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    METHODS FOR THE IDENTIFICATION OF ZAP-70 INTERACTING MOLECULES AND FOR THE PURIFICATION OF ZAP-70
    3.
    发明申请
    METHODS FOR THE IDENTIFICATION OF ZAP-70 INTERACTING MOLECULES AND FOR THE PURIFICATION OF ZAP-70 审中-公开

    公开(公告)号：US20120178180A1

    公开(公告)日：2012-07-12

    申请号：US12302711

    申请日：2007-06-01

    申请人： Ulrich Kruse ,  Nigel Ramsden ,  Gerard Drewes ,  Dirk Eberhard

    发明人： Ulrich Kruse ,  Nigel Ramsden ,  Gerard Drewes ,  Dirk Eberhard

    IPC分类号： G01N33/53 ,  G01N30/02

    CPC分类号： C12Q1/485 ,  C12N9/1205 ,  G01N33/57426 ,  G01N2333/91215 ,  G01N2500/02 ,  G01N2500/10

    摘要： The invention provides in a first aspect a method for the identification of an ZAP-70 interacting compound, comprising the steps of a) providing a protein preparation containing phosphorylated ZAP-70, b) contacting the protein preparation with aminopyrido-pyrimidine ligand 24 immobilized on a solid support under conditions allowing the formation of an aminopyrido-pyrimidine ligand 24—phosphorylated ZAP-70 complex, c) incubating the aminopyrido-pyrimidine ligand 24—phosphorylated ZAP-70 complex with a given compound, and d) determining whether the compound is able to separate phosphorylated ZAP-70 from the immobilized aminopyrido-pyrimidine ligand 24. In a second aspect, the present invention relates to A method for the identification of an ZAP-70 interacting compound, comprising the steps of a) providing a protein preparation containing phosphorylated ZAP-70, b) contacting the protein preparation with ligand aminopyrido-pyrimidine ligand 24 immobilized on a solid support and with a given compound under conditions allowing the formation of an aminopyrido-pyrimidine ligand 24—phosphorylated ZAP-70 complex, and c) detecting the aminopyrido-pyrimidine ligand 24—phosphorylated ZAP-70 complex formed in step b). In a third aspect, the invention provides a method for the identification of an ZAP-70 interacting compound, comprising the steps of: a) providing two aliquots of a protein preparation containing phosphorylated ZAP-70, b) contacting one aliquot with aminopyrido-pyrimidine ligand 24 immobilized on a solid support under conditions allowing the formation of an aminopyrido-pyrimidine ligand 24—phosphorylated ZAP-70 complex, c) contacting the other aliquot with aminopyrido-pyrimidine ligand 24 immobilized on a solid support and with a given compound under conditions allowing the formation of an aminopyrido-pyrimidine ligand 24—phosphorylated ZAP-70 complex, and d) determining the amount of aminopyrido-pyrimidine ligand 24—phosphorylated ZAP-70 complex formed in steps b) and c). In a forth aspect, the invention relates to a method for the identification of an ZAP-70 interacting compound, comprising the steps of: a) providing two aliquots comprising each at least one cell containing phosphorylated ZAP-70, b) incubating one aliquot with a given compound, c) harvesting the cells of each aliquot, d) lysing the cells in order to obtain protein preparations, e) contacting the protein preparations with aminopyrido-pyrimidine ligand 24 immobilized on a solid support under conditions allowing the formation of an aminopyrido-pyrimidine ligand 24—phosphorylated ZAP-70 complex, and f) determining the amount of aminopyrido-pyrimidine ligand 24—phosphorylated ZAP-70 complex formed in each aliquot in step e).

    IN VIVO METHOD FOR THE EVALUATION OF A COMPOUND-TARGET INTERACTION
    5.
    发明申请
    IN VIVO METHOD FOR THE EVALUATION OF A COMPOUND-TARGET INTERACTION 审中-公开
    用于评估化合物 - 目标相互作用的体内方法

    公开(公告)号：US20130210030A1

    公开(公告)日：2013-08-15

    申请号：US13809811

    申请日：2011-06-20

    申请人： Gerard Drewes ,  Carsten Hopf ,  Gitte Neubauer ,  Ulrich Kruse

    发明人： Gerard Drewes ,  Carsten Hopf ,  Gitte Neubauer ,  Ulrich Kruse

    IPC分类号： G01N33/573

    CPC分类号： G01N33/573 ,  G01N33/5088 ,  G01N2333/91215 ,  G01N2500/00

    摘要： The invention relates to a method for evaluation of a compound-target interaction in vivo, comprising the steps of administering the compound to an animal, taking a body fluid sample of the animal, determining the concentration of the compound in the body fluid sample, taking a cellular sample of the animal containing the target, preparing a protein preparation of said cellular sample, providing an immobilised ligand capable of binding to the target, contacting the protein preparation with the immobilised ligand under conditions allowing the formation of a complex between the immobilised ligand and the target, determining the amount of complexes formed in step, and correlating the amount of complexes with the concentration of the compound in the body fluid sample.

    摘要翻译： 本发明涉及一种用于评价体内化合物 - 靶相互作用的方法，包括以下步骤：向动物施用化合物，服用动物的体液样品，测定体液样品中化合物的浓度，取 制备含有靶标的动物的细胞样品，制备所述细胞样品的蛋白质制剂，提供能够结合靶标的固定配体，使蛋白质制剂与固定的配体在允许形成固定化配体之间的复合物的条件下接触 和靶标，测定步骤中形成的复合物的量，并将复合物的量与体液样品中化合物的浓度相关联。

    Process for the Identification of New Medical Targets
    7.
    发明申请
    Process for the Identification of New Medical Targets 审中-公开
    确定新医疗目标的过程

    公开(公告)号：US20070269830A1

    公开(公告)日：2007-11-22

    申请号：US10555896

    申请日：2004-05-07

    申请人： Giulio Superti-Fuga ,  Ulrich Kruse ,  Vladimir Rybin ,  Gitte Neubauer

    发明人： Giulio Superti-Fuga ,  Ulrich Kruse ,  Vladimir Rybin ,  Gitte Neubauer

    IPC分类号： C12Q1/68 ,  G01N33/53

    CPC分类号： G01N33/543 ,  G01N33/6803 ,  G01N33/94 ,  G01N2333/525 ,  G01N2500/04

    摘要： The present invention relates to a process for the isolation and identification of pharmaceutically relevant target compounds (TC) from a sample, said sample being derived from a living organism, wherein said target compound(s) bind(s) to a compound of interest (COI) under physiological conditions, said compound of interest (COI) being associated with a given impaired condition or disease. Furthermore, the present invention relates to a process for the identification of a pharmaceutically effective compound useful for preventing and/or treating a given impaired condition or disease, wherein said compound is identified by its binding to a relevant target compound (TC) that has been identified and isolated according to the above mentioned process.

    摘要翻译： 本发明涉及从样品中分离和鉴定药学上相关的目标化合物（TC）的方法，所述样品衍生自活生物体，其中所述目标化合物与感兴趣的化合物结合（ COI），所述感兴趣的化合物（COI）与给定的受损条件或疾病相关。 此外，本发明涉及用于鉴定可用于预防和/或治疗给定的受损病症或疾病的药学上有效的化合物的方法，其中所述化合物通过与已经经过的相关目标化合物（TC）的结合来鉴定 根据上述过程鉴定和分离。