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26 条记录 (耗时 0.019 秒)

    Lower alcohol sulfate wash solution
    1.
    发明授权
    Lower alcohol sulfate wash solution 失效
    低级酒精硫酸盐洗液

    公开(公告)号：US5599715A

    公开(公告)日：1997-02-04

    申请号：US546980

    申请日：1990-07-02

    申请人： Harold C. Warren, III ,  Norbert S. Norkus ,  Margaret J. Smith-Lewis

    发明人： Harold C. Warren, III ,  Norbert S. Norkus ,  Margaret J. Smith-Lewis

    IPC分类号： G01N33/531 ,  G01N33/537 ,  G01N33/76 ,  G01N33/53

    CPC分类号： G01N33/76 ,  G01N33/537 ,  Y10S435/81 ,  Y10S435/975 ,  Y10S436/808 ,  Y10S436/811 ,  Y10T436/107497

    摘要： An aqueous wash solution is buffered to a pH of from about 5 to about 9 and contains at least about 1.5 weight percent of a compound comprising a lower alcohol sulfate anion having from 6 to 10 carbon atoms and an alkali metal or ammonium cation, such as sodium decyl sulfate. This wash solution is useful in a method for the determination of an immunological ligand, and is not prone to crystallization at lower temperatures. Particularly, it is useful for washing the immunological complex formed between the ligand and a receptor molecule therefor. Unreacted materials can be readily separated from the complex by the washing, particularly if the separation is carried out using a filtration membrane in a test device. A test kit for ligand determination comprises the wash solution as well as one or more receptors for the ligand, at least one of which is labeled for detection. This kit is particularly useful for measuring human chorionic gonadotropin (hCG) as an early indicator of pregnancy.

    摘要翻译： 将水性洗涤溶液缓冲至约5至约9的pH，并含有至少约1.5重量％的包含具有6至10个碳原子的低级醇硫酸根阴离子和碱金属或铵阳离子的化合物，例如 十二烷基硫酸钠。 该洗涤溶液可用于测定免疫配体的方法，并且在较低温度下不易于结晶。 特别地，用于洗涤配体和受体分子之间形成的免疫复合物是有用的。 未反应的材料可以通过洗涤容易地从络合物中分离，特别是如果在测试装置中使用过滤膜进行分离。 用于配体测定的测试试剂盒包括洗涤溶液以及配体的一种或多种受体，其中至少一种被标记用于检测。 该试剂盒特别适用于测量人绒毛膜促性腺激素（hCG）作为怀孕的早期指标。

    Nucleic acid probe, test kit and diagnostic and purification methods
    2.
    发明授权
    Nucleic acid probe, test kit and diagnostic and purification methods 失效
    核酸探针，测试试剂盒和诊断和纯化方法

    公开(公告)号：US5328825A

    公开(公告)日：1994-07-12

    申请号：US847447

    申请日：1992-03-06

    申请人： Harold C. Warren, III ,  John B. Findlay ,  Marlene M. King

    发明人： Harold C. Warren, III ,  John B. Findlay ,  Marlene M. King

    IPC分类号： C12N15/09 ,  C12Q1/68 ,  C12Q1/70 ,  G01N33/543 ,  C07H21/04

    CPC分类号： G01N33/54313 ,  C12Q1/6834 ,  C12Q1/703

    摘要： An oligonucleotide is linked to a particle through a protein or carbohydrate to form a water-insoluble nucleic acid probe. The protein or carbohydrate has a pI of about 6 or less, and has been chemically modified with an acylating, alkylating or sulfonylating agent. The particle surface is substantially free of other proteins or carbohydrates. The probe is useful in various diagnostic and purification methods wherein hybridization of the oligonucleotide with a target nucleic acid is possible. In one instance, the probe can be used to capture a DNA strand which has been amplified using polymerase chain reaction techniques.

    摘要翻译： 寡核苷酸通过蛋白质或碳水化合物与颗粒连接以形成水不溶性核酸探针。 蛋白质或碳水化合物具有约6或更小的pI，并已用酰化，烷基化或磺酰化剂进行化学修饰。 颗粒表面基本上不含其他蛋白质或碳水化合物。 探针可用于各种诊断和纯化方法，其中寡核苷酸与靶核酸的杂交是可能的。 在一个实例中，探针可用于捕获已经使用聚合酶链反应技术扩增的DNA链。

    Avidin-and biotin immobilized reagents and methods of use
    3.
    发明授权
    Avidin-and biotin immobilized reagents and methods of use 失效
    抗生物素蛋白和生物素固定化试剂及使用方法

    公开(公告)号：US5374516A

    公开(公告)日：1994-12-20

    申请号：US315086

    申请日：1989-02-24

    申请人： Richard C. Sutton ,  Brent A. Burdick ,  Susan J. Danielson ,  Harold C. Warren, III ,  Brian A. Snyder ,  Gregory J. McClune ,  Annie L. Wu

