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    Stabilized fluorescent rare earth labels and labeled physiologically reactive species
    1.
    发明授权
    Stabilized fluorescent rare earth labels and labeled physiologically reactive species 失效
    稳定的荧光稀土标签和标记的生理反应物种

    公开(公告)号：US4735907A

    公开(公告)日：1988-04-05

    申请号：US713202

    申请日：1985-03-18

    申请人： James R. Schaeffer ,  Tsang J. Chen ,  Michael A. Schen

    发明人： James R. Schaeffer ,  Tsang J. Chen ,  Michael A. Schen

    IPC分类号： G01N21/76 ,  C08F8/00 ,  C08G81/00 ,  C08G81/02 ,  G01N33/533 ,  G01N33/543 ,  G01N33/545 ,  G01N33/58 ,  G01N33/546

    CPC分类号： G01N33/585 ,  G01N33/533 ,  Y10S436/80 ,  Y10S436/805 ,  Y10S436/808 ,  Y10T428/2982

    摘要： Improved fluorescent labels comprise a fluorescent rare earth chelate incorporated into a polymeric particle derived from a loadable latex. The polymer comprises: (a) from about 50 to about 96 weight percent of recurring units derived from a hydrophobic ethylenically unsaturated polymerizable monomer, (b) from about 2 to about 30 weight percent of recurring units derived from a nonionic hydrophilic ethylenically unsaturated polymerizable monomer, and (c) from about 2 to about 20 weight percent of recurring units derived from an anionic ethylenically unsaturated polymerizable monomer containing at least one solubilizing group. The labels of this invention have greatly improved stability in aqueous solutions and do not prematurely agglomerate during storage. They can be attached to any of a variety of physiologically reactive species to provide labeled species, and are particularly useful in specific binding assays.

    摘要翻译： 改进的荧光标记包括掺入衍生自可加载胶乳的聚合物颗粒中的荧光稀土螯合物。 聚合物包含：（a）约50至约96重量％的衍生自疏水性烯属不饱和可聚合单体的重复单元，（b）约2至约30重量％的衍生自非离子亲水烯属不饱和可聚合单体的重复单元 ，和（c）约2-约20重量％的衍生自含有至少一个增溶基团的阴离子烯属不饱和可聚合单体的重复单元。 本发明的标签大大提高了水溶液的稳定性，并且在储存期间不会过早聚集。 它们可以连接到各种生理反应性物质中的任一种以提供标记的物质，并且在特异性结合测定中特别有用。

    Latex particles incorporating stabilized fluorescent rare earth labels
    2.
    发明授权
    Latex particles incorporating stabilized fluorescent rare earth labels 失效
    掺入稳定荧光稀土标签的乳胶颗粒

    公开(公告)号：US4784912A

    公开(公告)日：1988-11-15

    申请号：US112302

    申请日：1987-10-26

    申请人： James R. Schaeffer ,  Tsang J. Chen ,  Michael A. Schen

    发明人： James R. Schaeffer ,  Tsang J. Chen ,  Michael A. Schen

    IPC分类号： G01N21/76 ,  C08F8/00 ,  C08G81/00 ,  C08G81/02 ,  G01N33/533 ,  G01N33/543 ,  G01N33/545 ,  G01N33/58 ,  B32B27/32 ,  G01N33/546

    CPC分类号： G01N33/585 ,  G01N33/533 ,  Y10S436/80 ,  Y10S436/805 ,  Y10S436/808 ,  Y10T428/2982

    摘要： Improved fluorescent labels comprise a fluorescent rare earth chelate incorporated into a polymeric particle derived from a loadable latex. The polymer comprises: (a) from about 50 to about 96 weight percent of recurring units derived from a hydrophobic ethylenically unsaturated polymerizable monomer, (b) from about 2 to about 30 weight percent of recurring units derived from a nonionic hydrophilic ethylenically unsaturated polymerizable monomer, and (c) from about 2 to about 20 weight percent of recurring units derived from an anionic ethylenically unsaturated polymerizable monomer containing at least one solubilizing group. The labels of this invention have greatly improved stability in aqueous solutions and do not prematurely agglomerate during storage. They can be attached to any of a variety of physiologically reactive species to provide labeled species, and are particularly useful in specific binding assays.

