利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

3 条记录 (耗时 0.041 秒)

    Modified photosynthetic microorganisms for producing lipids
    1.
    发明授权
    Modified photosynthetic microorganisms for producing lipids 有权
    用于生产脂质的改良光合微生物

    公开(公告)号：US09523096B2

    公开(公告)日：2016-12-20

    申请号：US13995925

    申请日：2011-12-19

    申请人： James Roberts ,  Fred Cross ,  Margaret Mary McCormick ,  Ernesto Javier Munoz ,  Brett K. Kaiser ,  Michael Carleton

    发明人： James Roberts ,  Fred Cross ,  Margaret Mary McCormick ,  Ernesto Javier Munoz ,  Brett K. Kaiser ,  Michael Carleton

    IPC分类号： C12N1/00 ,  C12N1/21 ,  C12N15/82 ,  C07K14/195 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16 ,  C12N9/20 ,  C12N9/90 ,  C12N9/00 ,  C12P7/64

    CPC分类号： C12N15/82 ,  C07K14/195 ,  C12N9/1029 ,  C12N9/12 ,  C12N9/16 ,  C12N9/20 ,  C12N9/90 ,  C12N9/93 ,  C12P7/64 ,  Y02P20/52

    摘要： This disclosure describes genetically modified photosynthetic microorganisms, e.g., Cyanobacteria, that overexpress an acyl carrier protein (ACP), an acyl-ACP synthase (Aas), or both, optionally in combination with one or more overexpressed or exogenous lipid biosynthesis proteins, and/or one or more overexpressed or exogenous glycogen breakdown proteins. Exemplary biosynthesis proteins include diacyglycerol acyltransferases, thioesterases, phosphatidate phosphatases, phospholipases, triacylglycerol (TAG) hydrolases, fatty acyl-CoA synthetases, and/or acetyl-CoA carboxylases, including combinations thereof. Also included are photosynthetic microorganisms comprising mutations or deletions in a glycogen biosynthesis or storage pathway, which accumulate a reduced amount of glycogen under reduced nitrogen conditions as compared to a wild type photosynthetic microorganism. The modified photosynthetic microorganisms provided herein are capable of producing increased amounts of lipids such as fatty acids and/or synthesizing triglycerides.

    摘要翻译： 本公开描述了任选与一种或多种过表达或外源脂质生物合成蛋白组合的转基因修饰的光合微生物，例如过度表达酰基载体蛋白（ACP），酰基-ACP合酶（Aas）或两者的蓝细菌，和/ 或一种或多种过表达或外源糖原分解蛋白。 示例性生物合成蛋白包括二酰甘油酰基转移酶，硫酯酶，磷脂酸磷酸酶，磷脂酶，三酰基甘油（TAG）水解酶，脂肪酰辅酶A合成酶和/或乙酰辅酶A羧化酶，包括其组合。 还包括光合微生物，其包含糖原生物合成或储存途径中的突变或缺失，与野生型光合微生物相比，其在降低的氮条件下积累减少量的糖原。 本文提供的修饰的光合微生物能够产生增加量的脂质，例如脂肪酸和/或合成甘油三酯。

    MODIFIED PHOTOSYNTHETIC MICROORGANISMS FOR PRODUCING LIPIDS
    2.
    发明申请
    MODIFIED PHOTOSYNTHETIC MICROORGANISMS FOR PRODUCING LIPIDS 有权
    用于生产脂肪的改性光合微生物

    公开(公告)号：US20130344549A1

    公开(公告)日：2013-12-26

    申请号：US13995925

    申请日：2011-12-19

    申请人： James Roberts ,  Fred Cross ,  Margaret Mary McCormick ,  Ernesto Javier Munoz ,  Brett K. Kaiser ,  Michael Carleton

    发明人： James Roberts ,  Fred Cross ,  Margaret Mary McCormick ,  Ernesto Javier Munoz ,  Brett K. Kaiser ,  Michael Carleton

    IPC分类号： C12P7/64 ,  C12N15/82

    CPC分类号： C12N15/82 ,  C07K14/195 ,  C12N9/1029 ,  C12N9/12 ,  C12N9/16 ,  C12N9/20 ,  C12N9/90 ,  C12N9/93 ,  C12P7/64 ,  Y02P20/52

    摘要： This disclosure describes genetically modified photosynthetic microorganisms, e.g., Cyanobacteria, that overexpress an acyl carrier protein (ACP), an acyl-ACP synthase (Aas), or both, optionally in combination with one or more overexpressed or exogenous lipid biosynthesis proteins, and/or one or more overexpressed or exogenous glycogen breakdown proteins. Exemplary biosynthesis proteins include diacyglycerol acyltransferases, thioesterases, phosphatidate phosphatases, phospholipases, triacylglycerol (TAG) hydrolases, fatty acyl-CoA synthetases, and/or acetyl-CoA carboxylases, including combinations thereof. Also included are photosynthetic microorganisms comprising mutations or deletions in a glycogen biosynthesis or storage pathway, which accumulate a reduced amount of glycogen under reduced nitrogen conditions as compared to a wild type photosynthetic microorganism. The modified photosynthetic microorganisms provided herein are capable of producing increased amounts of lipids such as fatty acids and/or synthesizing triglycerides.

    摘要翻译： 本公开描述了任选与一种或多种过表达或外源脂质生物合成蛋白组合的转基因修饰的光合微生物，例如过度表达酰基载体蛋白（ACP），酰基-ACP合酶（Aas）或两者的蓝细菌，和/ 或一种或多种过表达或外源糖原分解蛋白。 示例性生物合成蛋白包括二酰甘油酰基转移酶，硫酯酶，磷脂酸磷酸酶，磷脂酶，三酰基甘油（TAG）水解酶，脂肪酰辅酶A合成酶和/或乙酰辅酶A羧化酶，包括其组合。 还包括光合微生物，其包含糖原生物合成或储存途径中的突变或缺失，与野生型光合微生物相比，其在降低的氮条件下积累减少量的糖原。 本文提供的修饰的光合微生物能够产生增加量的脂质，例如脂肪酸和/或合成甘油三酯。