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公开(公告)号：US09399658B2
公开(公告)日：2016-07-26
申请号：US14007752
申请日：2012-03-28
申请人： Janos Ludwig , Marion Goldeck , Brian Sproat
发明人： Janos Ludwig , Marion Goldeck , Brian Sproat
CPC分类号： C12N15/113 , C07H1/02 , C07H1/04 , C07H21/00 , C07H21/02 , C12N2330/30
摘要： The present invention relates to a method of preparing triphosphate-modified oligonucleotides using a capture tag. The method allows the synthesis and purification of triphosphate-modified oligonucleotides in high yield and purity suitable for pharmaceutical applications.
摘要翻译： 本发明涉及使用捕获标签制备三磷酸修饰的寡核苷酸的方法。 该方法允许以高产率和纯度合成和纯化三磷酸修饰的寡核苷酸，适用于药物应用。
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公开(公告)号：US20140024819A1
公开(公告)日：2014-01-23
申请号：US14007752
申请日：2012-03-28
申请人： Janos Ludwig , Marion Goldeck , Brian Sproat
发明人： Janos Ludwig , Marion Goldeck , Brian Sproat
CPC分类号： C12N15/113 , C07H1/02 , C07H1/04 , C07H21/00 , C07H21/02 , C12N2330/30
摘要： The present invention relates to a method of preparing triphosphate-modified oligonucleotides using a capture tag. The method allows the synthesis and purification of triphosphate-modified oligonucleotides in high yield and purity suitable for pharmaceutical applications.
摘要翻译： 本发明涉及使用捕获标签制备三磷酸修饰的寡核苷酸的方法。 该方法允许以高产率和纯度合成和纯化三磷酸修饰的寡核苷酸，适用于药物应用。
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公开(公告)号：US08383370B2
公开(公告)日：2013-02-26
申请号：US12525176
申请日：2008-01-30
申请人： Thomas Tuschl , Janos Ludwig , Yi Pei , Carolina Lin
发明人： Thomas Tuschl , Janos Ludwig , Yi Pei , Carolina Lin
摘要： The invention provides a novel truncated mutated T4 RNA ligase 2. In addition, methods are provided for ligating pre-adenlylated donor molecules to the 3′ hydroxyl group of RNA in the absence of ATP using the ligase.
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公开(公告)号：US20110244523A1
公开(公告)日：2011-10-06
申请号：US12525176
申请日：2008-01-30
申请人： Thomas Tuschl , Janos Ludwig , Yi Pei , Carolina P. Lin
发明人： Thomas Tuschl , Janos Ludwig , Yi Pei , Carolina P. Lin
摘要： The invention provides a novel truncated mutated T4 RNA ligase 2. In addition, methods are provided for ligating pre-adenlylated donor molecules to the 3′ hydroxyl group of RNA in the absence of ATP using the ligase.
摘要翻译： 本发明提供了一种新的截短突变T4 RNA连接酶2.此外，提供了使用连接酶在不存在ATP的情况下将前腺苷酰供体分子连接到RNA的3'羟基的方法。
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公开(公告)号：US08394588B2
公开(公告)日：2013-03-12
申请号：US12721550
申请日：2010-03-10
申请人： Thomas Tuschl , John Pena , Cherin Sohn , Sara Hakim , Janos Ludwig , Pavol Cekan
发明人： Thomas Tuschl , John Pena , Cherin Sohn , Sara Hakim , Janos Ludwig , Pavol Cekan
CPC分类号： G01N1/30 , C12Q1/6806 , C12Q1/6841 , C12Q2525/207 , C12Q2523/101
摘要： In one aspect, the invention relates to a method for fixing a short nucleic acid in a biological sample. In another aspect, the invention relates to a method for detecting a target short nucleic acid in a biological sample. The method includes contacting the biological sample with an aldehyde-containing fixative, and subsequently contacting the sample with a water-soluble carbodiimide. In a further aspect, the invention relates to a kit for fixing a short nucleic acid in a biological sample. The kit includes a support substrate for holding the sample; an aldehyde-containing fixative; and a water-soluble carbodiimide.
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公开(公告)号：US06656731B1
公开(公告)日：2003-12-02
申请号：US09479005
申请日：2000-01-07
申请人： Fritz Eckstein , Janos Ludwig , Leonid Beigelman
发明人： Fritz Eckstein , Janos Ludwig , Leonid Beigelman
IPC分类号： C12N500
CPC分类号： C12N15/113 , A61K38/00 , C12N2310/111 , C12N2310/121 , C12N2310/18
摘要： The present invention relates to nucleic acid molecules with new motifs having catalytic activity, methods of syntheses and uses thereof.
摘要翻译： 本发明涉及具有催化活性的新基序的核酸分子，其合成方法及其应用。
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