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公开(公告)号:US20060189795A1
公开(公告)日:2006-08-24
申请号:US10547568
申请日:2004-03-18
CPC分类号: B01D15/388 , B01D15/166 , B01D15/20 , B01D15/327 , B01J20/285
摘要: The present invention relates to a method of isolating target compounds from a liquid, which comprises at a first pH, contacting the liquid with a separation medium that exhibits surface-localised pH-responsive polymers in to adsorb the target compound via hydrophobic interactions; and adding an eluent, which is of a second pH and provides a conformational change of said pH-responsive polymers to release said compounds. The elution is advantageously performed by a pH gradient and/or by a salt gradient.
摘要翻译: 本发明涉及一种从液体中分离目标化合物的方法,该方法包括在第一pH下,使液体与分离介质接触,所述分离介质显示表面定位的pH响应性聚合物以通过疏水相互作用吸附目标化合物; 并加入洗脱液,其为第二pH,并提供所述pH响应聚合物的构象变化以释放所述化合物。 洗脱有利地通过pH梯度和/或盐梯度进行。
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公开(公告)号:US20100059440A1
公开(公告)日:2010-03-11
申请号:US12523323
申请日:2008-02-07
申请人: Asa Rudstedt , Andreas Axen , Nils Norrman , Anders Larsson
发明人: Asa Rudstedt , Andreas Axen , Nils Norrman , Anders Larsson
CPC分类号: B01D71/10 , B01D15/36 , B01D15/3804 , B01D67/0093 , B01D71/16 , B01D2323/30 , B01D2325/14 , B01D2325/16 , B01J20/262 , B01J20/265 , B01J20/267 , B01J20/28033 , B01J20/28085 , B01J20/285 , B01J20/286 , B01J20/3242 , B01J20/3244
摘要: The invention relates to a process for making porous cross-linked charged cellulosic polymeric membranes capable of binding to a target molecule. The invention provides methods for separating target molecules from other components in a solution comprising use of membranes obtainable by the process of the invention. The method has particular utility in separating proteins and nucleic acids from cell lysates and cultures.
摘要翻译: 本发明涉及一种制备能够结合靶分子的多孔交联带电纤维素聚合物膜的方法。 本发明提供了在包含使用可通过本发明的方法获得的膜的溶液中从目标分子与其它组分分离的方法。 该方法在从细胞裂解物和培养物中分离蛋白质和核酸方面具有特殊的用途。
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公开(公告)号:US09522365B2
公开(公告)日:2016-12-20
申请号:US12523323
申请日:2008-02-07
申请人: Asa Rudstedt , Andreas Axen , Nils Norrman , Anders Larsson
发明人: Asa Rudstedt , Andreas Axen , Nils Norrman , Anders Larsson
IPC分类号: B01D71/16 , B01J20/26 , B01J20/28 , B01J20/285 , B01J20/286 , B01J20/32 , B01D71/10 , B01D67/00 , B01D61/14 , B01D15/36 , B01D15/38
CPC分类号: B01D71/10 , B01D15/36 , B01D15/3804 , B01D67/0093 , B01D71/16 , B01D2323/30 , B01D2325/14 , B01D2325/16 , B01J20/262 , B01J20/265 , B01J20/267 , B01J20/28033 , B01J20/28085 , B01J20/285 , B01J20/286 , B01J20/3242 , B01J20/3244
摘要: The invention relates to a process for making porous cross-linked charged cellulosic polymeric membranes capable of binding to a target molecule. The invention provides methods for separating target molecules from other components in a solution comprising use of membranes obtainable by the process of the invention. The method has particular utility in separating proteins and nucleic acids from cell lysates and cultures.
摘要翻译: 本发明涉及一种制备能够结合靶分子的多孔交联带电纤维素聚合物膜的方法。 本发明提供了在包含使用可通过本发明的方法获得的膜的溶液中从目标分子与其它组分分离的方法。 该方法在从细胞裂解物和培养物中分离蛋白质和核酸方面具有特殊的用途。
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