C12N9/1051 ,  C12P19/26

摘要： A method for genetically engineering cells to produce soluble and secretable Golgi processing enzymes instead of naturally occurring membrane-bound enzymes. Cells are genetically engineered to express glycosyltransferases which lack both a membrane anchor and a retention signal. The resulting altered enzyme becomes soluble and secretable by the cell without losing its catalytic activity. Secretion of the soluble glycosyltransferase by the cell provides for increased production and simplified recovery of glycosyltransferase.

摘要翻译： 一种用于遗传工程细胞产生可溶性和可分泌的高尔基体加工酶而不是天然存在的膜结合酶的方法。 细胞被遗传工程化以表达缺少膜锚定和保留信号的糖基转移酶。 所得到的改变的酶变得可溶并且可被细胞分泌而不失去其催化活性。 由细胞分泌可溶性糖基转移酶提供增加的糖基转移酶的产生和简化的恢复。

公开(公告)号：US5032519A

公开(公告)日：1991-07-16

申请号：US426577

申请日：1989-10-24

申请人： James C. Paulson ,  Eryn Ujita-Lee ,  Karen J. Colley ,  Beverly Adler ,  Jeffrey K. Browne

发明人： James C. Paulson ,  Eryn Ujita-Lee ,  Karen J. Colley ,  Beverly Adler ,  Jeffrey K. Browne

IPC分类号： C12N9/10 ,  C12N15/09 ,  C12N15/54 ,  C12N15/55 ,  C12P19/18 ,  C12P19/26 ,  C12R1/91

CPC分类号： C12N9/1051 ,  C12P19/18 ,  C12P19/26

摘要： A method for genetically engineering cells to produce soluble and secretable Golgi processing enzymes instead of naturally occurring membrane-bound enzymes. Cells are genetically engineered to express glycosyltransferases which lack both a membrane anchor and a rThis invention was made with government support under Grant Contract Nos. GM-27904 and GM-11557 awarded by the National Institute of Health. The government has certain rights in this invention. The publications and other reference materials referred to herein to describe the background of the invention and to provide additional detail regarding its practice are hereby incorporated by reference. For convenience, the reference materials are numerically referenced and grouped in the appended bibliography.

摘要翻译： 一种用于遗传工程细胞产生可溶性和可分泌的高尔基体加工酶而不是天然存在的膜结合酶的方法。 细胞被遗传工程化以表达缺少膜锚定和保留信号的糖基转移酶。 所得到的改变的酶变得可溶并且可被细胞分泌而不失去其催化活性。 由细胞分泌可溶性糖基转移酶提供增加的糖基转移酶的产生和简化的恢复。