    发明人： Richard C. Sutton ,  Brent A. Burdick ,  Susan J. Danielson ,  Harold C. Warren, III ,  Brian A. Snyder ,  Gregory J. McClune ,  Annie L. Wu

    IPC分类号： G01N33/543 ,  G01N33/545 ,  G01N33/546

    CPC分类号： G01N33/54306 ,  G01N33/54313 ,  G01N33/545 ,  Y10T428/2991 ,  Y10T428/2998

    摘要： Reagents have been prepared from water-insoluble polymeric particles to which are covalently attached avidin, biotin or an avidin or biotin derivative. The polymeric particles comprise a polymer on at least the outer surface which is derived from at least one ethylenically unsaturated monomer having a reactive activated 2-substituted ethylsulfonyl, vinylsulfonyl or active halogen atom. Covalent attachment of avidin, biotin or an avidin or biotin derivative is effected either directly or indirectly through these reactive groups. The resulting reagent is useful in analytical elements and various analytical methods including agglutination and sandwich assays. The immobilized avidin, biotin or derivative can be used to complex with the corresponding biotin or avidin molecule which may be conjugated to a compound of biological interest.

    摘要翻译： 试剂由水不溶性聚合物颗粒制备，共价连接的抗生物素蛋白，生物素或抗生物素蛋白或生物素衍生物。 聚合物颗粒在至少外表面上包含衍生自至少一种具有反应性活化的2-取代的乙基磺酰基，乙烯基磺酰基或活性卤素原子的烯属不饱和单体的聚合物。 亲和素，生物素或抗生物素蛋白或生物素衍生物的共价连接通过这些反应性基团直接或间接地实现。 所得试剂可用于分析元件和各种分析方法，包括凝集和夹心测定。 固定的抗生物素蛋白，生物素或衍生物可用于与可能与生物学兴趣的化合物缀合的相应生物素或抗生物素蛋白分子复合。

    Membrane structure coated with low pI protein or carbohydrate and methods of making and use
    9.
    发明授权
    Membrane structure coated with low pI protein or carbohydrate and methods of making and use 失效
    涂有低pI蛋白或碳水化合物的膜结构及制造和使用方法

    公开(公告)号：US4828980A

    公开(公告)日：1989-05-09

    申请号：US98433

    申请日：1987-09-18

    申请人： Brian A. Snyder ,  Harold C. Warren, III ,  Roger W. Nelson

    发明人： Brian A. Snyder ,  Harold C. Warren, III ,  Roger W. Nelson

    IPC分类号： B01D67/00 ,  G01N33/543 ,  G01N33/544 ,  G01N33/548

    CPC分类号： B01D67/0088 ,  G01N33/54393 ,  G01N33/544 ,  G01N33/548 ,  Y10S436/807 ,  Y10S436/824 ,  Y10T428/249978 ,  Y10T428/249979 ,  Y10T428/24998

    摘要： A membrane structure useful in filtration and diagnostic tests includes a microporous membrane formed from a biologically inert material, such as a polyamide, and has a coating comprising one or more water-soluble proteins or carbohydrates. None of the proteins and carbohydrates in the coating has a pI greater than about 5. The membrane structure is prepared by contacting the microporous membrane with the appropriate protein or carbohydrate in an amount sufficient to provide a coating over the entire membrane surface without substantially diminishing the porosity of the membrane. The membrane structure is useful in various diagnostic test procedures, such as agglutination assays.

    摘要翻译： 用于过滤和诊断测试的膜结构包括由生物惰性材料（例如聚酰胺）形成的微孔膜，并且具有包含一种或多种水溶性蛋白质或碳水化合物的涂层。 涂层中的蛋白质和碳水化合物中没有一个pI大于约5.膜结构通过使微孔膜与适当的蛋白质或碳水化合物接触而制备，其量足以在整个膜表面上提供涂层，而基本上不会减少 膜的孔隙率。 膜结构可用于各种诊断测试程序，例如凝集测定。

    Analytical method and element for protein assay
    10.
    发明授权
    Analytical method and element for protein assay 失效
    蛋白质分析方法和元素

    公开(公告)号：US4786605A

    公开(公告)日：1988-11-22

    申请号：US64640

    申请日：1987-06-22

    申请人： John C. Mauck ,  Harold C. Warren, III

    发明人： John C. Mauck ,  Harold C. Warren, III

    IPC分类号： G01N33/52 ,  G01N33/68 ,  G01N33/00 ,  G01N21/00

    CPC分类号： G01N33/6833 ,  G01N33/526

    摘要： There is disclosed a method for quantitatively determining protein, comprising the steps of:(a) providing a sample of the protein in an aqueous medium;(b) providing an aqueous medium having a pH in excess of 12 and comprising(i) a cupric salt and a pyridyl-azo dye; or(ii) a preformed cupric-pyridylazo dye complex;(c) combining the aqueous mediums of a) and b) thereby providing a color having an intensity which is inversely proportional to the amount of unreacted dye present in the combined mediums; and(d) determining the quantity of protein present in the sample colorimetrically.The method can be used with a multilayer dry analytical element.