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公开(公告)号:US07534623B2
公开(公告)日:2009-05-19
申请号:US10517980
申请日:2003-06-11
申请人: James P. Landers , Pamela E. Norris , Mary E. Power , Jerome P. Ferrance , Sushil Shrinivasan , Kelley A. Wolfe , Michael C. Breadmore
发明人: James P. Landers , Pamela E. Norris , Mary E. Power , Jerome P. Ferrance , Sushil Shrinivasan , Kelley A. Wolfe , Michael C. Breadmore
CPC分类号: C12Q1/6806 , B01J20/103 , B01L3/5027 , B01L3/502753 , B01L2200/0631 , G01N1/405 , G01N30/6095 , G01N2030/009 , G01N2030/524 , G01N2035/00158 , G01N2035/00366 , Y10T436/25375 , C12Q2565/629
摘要: The present invention is directed to novel device comprising a sol-gel filled microchannel and methods for purifying nucleic acids from biological samples. In one embodiment shown in FIG. 1, the microfluidic device (1) comprises a base (2) with a microchannel (3) formed in the interior of base (2), wherein said microchannel (3) is filled with a sol-gel matrix and in fluid communication with an inlet port (4) and outlet port (5) wherein inlet port (4) and outlet port (5) are formed on the exterior surface (10) of base (2). The device may be further provided with additional components to allow for analytical analysis of the purified nucleic acid sequences.
摘要翻译: 本发明涉及包含溶胶 - 凝胶填充微通道和从生物样品中纯化核酸的方法的新型装置。 在图1所示的一个实施例中, 如图1所示,微流体装置(1)包括具有形成在基部(2)内部的微通道(3)的基部(2),其中所述微通道(3)填充有溶胶 - 凝胶基质并与 入口(4)和出口(5),其中入口(4)和出口(5)形成在基座(2)的外表面(10)上。 该装置可以进一步提供另外的组分以允许对纯化的核酸序列进行分析分析。