    摘要翻译： 改进的荧光标记包括掺入衍生自可加载胶乳的聚合物颗粒中的荧光稀土螯合物。 聚合物包含：（a）约50至约96重量％的衍生自疏水性烯属不饱和可聚合单体的重复单元，（b）约2至约30重量％的衍生自非离子亲水烯属不饱和可聚合单体的重复单元 ，和（c）约2-约20重量％的衍生自含有至少一个增溶基团的阴离子烯属不饱和可聚合单体的重复单元。 本发明的标签大大提高了水溶液的稳定性，并且在储存期间不会过早聚集。 它们可以连接到各种生理反应性物质中的任一种以提供标记的物质，并且在特异性结合测定中特别有用。

    Analytical element for clinical analysis
    5.
    发明授权
    Analytical element for clinical analysis 失效
    临床分析分析要素

    公开(公告)号：US4110079A

    公开(公告)日：1978-08-29

    申请号：US805215

    申请日：1977-06-09

    申请人： James R. Schaeffer ,  Louis M. Minsk ,  Richard E. Stevens

    发明人： James R. Schaeffer ,  Louis M. Minsk ,  Richard E. Stevens

    IPC分类号： G01N33/52 ,  G01N31/22

    CPC分类号： G01N33/525

    摘要： An analytical element for use in the clinical assay of an analyte contained in a biological liquid sample which is applied from an external source to a substantially planar sample receiving suface of the element. The analytical element comprises a porous layer bearing the aforementioned sample receiving surface; at least one dry reagent material for use in the assay; and one or more associated rupturable pod-like member(s) containing a liquid reagent for the assay, such liquid reagent, upon rupture of the pod-like member(s), being released into the element.

    摘要翻译： 用于生物液体样品中包含的分析物的临床测定中的分析元件，其从外部源施加到接收该元件表面的基本上平面的样品。 分析元件包括承载上述样品接收表面的多孔层; 用于测定中的至少一种干试剂材料; 和一个或多个相关联的可破裂的荚状构件，其包含用于所述测定的液体试剂，所述液体试剂在所述荚状构件破裂时被释放到所述元件中。

    Dry analytical element for acetaminophen assay
    6.
    发明授权
    Dry analytical element for acetaminophen assay 失效
    对乙酰氨基酚测定用干燥分析元素

    公开(公告)号：US06783731B1

    公开(公告)日：2004-08-31

    申请号：US08493442

    申请日：1995-06-22

    申请人： Thomas C. Arter ,  John C. Mauck ,  James R. Schaeffer ,  Robert F. Winterkorn

    发明人： Thomas C. Arter ,  John C. Mauck ,  James R. Schaeffer ,  Robert F. Winterkorn

    IPC分类号： G01N2100

    CPC分类号： C12Q1/34 ,  G01N2333/98 ,  Y10S435/966 ,  Y10S435/969 ,  Y10S435/97 ,  Y10S436/815

    摘要： A spectrophotometric assay for the detection of acetaminophen in aqueous fluids is carried out with a dry analytical element. The element comprises a support having thereon one or more reagent layers containing a first enzyme, aryl acylamidase, to cleave the amide bond of acetaminophen to produce p-aminophenol; and a mild oxidizing agent to oxidize the p-aminophenol so that it couples to a water-soluble coupling agent to form a dye that is read at 670 nm. The assay is precise, accurate on serum and plasma samples, and relatively free from significant interferences. The element also allows measurement over a broad dynamic range.

    摘要翻译： 用水分液体检测对乙酰氨基酚的分光光度法用干燥分析元件进行。 元件包括其上具有一个或多个试剂层的载体，其含有第一种酶，芳基酰胺酶，以切割对乙酰氨基酚的酰胺键以产生对氨基苯酚; 和一种温和的氧化剂，以氧化对氨基苯酚，使其与水溶性偶联剂偶联形成在670nm读取的染料。 该测定法在血清和血浆样品中精确，准确，并且相对没有显着的干扰。 该元件还允许在宽动态范围内进行测量。